Spermine-Mediated Tolerance to Selenium Toxicity in Wheat (<i>Triticum aestivum</i> L.) Depends on Endogenous Nitric Oxide Synthesis

Excess selenium (Se) causes toxicity, and nitric oxide (NO)’s function in spermine (Spm)-induced tolerance to Se stress is unknown. Using wheat plants exposed to 1 mM sodium selenate—alone or in combination with either 1 mM Spm, 0.1 mM NO donor sodium nitroprusside (SNP) or 0.1 mM NO scavenger cPTIO...

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Autores principales:	Md. Mahadi Hasan, Basmah M. Alharbi, Haifa Abdulaziz Sakit Alhaithloul, Awatif M. Abdulmajeed, Suliman Mohammed Alghanem, Amina A. M. Al-Mushhin, Mohammad Shah Jahan, Francisco J. Corpas, Xiang-Wen Fang, Mona H. Soliman
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	antioxidant enzymes gene expression glyoxalase systems oxidative stress reactive oxygen species (ROS) Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/2a48af8483404b0eb6bb9641d1b3c6ef
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spelling	oai:doaj.org-article:2a48af8483404b0eb6bb9641d1b3c6ef2021-11-25T16:29:33ZSpermine-Mediated Tolerance to Selenium Toxicity in Wheat (<i>Triticum aestivum</i> L.) Depends on Endogenous Nitric Oxide Synthesis10.3390/antiox101118352076-3921https://doaj.org/article/2a48af8483404b0eb6bb9641d1b3c6ef2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1835https://doaj.org/toc/2076-3921Excess selenium (Se) causes toxicity, and nitric oxide (NO)’s function in spermine (Spm)-induced tolerance to Se stress is unknown. Using wheat plants exposed to 1 mM sodium selenate—alone or in combination with either 1 mM Spm, 0.1 mM NO donor sodium nitroprusside (SNP) or 0.1 mM NO scavenger cPTIO—the potential beneficial effects of these compounds to palliate Se-induced stress were evaluated at physiological, biochemical and molecular levels. Se-treated plants accumulated Se in their roots (92%) and leaves (95%) more than control plants. Furthermore, Se diminished plant growth, photosynthetic traits and the relative water content and increased the levels of malondialdehyde, H<sub>2</sub>O<sub>2</sub>, osmolyte and endogenous NO. Exogenous Spm significantly decreased the levels of malondialdehyde by 28%, H<sub>2</sub>O<sub>2</sub> by 37% and electrolyte leakage by 42%. Combined Spm/NO treatment reduced the Se content and triggered plant growth, photosynthetic traits, antioxidant enzymes and glyoxalase systems. Spm/NO also upregulated <i>MTP1</i>, <i>MTPC3</i> and <i>HSP70</i> and downregulated <i>TaPCS1</i> and <i>NRAMP1</i> (metal stress-related genes involved in selenium uptake, translocation and detoxification). However, the positive effects of Spm on Se-stressed plants were eliminated by the NO scavenger. Accordingly, data support the notion that Spm palliates selenium-induced oxidative stress since the induced NO elicits antioxidant defence upregulation but downregulates Se uptake and translocation. These findings pave the way for potential biotechnological approaches to supporting sustainable wheat crop production in selenium-contaminated areas.Md. Mahadi HasanBasmah M. AlharbiHaifa Abdulaziz Sakit AlhaithloulAwatif M. AbdulmajeedSuliman Mohammed AlghanemAmina A. M. Al-MushhinMohammad Shah JahanFrancisco J. CorpasXiang-Wen FangMona H. SolimanMDPI AGarticleantioxidant enzymesgene expressionglyoxalase systemsoxidative stressreactive oxygen species (ROS)Therapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1835, p 1835 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	antioxidant enzymes gene expression glyoxalase systems oxidative stress reactive oxygen species (ROS) Therapeutics. Pharmacology RM1-950
spellingShingle	antioxidant enzymes gene expression glyoxalase systems oxidative stress reactive oxygen species (ROS) Therapeutics. Pharmacology RM1-950 Md. Mahadi Hasan Basmah M. Alharbi Haifa Abdulaziz Sakit Alhaithloul Awatif M. Abdulmajeed Suliman Mohammed Alghanem Amina A. M. Al-Mushhin Mohammad Shah Jahan Francisco J. Corpas Xiang-Wen Fang Mona H. Soliman Spermine-Mediated Tolerance to Selenium Toxicity in Wheat (<i>Triticum aestivum</i> L.) Depends on Endogenous Nitric Oxide Synthesis
description	Excess selenium (Se) causes toxicity, and nitric oxide (NO)’s function in spermine (Spm)-induced tolerance to Se stress is unknown. Using wheat plants exposed to 1 mM sodium selenate—alone or in combination with either 1 mM Spm, 0.1 mM NO donor sodium nitroprusside (SNP) or 0.1 mM NO scavenger cPTIO—the potential beneficial effects of these compounds to palliate Se-induced stress were evaluated at physiological, biochemical and molecular levels. Se-treated plants accumulated Se in their roots (92%) and leaves (95%) more than control plants. Furthermore, Se diminished plant growth, photosynthetic traits and the relative water content and increased the levels of malondialdehyde, H<sub>2</sub>O<sub>2</sub>, osmolyte and endogenous NO. Exogenous Spm significantly decreased the levels of malondialdehyde by 28%, H<sub>2</sub>O<sub>2</sub> by 37% and electrolyte leakage by 42%. Combined Spm/NO treatment reduced the Se content and triggered plant growth, photosynthetic traits, antioxidant enzymes and glyoxalase systems. Spm/NO also upregulated <i>MTP1</i>, <i>MTPC3</i> and <i>HSP70</i> and downregulated <i>TaPCS1</i> and <i>NRAMP1</i> (metal stress-related genes involved in selenium uptake, translocation and detoxification). However, the positive effects of Spm on Se-stressed plants were eliminated by the NO scavenger. Accordingly, data support the notion that Spm palliates selenium-induced oxidative stress since the induced NO elicits antioxidant defence upregulation but downregulates Se uptake and translocation. These findings pave the way for potential biotechnological approaches to supporting sustainable wheat crop production in selenium-contaminated areas.
format	article
author	Md. Mahadi Hasan Basmah M. Alharbi Haifa Abdulaziz Sakit Alhaithloul Awatif M. Abdulmajeed Suliman Mohammed Alghanem Amina A. M. Al-Mushhin Mohammad Shah Jahan Francisco J. Corpas Xiang-Wen Fang Mona H. Soliman
author_facet	Md. Mahadi Hasan Basmah M. Alharbi Haifa Abdulaziz Sakit Alhaithloul Awatif M. Abdulmajeed Suliman Mohammed Alghanem Amina A. M. Al-Mushhin Mohammad Shah Jahan Francisco J. Corpas Xiang-Wen Fang Mona H. Soliman
author_sort	Md. Mahadi Hasan
title	Spermine-Mediated Tolerance to Selenium Toxicity in Wheat (<i>Triticum aestivum</i> L.) Depends on Endogenous Nitric Oxide Synthesis
title_short	Spermine-Mediated Tolerance to Selenium Toxicity in Wheat (<i>Triticum aestivum</i> L.) Depends on Endogenous Nitric Oxide Synthesis
title_full	Spermine-Mediated Tolerance to Selenium Toxicity in Wheat (<i>Triticum aestivum</i> L.) Depends on Endogenous Nitric Oxide Synthesis
title_fullStr	Spermine-Mediated Tolerance to Selenium Toxicity in Wheat (<i>Triticum aestivum</i> L.) Depends on Endogenous Nitric Oxide Synthesis
title_full_unstemmed	Spermine-Mediated Tolerance to Selenium Toxicity in Wheat (<i>Triticum aestivum</i> L.) Depends on Endogenous Nitric Oxide Synthesis
title_sort	spermine-mediated tolerance to selenium toxicity in wheat (<i>triticum aestivum</i> l.) depends on endogenous nitric oxide synthesis
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2a48af8483404b0eb6bb9641d1b3c6ef
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Spermine-Mediated Tolerance to Selenium Toxicity in Wheat (<i>Triticum aestivum</i> L.) Depends on Endogenous Nitric Oxide Synthesis

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