Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections

Commensal fungi are an important part of human microbial community, among which Candida albicans and Candida glabrata are two common opportunistic pathogens. Unlike the high pathogenicity of C. albicans, C. glabrata is reported to show low pathogenicity to the host. Here, by using a Galleria mellone...

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Autores principales:	Xiao-Wen Huang, Mei-Nian Xu, Huan-Xin Zheng, Meng-Lei Wang, Li Li, Kang Zeng, De-Dong Li
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	candida glabrata galleria mellonella innate immunity hemocytes immune priming Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/2a4d6e29faac4331b3123d977e752a5b
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spelling	oai:doaj.org-article:2a4d6e29faac4331b3123d977e752a5b2021-11-17T14:21:59ZPre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections2150-55942150-560810.1080/21505594.2020.1848107https://doaj.org/article/2a4d6e29faac4331b3123d977e752a5b2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1848107https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Commensal fungi are an important part of human microbial community, among which Candida albicans and Candida glabrata are two common opportunistic pathogens. Unlike the high pathogenicity of C. albicans, C. glabrata is reported to show low pathogenicity to the host. Here, by using a Galleria mellonella infection model, we were able to confirm the much lower virulence of C. glabrata than C. albicans. Interestingly, pre-exposure to live C. glabrata (LCG) protects the larvae against subsequent various lethal fungal infections, including C. albicans, Candida tropicalis, and Cryptococcus neoformans. Inconsistently, heat-inactivated C. glabrata (HICG) pre-exposure can only protect against C. albicans or C. tropicalis re-infection, but not C. neoformans. Mechanistically, LCG or HICG pre-exposure enhanced the fungicidal activity of hemocytes against C. albicans or C. tropicalis. Meanwhile, LCG pre-exposure enhanced the humoral immunity by modulating the expression of fungal defending proteins in the cell-free hemolymph, which may contribute to the protection against C. neoformans. Together, this study suggests the important role of C. glabrata in enhancing host immunity, and demonstrates the great potential of G. mellonella model in studying the innate immune responses against infections.Xiao-Wen HuangMei-Nian XuHuan-Xin ZhengMeng-Lei WangLi LiKang ZengDe-Dong LiTaylor & Francis Grouparticlecandida glabratagalleria mellonellainnate immunityhemocytesimmune primingInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1674-1684 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	candida glabrata galleria mellonella innate immunity hemocytes immune priming Infectious and parasitic diseases RC109-216
spellingShingle	candida glabrata galleria mellonella innate immunity hemocytes immune priming Infectious and parasitic diseases RC109-216 Xiao-Wen Huang Mei-Nian Xu Huan-Xin Zheng Meng-Lei Wang Li Li Kang Zeng De-Dong Li Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections
description	Commensal fungi are an important part of human microbial community, among which Candida albicans and Candida glabrata are two common opportunistic pathogens. Unlike the high pathogenicity of C. albicans, C. glabrata is reported to show low pathogenicity to the host. Here, by using a Galleria mellonella infection model, we were able to confirm the much lower virulence of C. glabrata than C. albicans. Interestingly, pre-exposure to live C. glabrata (LCG) protects the larvae against subsequent various lethal fungal infections, including C. albicans, Candida tropicalis, and Cryptococcus neoformans. Inconsistently, heat-inactivated C. glabrata (HICG) pre-exposure can only protect against C. albicans or C. tropicalis re-infection, but not C. neoformans. Mechanistically, LCG or HICG pre-exposure enhanced the fungicidal activity of hemocytes against C. albicans or C. tropicalis. Meanwhile, LCG pre-exposure enhanced the humoral immunity by modulating the expression of fungal defending proteins in the cell-free hemolymph, which may contribute to the protection against C. neoformans. Together, this study suggests the important role of C. glabrata in enhancing host immunity, and demonstrates the great potential of G. mellonella model in studying the innate immune responses against infections.
format	article
author	Xiao-Wen Huang Mei-Nian Xu Huan-Xin Zheng Meng-Lei Wang Li Li Kang Zeng De-Dong Li
author_facet	Xiao-Wen Huang Mei-Nian Xu Huan-Xin Zheng Meng-Lei Wang Li Li Kang Zeng De-Dong Li
author_sort	Xiao-Wen Huang
title	Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections
title_short	Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections
title_full	Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections
title_fullStr	Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections
title_full_unstemmed	Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections
title_sort	pre-exposure to candida glabrata protects galleria mellonella against subsequent lethal fungal infections
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/2a4d6e29faac4331b3123d977e752a5b
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Pre-exposure to Candida glabrata protects Galleria mellonella against subsequent lethal fungal infections

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