Domain-interface dynamics of CFTR revealed by stabilizing nanobodies

The leading cause of cystic fibrosis is the deletion of phenylalanine 508 (F508del) in the first nucleotide-binding domain (NBD1) of the cystic fibrosis transmembrane conductance regulator (CFTR). Here authors we develop nanobodies targeting NBD1 of human CFTR and demonstrate their ability to stabil...

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Autores principales:	Maud Sigoillot, Marie Overtus, Magdalena Grodecka, Daniel Scholl, Abel Garcia-Pino, Toon Laeremans, Lihua He, Els Pardon, Ellen Hildebrandt, Ina Urbatsch, Jan Steyaert, John R. Riordan, Cedric Govaerts
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/2a36a5cc137946bdbc014d5d58e302cb
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spelling	oai:doaj.org-article:2a36a5cc137946bdbc014d5d58e302cb2021-12-02T16:57:30ZDomain-interface dynamics of CFTR revealed by stabilizing nanobodies10.1038/s41467-019-10714-y2041-1723https://doaj.org/article/2a36a5cc137946bdbc014d5d58e302cb2019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10714-yhttps://doaj.org/toc/2041-1723The leading cause of cystic fibrosis is the deletion of phenylalanine 508 (F508del) in the first nucleotide-binding domain (NBD1) of the cystic fibrosis transmembrane conductance regulator (CFTR). Here authors we develop nanobodies targeting NBD1 of human CFTR and demonstrate their ability to stabilize both isolated NBD1 and full-length protein.Maud SigoillotMarie OvertusMagdalena GrodeckaDaniel SchollAbel Garcia-PinoToon LaeremansLihua HeEls PardonEllen HildebrandtIna UrbatschJan SteyaertJohn R. RiordanCedric GovaertsNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Maud Sigoillot Marie Overtus Magdalena Grodecka Daniel Scholl Abel Garcia-Pino Toon Laeremans Lihua He Els Pardon Ellen Hildebrandt Ina Urbatsch Jan Steyaert John R. Riordan Cedric Govaerts Domain-interface dynamics of CFTR revealed by stabilizing nanobodies
description	The leading cause of cystic fibrosis is the deletion of phenylalanine 508 (F508del) in the first nucleotide-binding domain (NBD1) of the cystic fibrosis transmembrane conductance regulator (CFTR). Here authors we develop nanobodies targeting NBD1 of human CFTR and demonstrate their ability to stabilize both isolated NBD1 and full-length protein.
format	article
author	Maud Sigoillot Marie Overtus Magdalena Grodecka Daniel Scholl Abel Garcia-Pino Toon Laeremans Lihua He Els Pardon Ellen Hildebrandt Ina Urbatsch Jan Steyaert John R. Riordan Cedric Govaerts
author_facet	Maud Sigoillot Marie Overtus Magdalena Grodecka Daniel Scholl Abel Garcia-Pino Toon Laeremans Lihua He Els Pardon Ellen Hildebrandt Ina Urbatsch Jan Steyaert John R. Riordan Cedric Govaerts
author_sort	Maud Sigoillot
title	Domain-interface dynamics of CFTR revealed by stabilizing nanobodies
title_short	Domain-interface dynamics of CFTR revealed by stabilizing nanobodies
title_full	Domain-interface dynamics of CFTR revealed by stabilizing nanobodies
title_fullStr	Domain-interface dynamics of CFTR revealed by stabilizing nanobodies
title_full_unstemmed	Domain-interface dynamics of CFTR revealed by stabilizing nanobodies
title_sort	domain-interface dynamics of cftr revealed by stabilizing nanobodies
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/2a36a5cc137946bdbc014d5d58e302cb
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Domain-interface dynamics of CFTR revealed by stabilizing nanobodies

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