In vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant Umbilicus rupestris

Abstract. Benhouda A, Benhouda D, Yahia M. 2019. In vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant Umbilicus rupestris. Biodiversitas 20: 3478-3483. Umbilicus rupestris (Crassulaceae) is a medicinal plant used in general traditional medicine to cure inflamma...

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Autores principales:	AFAF BENHOUDA, DJAHIDA BENHOUDA, MASSINISSA YAHIA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	algeria, cryptosporidium, umbilicus rupestris, immunofluorescence, mpo, oocysts Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2a647f7c4a804f65b45e24a9c72af572
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spelling	oai:doaj.org-article:2a647f7c4a804f65b45e24a9c72af5722021-11-21T21:59:05ZIn vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant Umbilicus rupestris1412-033X2085-472210.13057/biodiv/d201203https://doaj.org/article/2a647f7c4a804f65b45e24a9c72af5722019-11-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4678https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Benhouda A, Benhouda D, Yahia M. 2019. In vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant Umbilicus rupestris. Biodiversitas 20: 3478-3483. Umbilicus rupestris (Crassulaceae) is a medicinal plant used in general traditional medicine to cure inflammation and irritation of the skin. The present research is aimed to evaluate the antiparasitic activity of the methanolic extract of the plant URMeOH of U. rupestris against the Cryptosporidium infection in immunocompetent and immunosuppressed rats experimentally infected. Twenty-one female rats were divided into two groups. Control group (group I) and experimental group (Group II). The group I was further divided into three equal groups (normal group infected and immunosuppressed infected group). The experimental group was divided into two immunosuppressed and four equal groups and two immunocompetent infected. Cryptosporidium oocysts were isolated from bovine species stools and used to infect rats. Experimental subgroups received URMeOH two as dose 100mg/kg b.w. and 200 mg/kg b.w. and continued until 15 days. Two weeks after the administration of URMeOH, feces of rats were examined for the detection of Cryptosporidium oocysts by Ziehl-Neelsen and immunofluorescence techniques, the animals were sacrificed; their small intestines were processed and examined for the detection of pathological lesions after histopathological study. In addition, the activity of myeloperoxidase (MPO) was measured in sections of the jejunum. Concerned the results, we observed a statistically significant (P<0.001) increase in the number of oocysts of Cryptosporidium in the stool for sub infected immunosuppressed groups and an increase of MPO activity compared to the corresponding subgroups immunocompetent subgroups. The URMeOH could remove Cryptosporidium oocysts from feces and intestinal sections subgroup infected immunocompetent rats receiving URMeOH. Moreover, the oocysts were significantly reduced in all other subgroups experimental infected compared to infected control subgroups. Intestinal sections in all subgroups received URMeOH revealed a more or less normal architecture. In addition, the reduction of MPO activity level was also detected in all experimental subgroups.AFAF BENHOUDADJAHIDA BENHOUDAMASSINISSA YAHIAMBI & UNS Soloarticlealgeria, cryptosporidium, umbilicus rupestris, immunofluorescence, mpo, oocystsBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	algeria, cryptosporidium, umbilicus rupestris, immunofluorescence, mpo, oocysts Biology (General) QH301-705.5
spellingShingle	algeria, cryptosporidium, umbilicus rupestris, immunofluorescence, mpo, oocysts Biology (General) QH301-705.5 AFAF BENHOUDA DJAHIDA BENHOUDA MASSINISSA YAHIA In vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant Umbilicus rupestris
description	Abstract. Benhouda A, Benhouda D, Yahia M. 2019. In vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant Umbilicus rupestris. Biodiversitas 20: 3478-3483. Umbilicus rupestris (Crassulaceae) is a medicinal plant used in general traditional medicine to cure inflammation and irritation of the skin. The present research is aimed to evaluate the antiparasitic activity of the methanolic extract of the plant URMeOH of U. rupestris against the Cryptosporidium infection in immunocompetent and immunosuppressed rats experimentally infected. Twenty-one female rats were divided into two groups. Control group (group I) and experimental group (Group II). The group I was further divided into three equal groups (normal group infected and immunosuppressed infected group). The experimental group was divided into two immunosuppressed and four equal groups and two immunocompetent infected. Cryptosporidium oocysts were isolated from bovine species stools and used to infect rats. Experimental subgroups received URMeOH two as dose 100mg/kg b.w. and 200 mg/kg b.w. and continued until 15 days. Two weeks after the administration of URMeOH, feces of rats were examined for the detection of Cryptosporidium oocysts by Ziehl-Neelsen and immunofluorescence techniques, the animals were sacrificed; their small intestines were processed and examined for the detection of pathological lesions after histopathological study. In addition, the activity of myeloperoxidase (MPO) was measured in sections of the jejunum. Concerned the results, we observed a statistically significant (P<0.001) increase in the number of oocysts of Cryptosporidium in the stool for sub infected immunosuppressed groups and an increase of MPO activity compared to the corresponding subgroups immunocompetent subgroups. The URMeOH could remove Cryptosporidium oocysts from feces and intestinal sections subgroup infected immunocompetent rats receiving URMeOH. Moreover, the oocysts were significantly reduced in all other subgroups experimental infected compared to infected control subgroups. Intestinal sections in all subgroups received URMeOH revealed a more or less normal architecture. In addition, the reduction of MPO activity level was also detected in all experimental subgroups.
format	article
author	AFAF BENHOUDA DJAHIDA BENHOUDA MASSINISSA YAHIA
author_facet	AFAF BENHOUDA DJAHIDA BENHOUDA MASSINISSA YAHIA
author_sort	AFAF BENHOUDA
title	In vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant Umbilicus rupestris
title_short	In vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant Umbilicus rupestris
title_full	In vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant Umbilicus rupestris
title_fullStr	In vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant Umbilicus rupestris
title_full_unstemmed	In vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant Umbilicus rupestris
title_sort	in vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant umbilicus rupestris
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/2a647f7c4a804f65b45e24a9c72af572
work_keys_str_mv	AT afafbenhouda invivoevaluationofanticryptosporidiosisactivityofthemethanolicextractoftheplantumbilicusrupestris AT djahidabenhouda invivoevaluationofanticryptosporidiosisactivityofthemethanolicextractoftheplantumbilicusrupestris AT massinissayahia invivoevaluationofanticryptosporidiosisactivityofthemethanolicextractoftheplantumbilicusrupestris
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In vivo evaluation of anticryptosporidiosis activity of the methanolic extract of the plant Umbilicus rupestris

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