CircRNA_103765 acts as a proinflammatory factor via sponging miR-30 family in Crohn’s disease

Abstract Increasing evidence suggests that circular RNAs (circRNAs) play critical roles in various pathophysiological activities. However, the role of circRNAs in inflammatory bowel disease (IBD) remains unclear. Here we report the potential roles of hsa_circRNA_103765 in regulating cell apoptosis i...

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Autores principales:	Yulan Ye, Liping Zhang, Tong Hu, Juan Yin, Lijuan Xu, Zhi Pang, Weichang Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2a65a3c061ba4e9baf1d2c033b82f05f
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spelling	oai:doaj.org-article:2a65a3c061ba4e9baf1d2c033b82f05f2021-12-02T15:23:10ZCircRNA_103765 acts as a proinflammatory factor via sponging miR-30 family in Crohn’s disease10.1038/s41598-020-80663-w2045-2322https://doaj.org/article/2a65a3c061ba4e9baf1d2c033b82f05f2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80663-whttps://doaj.org/toc/2045-2322Abstract Increasing evidence suggests that circular RNAs (circRNAs) play critical roles in various pathophysiological activities. However, the role of circRNAs in inflammatory bowel disease (IBD) remains unclear. Here we report the potential roles of hsa_circRNA_103765 in regulating cell apoptosis induced by TNF-α in Crohn’s disease (CD). We identify that CircRNA_103765 expression was significantly upregulated in peripheral blood mononuclear cells (PBMCs) of patients with active IBD. A positive correlation with TNF-α significantly enhanced circRNA_103765 expression in CD, which was significantly reversed by anti-TNF-α mAb (infliximab) treatment. In vitro experiments showed that TNF-α could induce the expression of circRNA_103765, which was cell apoptosis dependent, while silencing of circRNA_103765 could protect human intestinal epithelial cells (IECs) from TNF-α-induced apoptosis. In addition, circRNA_103765 acted as a molecular sponge to adsorb the miR-30 family and impair the negative regulation of Delta-like ligand 4 (DLL4). Collectively, CircRNA_103765 is a novel important regulator of the pathogenesis of IBD via sponging miR-30 family-mediated DLL4 expression changes. Blockade of circRNA_103765 could serve as a novel approach for the treatment of IBD patients.Yulan YeLiping ZhangTong HuJuan YinLijuan XuZhi PangWeichang ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yulan Ye Liping Zhang Tong Hu Juan Yin Lijuan Xu Zhi Pang Weichang Chen CircRNA_103765 acts as a proinflammatory factor via sponging miR-30 family in Crohn’s disease
description	Abstract Increasing evidence suggests that circular RNAs (circRNAs) play critical roles in various pathophysiological activities. However, the role of circRNAs in inflammatory bowel disease (IBD) remains unclear. Here we report the potential roles of hsa_circRNA_103765 in regulating cell apoptosis induced by TNF-α in Crohn’s disease (CD). We identify that CircRNA_103765 expression was significantly upregulated in peripheral blood mononuclear cells (PBMCs) of patients with active IBD. A positive correlation with TNF-α significantly enhanced circRNA_103765 expression in CD, which was significantly reversed by anti-TNF-α mAb (infliximab) treatment. In vitro experiments showed that TNF-α could induce the expression of circRNA_103765, which was cell apoptosis dependent, while silencing of circRNA_103765 could protect human intestinal epithelial cells (IECs) from TNF-α-induced apoptosis. In addition, circRNA_103765 acted as a molecular sponge to adsorb the miR-30 family and impair the negative regulation of Delta-like ligand 4 (DLL4). Collectively, CircRNA_103765 is a novel important regulator of the pathogenesis of IBD via sponging miR-30 family-mediated DLL4 expression changes. Blockade of circRNA_103765 could serve as a novel approach for the treatment of IBD patients.
format	article
author	Yulan Ye Liping Zhang Tong Hu Juan Yin Lijuan Xu Zhi Pang Weichang Chen
author_facet	Yulan Ye Liping Zhang Tong Hu Juan Yin Lijuan Xu Zhi Pang Weichang Chen
author_sort	Yulan Ye
title	CircRNA_103765 acts as a proinflammatory factor via sponging miR-30 family in Crohn’s disease
title_short	CircRNA_103765 acts as a proinflammatory factor via sponging miR-30 family in Crohn’s disease
title_full	CircRNA_103765 acts as a proinflammatory factor via sponging miR-30 family in Crohn’s disease
title_fullStr	CircRNA_103765 acts as a proinflammatory factor via sponging miR-30 family in Crohn’s disease
title_full_unstemmed	CircRNA_103765 acts as a proinflammatory factor via sponging miR-30 family in Crohn’s disease
title_sort	circrna_103765 acts as a proinflammatory factor via sponging mir-30 family in crohn’s disease
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2a65a3c061ba4e9baf1d2c033b82f05f
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CircRNA_103765 acts as a proinflammatory factor via sponging miR-30 family in Crohn’s disease

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