PGMD/curcumin nanoparticles for the treatment of breast cancer

Abstract The present study aims at developing PGMD (poly-glycerol-malic acid-dodecanedioic acid)/curcumin nanoparticles based formulation for anticancer activity against breast cancer cells. The nanoparticles were prepared using both the variants of PGMD polymer (PGMD 7:3 and PGMD 6:4) with curcumin...

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Autores principales:	Mankamna Kumari, Nikita Sharma, Romila Manchanda, Nidhi Gupta, Asad Syed, Ali H. Bahkali, Surendra Nimesh
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2a5e5347587949e0baab8905d118dcc3
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spelling	oai:doaj.org-article:2a5e5347587949e0baab8905d118dcc32021-12-02T12:11:07ZPGMD/curcumin nanoparticles for the treatment of breast cancer10.1038/s41598-021-81701-x2045-2322https://doaj.org/article/2a5e5347587949e0baab8905d118dcc32021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81701-xhttps://doaj.org/toc/2045-2322Abstract The present study aims at developing PGMD (poly-glycerol-malic acid-dodecanedioic acid)/curcumin nanoparticles based formulation for anticancer activity against breast cancer cells. The nanoparticles were prepared using both the variants of PGMD polymer (PGMD 7:3 and PGMD 6:4) with curcumin (i.e. CUR NP 7:3 and CUR NP 6:4). The size of CUR NP 7:3 and CUR NP 6:4 were found to be ~ 110 and 218 nm with a polydispersity index of 0.174 and 0.36, respectively. Further, the zeta potential of the particles was − 18.9 and − 17.5 mV for CUR NP 7:3 and CUR NP 6:4, respectively. The entrapment efficiency of both the nanoparticles was in the range of 75–81%. In vitro anticancer activity and the scratch assay were conducted on breast cancer cell lines, MCF-7 and MDA-MB-231. The IC50 of the nanoformulations was observed to be 40.2 and 33.6 μM at 48 h for CUR NP 7:3 and CUR NP 6:4, respectively, in MCF-7 cell line; for MDA-MB-231 it was 43.4 and 30.5 μM. Acridine orange/EtBr and DAPI staining assays showed apoptotic features and nuclear anomalies in the treated cells. This was further confirmed by western blot analysis that showed overexpression of caspase 9 indicating curcumin role in apoptosis.Mankamna KumariNikita SharmaRomila ManchandaNidhi GuptaAsad SyedAli H. BahkaliSurendra NimeshNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mankamna Kumari Nikita Sharma Romila Manchanda Nidhi Gupta Asad Syed Ali H. Bahkali Surendra Nimesh PGMD/curcumin nanoparticles for the treatment of breast cancer
description	Abstract The present study aims at developing PGMD (poly-glycerol-malic acid-dodecanedioic acid)/curcumin nanoparticles based formulation for anticancer activity against breast cancer cells. The nanoparticles were prepared using both the variants of PGMD polymer (PGMD 7:3 and PGMD 6:4) with curcumin (i.e. CUR NP 7:3 and CUR NP 6:4). The size of CUR NP 7:3 and CUR NP 6:4 were found to be ~ 110 and 218 nm with a polydispersity index of 0.174 and 0.36, respectively. Further, the zeta potential of the particles was − 18.9 and − 17.5 mV for CUR NP 7:3 and CUR NP 6:4, respectively. The entrapment efficiency of both the nanoparticles was in the range of 75–81%. In vitro anticancer activity and the scratch assay were conducted on breast cancer cell lines, MCF-7 and MDA-MB-231. The IC50 of the nanoformulations was observed to be 40.2 and 33.6 μM at 48 h for CUR NP 7:3 and CUR NP 6:4, respectively, in MCF-7 cell line; for MDA-MB-231 it was 43.4 and 30.5 μM. Acridine orange/EtBr and DAPI staining assays showed apoptotic features and nuclear anomalies in the treated cells. This was further confirmed by western blot analysis that showed overexpression of caspase 9 indicating curcumin role in apoptosis.
format	article
author	Mankamna Kumari Nikita Sharma Romila Manchanda Nidhi Gupta Asad Syed Ali H. Bahkali Surendra Nimesh
author_facet	Mankamna Kumari Nikita Sharma Romila Manchanda Nidhi Gupta Asad Syed Ali H. Bahkali Surendra Nimesh
author_sort	Mankamna Kumari
title	PGMD/curcumin nanoparticles for the treatment of breast cancer
title_short	PGMD/curcumin nanoparticles for the treatment of breast cancer
title_full	PGMD/curcumin nanoparticles for the treatment of breast cancer
title_fullStr	PGMD/curcumin nanoparticles for the treatment of breast cancer
title_full_unstemmed	PGMD/curcumin nanoparticles for the treatment of breast cancer
title_sort	pgmd/curcumin nanoparticles for the treatment of breast cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2a5e5347587949e0baab8905d118dcc3
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PGMD/curcumin nanoparticles for the treatment of breast cancer

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