Assessing Plasma Levels of α-Synuclein and Neurofilament Light Chain by Different Blood Preparation Methods

The potential biomarkers of Parkinson’s disease are α-synuclein and neurofilament light chain (NFL). However, inconsistent preanalytical preparation of plasma could lead to variations in levels of these biomarkers. Different types of potassium salts of EDTA and different centrifugation temperatures...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kuo-Hsuan Chang, Kou-Chen Liu, Chao-Sung Lai, Shieh-Yueh Yang, Chiung-Mei Chen
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Parkinson’s disease biomarker α-synuclein neurofilament light chain ethylenediaminetetraacetic acid centrifugation temperature Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/2a504dff10e14421a7fd166ed0485963
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2a504dff10e14421a7fd166ed0485963
record_format	dspace
spelling	oai:doaj.org-article:2a504dff10e14421a7fd166ed04859632021-11-08T16:56:54ZAssessing Plasma Levels of α-Synuclein and Neurofilament Light Chain by Different Blood Preparation Methods1663-436510.3389/fnagi.2021.759182https://doaj.org/article/2a504dff10e14421a7fd166ed04859632021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnagi.2021.759182/fullhttps://doaj.org/toc/1663-4365The potential biomarkers of Parkinson’s disease are α-synuclein and neurofilament light chain (NFL). However, inconsistent preanalytical preparation of plasma could lead to variations in levels of these biomarkers. Different types of potassium salts of EDTA and different centrifugation temperatures during plasma preparation may affect the results of α-synuclein and NFL measurements. In this study, we prepared plasma from eight patients with Parkinson’s disease (PD) and seven healthy controls (HCs) by using di- and tri-potassium (K2- and K3-) EDTA tubes and recruited a separated cohort with 42 PD patients and 40 HCs for plasma samples prepared from whole blood by centrifugation at room temperature and 4°C, respectively, in K2-EDTA tubes. The plasma levels of α-synuclein and NFL in K2- and K3-EDTA were similar. However, the levels of α-synuclein in the plasma prepared at 4°C (101.57 ± 43.43 fg/ml) were significantly lower compared with those at room temperature (181.23 ± 196.31 fg/ml, P < 0.001). Room temperature preparation demonstrated elevated plasma levels of α-synuclein in PD patients (256.6 ± 50.2 fg/ml) compared with the HCs (102.1 ± 0.66 fg/ml, P < 0.001), whereas this increase in PD was not present by preparation at 4°C. Both plasma preparations at room temperature and 4°C demonstrated consistent results of NFL, which are increased in PD patients compared with HCs. Our findings confirmed that K2- and K3-EDTA tubes were interchangeable for analyzing plasma levels of α-synuclein and NFL. Centrifugation at 4°C during plasma preparation generates considerable reduction and variation of α-synuclein level that might hinder the detection of α-synuclein level changes in PD.Kuo-Hsuan ChangKou-Chen LiuKou-Chen LiuChao-Sung LaiChao-Sung LaiChao-Sung LaiShieh-Yueh YangChiung-Mei ChenFrontiers Media S.A.articleParkinson’s diseasebiomarkerα-synucleinneurofilament light chainethylenediaminetetraacetic acidcentrifugation temperatureNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Aging Neuroscience, Vol 13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Parkinson’s disease biomarker α-synuclein neurofilament light chain ethylenediaminetetraacetic acid centrifugation temperature Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	Parkinson’s disease biomarker α-synuclein neurofilament light chain ethylenediaminetetraacetic acid centrifugation temperature Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Kuo-Hsuan Chang Kou-Chen Liu Kou-Chen Liu Chao-Sung Lai Chao-Sung Lai Chao-Sung Lai Shieh-Yueh Yang Chiung-Mei Chen Assessing Plasma Levels of α-Synuclein and Neurofilament Light Chain by Different Blood Preparation Methods
description	The potential biomarkers of Parkinson’s disease are α-synuclein and neurofilament light chain (NFL). However, inconsistent preanalytical preparation of plasma could lead to variations in levels of these biomarkers. Different types of potassium salts of EDTA and different centrifugation temperatures during plasma preparation may affect the results of α-synuclein and NFL measurements. In this study, we prepared plasma from eight patients with Parkinson’s disease (PD) and seven healthy controls (HCs) by using di- and tri-potassium (K2- and K3-) EDTA tubes and recruited a separated cohort with 42 PD patients and 40 HCs for plasma samples prepared from whole blood by centrifugation at room temperature and 4°C, respectively, in K2-EDTA tubes. The plasma levels of α-synuclein and NFL in K2- and K3-EDTA were similar. However, the levels of α-synuclein in the plasma prepared at 4°C (101.57 ± 43.43 fg/ml) were significantly lower compared with those at room temperature (181.23 ± 196.31 fg/ml, P < 0.001). Room temperature preparation demonstrated elevated plasma levels of α-synuclein in PD patients (256.6 ± 50.2 fg/ml) compared with the HCs (102.1 ± 0.66 fg/ml, P < 0.001), whereas this increase in PD was not present by preparation at 4°C. Both plasma preparations at room temperature and 4°C demonstrated consistent results of NFL, which are increased in PD patients compared with HCs. Our findings confirmed that K2- and K3-EDTA tubes were interchangeable for analyzing plasma levels of α-synuclein and NFL. Centrifugation at 4°C during plasma preparation generates considerable reduction and variation of α-synuclein level that might hinder the detection of α-synuclein level changes in PD.
format	article
author	Kuo-Hsuan Chang Kou-Chen Liu Kou-Chen Liu Chao-Sung Lai Chao-Sung Lai Chao-Sung Lai Shieh-Yueh Yang Chiung-Mei Chen
author_facet	Kuo-Hsuan Chang Kou-Chen Liu Kou-Chen Liu Chao-Sung Lai Chao-Sung Lai Chao-Sung Lai Shieh-Yueh Yang Chiung-Mei Chen
author_sort	Kuo-Hsuan Chang
title	Assessing Plasma Levels of α-Synuclein and Neurofilament Light Chain by Different Blood Preparation Methods
title_short	Assessing Plasma Levels of α-Synuclein and Neurofilament Light Chain by Different Blood Preparation Methods
title_full	Assessing Plasma Levels of α-Synuclein and Neurofilament Light Chain by Different Blood Preparation Methods
title_fullStr	Assessing Plasma Levels of α-Synuclein and Neurofilament Light Chain by Different Blood Preparation Methods
title_full_unstemmed	Assessing Plasma Levels of α-Synuclein and Neurofilament Light Chain by Different Blood Preparation Methods
title_sort	assessing plasma levels of α-synuclein and neurofilament light chain by different blood preparation methods
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2a504dff10e14421a7fd166ed0485963
work_keys_str_mv	AT kuohsuanchang assessingplasmalevelsofasynucleinandneurofilamentlightchainbydifferentbloodpreparationmethods AT kouchenliu assessingplasmalevelsofasynucleinandneurofilamentlightchainbydifferentbloodpreparationmethods AT kouchenliu assessingplasmalevelsofasynucleinandneurofilamentlightchainbydifferentbloodpreparationmethods AT chaosunglai assessingplasmalevelsofasynucleinandneurofilamentlightchainbydifferentbloodpreparationmethods AT chaosunglai assessingplasmalevelsofasynucleinandneurofilamentlightchainbydifferentbloodpreparationmethods AT chaosunglai assessingplasmalevelsofasynucleinandneurofilamentlightchainbydifferentbloodpreparationmethods AT shiehyuehyang assessingplasmalevelsofasynucleinandneurofilamentlightchainbydifferentbloodpreparationmethods AT chiungmeichen assessingplasmalevelsofasynucleinandneurofilamentlightchainbydifferentbloodpreparationmethods
_version_	1718441456509648896

Assessing Plasma Levels of α-Synuclein and Neurofilament Light Chain by Different Blood Preparation Methods

Ejemplares similares