Identification of Malassezia species as part of normal skin and ear canal microbiota in horses

Background: The yeasts of the genus Malassezia are considered part of the normal skin microbiota in humans and animals. In horses, several species of the genus Malassezia have been reported in different areas of the skin and ear canal. Objective: Isolate, characterize and identify the different spec...

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Autores principales:	Rubiela Castañeda Salazar, Diana M. Rodríguez Sandoval, Adriana P. Pulido-Villamarín, Melva Y. Linares Linares, Olimpo J. Oliver Espinosa
Formato:	article
Lenguaje:	EN
Publicado:	Universidad de Antioquia 2020
Materias:	culture ear canal horses identification malassezia normal microbiota pcr skin yeast Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/2a604a541df04a79b0920d07f9b768d9
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spelling	oai:doaj.org-article:2a604a541df04a79b0920d07f9b768d92021-12-01T15:44:00ZIdentification of Malassezia species as part of normal skin and ear canal microbiota in horses2256-295810.17533/udea.rccp.v33n1a01https://doaj.org/article/2a604a541df04a79b0920d07f9b768d92020-01-01T00:00:00Zhttps://revistas.udea.edu.co/index.php/rccp/article/view/338224https://doaj.org/toc/2256-2958Background: The yeasts of the genus Malassezia are considered part of the normal skin microbiota in humans and animals. In horses, several species of the genus Malassezia have been reported in different areas of the skin and ear canal. Objective: Isolate, characterize and identify the different species belonging to the genus Malassezia isolated from the ear canal and skin of equine patients with no dermatological lesions that were referred to the large animal clinic of veterinary teaching hospital at the National University of Colombia. Methods: 22 horses were evaluated and sampled. Eighty-two samples were obtained by swabbing either the ear canals (left and right), skin areas of prepuce, mammary gland and inguinal region. The samples were examined by cytological evaluation and were cultured on modified Dixon’s agar and phenotypic and molecular identification were performed for yeast colonies. Results: Fourteen yeast isolates were obtained from the 82 samples. Biochemical identification determined that 50% (n=7) were Malassezia spp., 35.7% (n=5) were identified as Candida spp. and 14.3% (n=2) as Cryptococcus spp. Using molecular tests, the Malassezia species were M. slooffiae(28.6%) and M. nana (57.1%); only one isolate was classified as Trichosporo asahii. Conclusion: M. nana and M. slooffiae were identified as part of the normal ear canal and skin microbiota in the evaluated horses. The observed prevalence of Malassezia spp. was 18.2% (n=4/22) in this study sample.Rubiela Castañeda SalazarDiana M. Rodríguez SandovalAdriana P. Pulido-VillamarínMelva Y. Linares LinaresOlimpo J. Oliver EspinosaUniversidad de Antioquiaarticlecultureear canalhorsesidentificationmalassezianormal microbiotapcrskinyeastAnimal cultureSF1-1100ENRevista Colombiana de Ciencias Pecuarias, Vol 33, Iss 1, Pp 5-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	culture ear canal horses identification malassezia normal microbiota pcr skin yeast Animal culture SF1-1100
spellingShingle	culture ear canal horses identification malassezia normal microbiota pcr skin yeast Animal culture SF1-1100 Rubiela Castañeda Salazar Diana M. Rodríguez Sandoval Adriana P. Pulido-Villamarín Melva Y. Linares Linares Olimpo J. Oliver Espinosa Identification of Malassezia species as part of normal skin and ear canal microbiota in horses
description	Background: The yeasts of the genus Malassezia are considered part of the normal skin microbiota in humans and animals. In horses, several species of the genus Malassezia have been reported in different areas of the skin and ear canal. Objective: Isolate, characterize and identify the different species belonging to the genus Malassezia isolated from the ear canal and skin of equine patients with no dermatological lesions that were referred to the large animal clinic of veterinary teaching hospital at the National University of Colombia. Methods: 22 horses were evaluated and sampled. Eighty-two samples were obtained by swabbing either the ear canals (left and right), skin areas of prepuce, mammary gland and inguinal region. The samples were examined by cytological evaluation and were cultured on modified Dixon’s agar and phenotypic and molecular identification were performed for yeast colonies. Results: Fourteen yeast isolates were obtained from the 82 samples. Biochemical identification determined that 50% (n=7) were Malassezia spp., 35.7% (n=5) were identified as Candida spp. and 14.3% (n=2) as Cryptococcus spp. Using molecular tests, the Malassezia species were M. slooffiae(28.6%) and M. nana (57.1%); only one isolate was classified as Trichosporo asahii. Conclusion: M. nana and M. slooffiae were identified as part of the normal ear canal and skin microbiota in the evaluated horses. The observed prevalence of Malassezia spp. was 18.2% (n=4/22) in this study sample.
format	article
author	Rubiela Castañeda Salazar Diana M. Rodríguez Sandoval Adriana P. Pulido-Villamarín Melva Y. Linares Linares Olimpo J. Oliver Espinosa
author_facet	Rubiela Castañeda Salazar Diana M. Rodríguez Sandoval Adriana P. Pulido-Villamarín Melva Y. Linares Linares Olimpo J. Oliver Espinosa
author_sort	Rubiela Castañeda Salazar
title	Identification of Malassezia species as part of normal skin and ear canal microbiota in horses
title_short	Identification of Malassezia species as part of normal skin and ear canal microbiota in horses
title_full	Identification of Malassezia species as part of normal skin and ear canal microbiota in horses
title_fullStr	Identification of Malassezia species as part of normal skin and ear canal microbiota in horses
title_full_unstemmed	Identification of Malassezia species as part of normal skin and ear canal microbiota in horses
title_sort	identification of malassezia species as part of normal skin and ear canal microbiota in horses
publisher	Universidad de Antioquia
publishDate	2020
url	https://doaj.org/article/2a604a541df04a79b0920d07f9b768d9
work_keys_str_mv	AT rubielacastanedasalazar identificationofmalasseziaspeciesaspartofnormalskinandearcanalmicrobiotainhorses AT dianamrodriguezsandoval identificationofmalasseziaspeciesaspartofnormalskinandearcanalmicrobiotainhorses AT adrianappulidovillamarin identificationofmalasseziaspeciesaspartofnormalskinandearcanalmicrobiotainhorses AT melvaylinareslinares identificationofmalasseziaspeciesaspartofnormalskinandearcanalmicrobiotainhorses AT olimpojoliverespinosa identificationofmalasseziaspeciesaspartofnormalskinandearcanalmicrobiotainhorses
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Identification of Malassezia species as part of normal skin and ear canal microbiota in horses

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