Protocol for analysis of mouse neutrophil NETosis by flow cytometry

Summary: Studies involving neutrophils are steadily increasing, thus creating a need for more optimized and thorough protocols for studying neutrophil function. Here, we present our protocol for extracting mouse bone marrow neutrophils, estimating the purity of isolated neutrophils, and assessing th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cassandra J. McGill, Ryan J. Lu, Bérénice A. Benayoun
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell isolation Flow Cytometry/Mass Cytometry Cell-based Assays Immunology Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/2a4c036c26764bc6b957bc32de39b994
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2a4c036c26764bc6b957bc32de39b994
record_format	dspace
spelling	oai:doaj.org-article:2a4c036c26764bc6b957bc32de39b9942021-11-14T04:35:37ZProtocol for analysis of mouse neutrophil NETosis by flow cytometry2666-166710.1016/j.xpro.2021.100948https://doaj.org/article/2a4c036c26764bc6b957bc32de39b9942021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006547https://doaj.org/toc/2666-1667Summary: Studies involving neutrophils are steadily increasing, thus creating a need for more optimized and thorough protocols for studying neutrophil function. Here, we present our protocol for extracting mouse bone marrow neutrophils, estimating the purity of isolated neutrophils, and assessing their ability to induce NETosis upon an external cue. We test two isolation protocols that can be used to attain neutrophils to assess NETosis induction. This approach allows for the parallel assessment of NETosis induction in cohorts larger than 10 samples.For complete details on the use and execution of this protocol, please refer to Lu et al., 2021.Cassandra J. McGillRyan J. LuBérénice A. BenayounElsevierarticleCell isolationFlow Cytometry/Mass CytometryCell-based AssaysImmunologyScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100948- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell isolation Flow Cytometry/Mass Cytometry Cell-based Assays Immunology Science (General) Q1-390
spellingShingle	Cell isolation Flow Cytometry/Mass Cytometry Cell-based Assays Immunology Science (General) Q1-390 Cassandra J. McGill Ryan J. Lu Bérénice A. Benayoun Protocol for analysis of mouse neutrophil NETosis by flow cytometry
description	Summary: Studies involving neutrophils are steadily increasing, thus creating a need for more optimized and thorough protocols for studying neutrophil function. Here, we present our protocol for extracting mouse bone marrow neutrophils, estimating the purity of isolated neutrophils, and assessing their ability to induce NETosis upon an external cue. We test two isolation protocols that can be used to attain neutrophils to assess NETosis induction. This approach allows for the parallel assessment of NETosis induction in cohorts larger than 10 samples.For complete details on the use and execution of this protocol, please refer to Lu et al., 2021.
format	article
author	Cassandra J. McGill Ryan J. Lu Bérénice A. Benayoun
author_facet	Cassandra J. McGill Ryan J. Lu Bérénice A. Benayoun
author_sort	Cassandra J. McGill
title	Protocol for analysis of mouse neutrophil NETosis by flow cytometry
title_short	Protocol for analysis of mouse neutrophil NETosis by flow cytometry
title_full	Protocol for analysis of mouse neutrophil NETosis by flow cytometry
title_fullStr	Protocol for analysis of mouse neutrophil NETosis by flow cytometry
title_full_unstemmed	Protocol for analysis of mouse neutrophil NETosis by flow cytometry
title_sort	protocol for analysis of mouse neutrophil netosis by flow cytometry
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2a4c036c26764bc6b957bc32de39b994
work_keys_str_mv	AT cassandrajmcgill protocolforanalysisofmouseneutrophilnetosisbyflowcytometry AT ryanjlu protocolforanalysisofmouseneutrophilnetosisbyflowcytometry AT bereniceabenayoun protocolforanalysisofmouseneutrophilnetosisbyflowcytometry
_version_	1718429886835589120

Protocol for analysis of mouse neutrophil NETosis by flow cytometry

Ejemplares similares