Metabolomics for early detection of stress in freshwater alga Poterioochromonas malhamensis exposed to silver nanoparticles

Abstract Silver nanoparticles (AgNPs) are one of the most used engineered nanomaterials. Despite progress in assessing their environmental implications, knowledge gaps exist concerning the metabolic perturbations induced by AgNPs on phytoplankton, essential organisms in global biogeochemical cycles...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wei Liu, Sanghamitra Majumdar, Weiwei Li, Arturo A. Keller, Vera I. Slaveykova
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2a41aa29d00c4fbea697e9a7855ab68f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2a41aa29d00c4fbea697e9a7855ab68f
record_format	dspace
spelling	oai:doaj.org-article:2a41aa29d00c4fbea697e9a7855ab68f2021-12-02T15:09:23ZMetabolomics for early detection of stress in freshwater alga Poterioochromonas malhamensis exposed to silver nanoparticles10.1038/s41598-020-77521-02045-2322https://doaj.org/article/2a41aa29d00c4fbea697e9a7855ab68f2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77521-0https://doaj.org/toc/2045-2322Abstract Silver nanoparticles (AgNPs) are one of the most used engineered nanomaterials. Despite progress in assessing their environmental implications, knowledge gaps exist concerning the metabolic perturbations induced by AgNPs on phytoplankton, essential organisms in global biogeochemical cycles and food-web dynamics. We combine targeted metabolomics, biouptake and physiological response studies to elucidate metabolic perturbations in alga Poterioochromonas malhamensis induced by AgNPs and dissolved Ag. We show time-dependent perturbation of the metabolism of amino acids, nucleotides, fatty acids, tricarboxylic acids, photosynthesis and photorespiration by both Ag-treatments. The results suggest that dissolved Ag ions released by AgNPs are the major toxicity driver; however, AgNPs internalized in food vacuoles contributed to the perturbation of amino acid metabolism, TCA cycle and oxidative stress. The metabolic perturbations corroborate the observed physiological responses. We highlight the potential of metabolomics as a tool for understanding the molecular basis for these metabolic and physiological changes, and for early detection of stress.Wei LiuSanghamitra MajumdarWeiwei LiArturo A. KellerVera I. SlaveykovaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei Liu Sanghamitra Majumdar Weiwei Li Arturo A. Keller Vera I. Slaveykova Metabolomics for early detection of stress in freshwater alga Poterioochromonas malhamensis exposed to silver nanoparticles
description	Abstract Silver nanoparticles (AgNPs) are one of the most used engineered nanomaterials. Despite progress in assessing their environmental implications, knowledge gaps exist concerning the metabolic perturbations induced by AgNPs on phytoplankton, essential organisms in global biogeochemical cycles and food-web dynamics. We combine targeted metabolomics, biouptake and physiological response studies to elucidate metabolic perturbations in alga Poterioochromonas malhamensis induced by AgNPs and dissolved Ag. We show time-dependent perturbation of the metabolism of amino acids, nucleotides, fatty acids, tricarboxylic acids, photosynthesis and photorespiration by both Ag-treatments. The results suggest that dissolved Ag ions released by AgNPs are the major toxicity driver; however, AgNPs internalized in food vacuoles contributed to the perturbation of amino acid metabolism, TCA cycle and oxidative stress. The metabolic perturbations corroborate the observed physiological responses. We highlight the potential of metabolomics as a tool for understanding the molecular basis for these metabolic and physiological changes, and for early detection of stress.
format	article
author	Wei Liu Sanghamitra Majumdar Weiwei Li Arturo A. Keller Vera I. Slaveykova
author_facet	Wei Liu Sanghamitra Majumdar Weiwei Li Arturo A. Keller Vera I. Slaveykova
author_sort	Wei Liu
title	Metabolomics for early detection of stress in freshwater alga Poterioochromonas malhamensis exposed to silver nanoparticles
title_short	Metabolomics for early detection of stress in freshwater alga Poterioochromonas malhamensis exposed to silver nanoparticles
title_full	Metabolomics for early detection of stress in freshwater alga Poterioochromonas malhamensis exposed to silver nanoparticles
title_fullStr	Metabolomics for early detection of stress in freshwater alga Poterioochromonas malhamensis exposed to silver nanoparticles
title_full_unstemmed	Metabolomics for early detection of stress in freshwater alga Poterioochromonas malhamensis exposed to silver nanoparticles
title_sort	metabolomics for early detection of stress in freshwater alga poterioochromonas malhamensis exposed to silver nanoparticles
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/2a41aa29d00c4fbea697e9a7855ab68f
work_keys_str_mv	AT weiliu metabolomicsforearlydetectionofstressinfreshwateralgapoterioochromonasmalhamensisexposedtosilvernanoparticles AT sanghamitramajumdar metabolomicsforearlydetectionofstressinfreshwateralgapoterioochromonasmalhamensisexposedtosilvernanoparticles AT weiweili metabolomicsforearlydetectionofstressinfreshwateralgapoterioochromonasmalhamensisexposedtosilvernanoparticles AT arturoakeller metabolomicsforearlydetectionofstressinfreshwateralgapoterioochromonasmalhamensisexposedtosilvernanoparticles AT veraislaveykova metabolomicsforearlydetectionofstressinfreshwateralgapoterioochromonasmalhamensisexposedtosilvernanoparticles
_version_	1718387802467467264

Metabolomics for early detection of stress in freshwater alga Poterioochromonas malhamensis exposed to silver nanoparticles

Ejemplares similares