HSP90 and HSP70 Families in Lateolabrax maculatus: Genome-Wide Identification, Molecular Characterization, and Expression Profiles in Response to Various Environmental Stressors

Heat shock proteins (HSPs) are a large class of highly conserved chaperons, which play important roles in response to elevated temperature and other environmental stressors. In the present study, 5 HSP90 genes and 17 HSP70 genes were systematically characterized in spotted seabass (Lateolabrax macul...

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Autores principales:	Yalong Sun, Haishen Wen, Yuan Tian, Xuebin Mao, Xiurong Li, Junjie Li, Yanbo Hu, Yang Liu, Jifang Li, Yun Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	HSP90 HSP70 spotted seabass environmental stresses gene expression transcriptome Physiology QP1-981
Acceso en línea:	https://doaj.org/article/2a4035f1fb5f4c7a92ea0ced4c2cd259
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spelling	oai:doaj.org-article:2a4035f1fb5f4c7a92ea0ced4c2cd2592021-11-22T07:27:46ZHSP90 and HSP70 Families in Lateolabrax maculatus: Genome-Wide Identification, Molecular Characterization, and Expression Profiles in Response to Various Environmental Stressors1664-042X10.3389/fphys.2021.784803https://doaj.org/article/2a4035f1fb5f4c7a92ea0ced4c2cd2592021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fphys.2021.784803/fullhttps://doaj.org/toc/1664-042XHeat shock proteins (HSPs) are a large class of highly conserved chaperons, which play important roles in response to elevated temperature and other environmental stressors. In the present study, 5 HSP90 genes and 17 HSP70 genes were systematically characterized in spotted seabass (Lateolabrax maculatus). The evolutionary footprint of HSP genes was revealed via the analysis of phylogeny, chromosome location, and gene copy numbers. In addition, the gene structure features and the putative distribution of heat shock elements (HSEs) and hypoxia response elements (HREs) in the promoter regions were analyzed. The protein-protein interaction (PPI) network analyses results indicated the potential transcriptional regulation between the heat shock factor 1 (HSF1) and HSPs and a wide range of interactions among HSPs. Furthermore, quantitative (q)PCR was performed to detect the expression profiles of HSP90 and HSP70 genes in gill, liver, and muscle tissues after heat stress, meanwhile, the expression patterns in gills under alkalinity and hypoxia stresses were determined by analyzing RNA-Seq datasets. Results showed that after heat stress, most of the examined HSP genes were significantly upregulated in a tissue-specific and time-dependent manners, and hsp90aa1.1, hsp90aa1.2, hsp70.1, and hsp70.2 were the most intense responsive genes in all three tissues. In response to alkalinity stress, 11 out of 13 significantly regulated HSP genes exhibited suppressed expression patterns. Alternatively, among the 12 hypoxia-responsive-expressed HSP genes, 7 genes showed induced expressions, while hsp90aa1.2, hsp70.1, and hsp70.2 had more significant upregulated changes after hypoxic challenge. Our findings provide the essential basis for further functional studies of HSP genes in response to abiotic stresses in spotted seabass.Yalong SunHaishen WenYuan TianXuebin MaoXiurong LiJunjie LiYanbo HuYang LiuJifang LiYun LiFrontiers Media S.A.articleHSP90HSP70spotted seabassenvironmental stressesgene expressiontranscriptomePhysiologyQP1-981ENFrontiers in Physiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	HSP90 HSP70 spotted seabass environmental stresses gene expression transcriptome Physiology QP1-981
spellingShingle	HSP90 HSP70 spotted seabass environmental stresses gene expression transcriptome Physiology QP1-981 Yalong Sun Haishen Wen Yuan Tian Xuebin Mao Xiurong Li Junjie Li Yanbo Hu Yang Liu Jifang Li Yun Li HSP90 and HSP70 Families in Lateolabrax maculatus: Genome-Wide Identification, Molecular Characterization, and Expression Profiles in Response to Various Environmental Stressors
description	Heat shock proteins (HSPs) are a large class of highly conserved chaperons, which play important roles in response to elevated temperature and other environmental stressors. In the present study, 5 HSP90 genes and 17 HSP70 genes were systematically characterized in spotted seabass (Lateolabrax maculatus). The evolutionary footprint of HSP genes was revealed via the analysis of phylogeny, chromosome location, and gene copy numbers. In addition, the gene structure features and the putative distribution of heat shock elements (HSEs) and hypoxia response elements (HREs) in the promoter regions were analyzed. The protein-protein interaction (PPI) network analyses results indicated the potential transcriptional regulation between the heat shock factor 1 (HSF1) and HSPs and a wide range of interactions among HSPs. Furthermore, quantitative (q)PCR was performed to detect the expression profiles of HSP90 and HSP70 genes in gill, liver, and muscle tissues after heat stress, meanwhile, the expression patterns in gills under alkalinity and hypoxia stresses were determined by analyzing RNA-Seq datasets. Results showed that after heat stress, most of the examined HSP genes were significantly upregulated in a tissue-specific and time-dependent manners, and hsp90aa1.1, hsp90aa1.2, hsp70.1, and hsp70.2 were the most intense responsive genes in all three tissues. In response to alkalinity stress, 11 out of 13 significantly regulated HSP genes exhibited suppressed expression patterns. Alternatively, among the 12 hypoxia-responsive-expressed HSP genes, 7 genes showed induced expressions, while hsp90aa1.2, hsp70.1, and hsp70.2 had more significant upregulated changes after hypoxic challenge. Our findings provide the essential basis for further functional studies of HSP genes in response to abiotic stresses in spotted seabass.
format	article
author	Yalong Sun Haishen Wen Yuan Tian Xuebin Mao Xiurong Li Junjie Li Yanbo Hu Yang Liu Jifang Li Yun Li
author_facet	Yalong Sun Haishen Wen Yuan Tian Xuebin Mao Xiurong Li Junjie Li Yanbo Hu Yang Liu Jifang Li Yun Li
author_sort	Yalong Sun
title	HSP90 and HSP70 Families in Lateolabrax maculatus: Genome-Wide Identification, Molecular Characterization, and Expression Profiles in Response to Various Environmental Stressors
title_short	HSP90 and HSP70 Families in Lateolabrax maculatus: Genome-Wide Identification, Molecular Characterization, and Expression Profiles in Response to Various Environmental Stressors
title_full	HSP90 and HSP70 Families in Lateolabrax maculatus: Genome-Wide Identification, Molecular Characterization, and Expression Profiles in Response to Various Environmental Stressors
title_fullStr	HSP90 and HSP70 Families in Lateolabrax maculatus: Genome-Wide Identification, Molecular Characterization, and Expression Profiles in Response to Various Environmental Stressors
title_full_unstemmed	HSP90 and HSP70 Families in Lateolabrax maculatus: Genome-Wide Identification, Molecular Characterization, and Expression Profiles in Response to Various Environmental Stressors
title_sort	hsp90 and hsp70 families in lateolabrax maculatus: genome-wide identification, molecular characterization, and expression profiles in response to various environmental stressors
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2a4035f1fb5f4c7a92ea0ced4c2cd259
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HSP90 and HSP70 Families in Lateolabrax maculatus: Genome-Wide Identification, Molecular Characterization, and Expression Profiles in Response to Various Environmental Stressors

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