Effects of cigarette smoking on the growth of Streptococcus mutans biofilms: An in vitro study.

The increased incidence of dental caries by cigarette smoking (CS) has been widely reported in epidemiological studies, but the relationship between CS and cariogenic biofilm growth has been rarely studied. This study aims to investigate the effects of CS exposure on the growth and virulence of Stre...

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Autor principal:	Ye Han
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2a66534c124a411992b79280fbfc23d3
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spelling	oai:doaj.org-article:2a66534c124a411992b79280fbfc23d32021-12-02T20:13:03ZEffects of cigarette smoking on the growth of Streptococcus mutans biofilms: An in vitro study.1932-620310.1371/journal.pone.0259895https://doaj.org/article/2a66534c124a411992b79280fbfc23d32021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0259895https://doaj.org/toc/1932-6203The increased incidence of dental caries by cigarette smoking (CS) has been widely reported in epidemiological studies, but the relationship between CS and cariogenic biofilm growth has been rarely studied. This study aims to investigate the effects of CS exposure on the growth and virulence of Streptococcus mutans biofilms (S. mutans). Briefly, S. mutans biofilms were formed on saliva-coated hydroxyapatite disks, which were exposed to CS 1, 3, and 6 times per day, respectively. In addition, S. mutans biofilms without CS exposure were considered as the control group. Acidogenicity, dry weight, colony-forming units (CFUs), water-soluble/insoluble extracellular polysaccharides (EPSs), and intracellular polysaccharides (IPSs) were analyzed and confocal laser scanning microscopy (CLSM) images of 74-h-old S. mutans biofilms were obtained. The lowest accumulation of biofilms and EPSs were detected in the 6 times/day CS exposure group compared with those of the control group and other CS exposure groups in 74-h-old S. mutans biofilms. CLSM also revealed the lowest bacterial count (live and dead cells) and EPSs biovolume in the six times/day CS exposure group in 74-h-old S. mutans biofilms. CS exposure inhibited the growth of S. mutans biofilm in vitro study, the anti-cariogenic biofilm formation was enhanced with a dose (frequency)-dependent at which frequency has more influence in the present findings.Ye HanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11, p e0259895 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ye Han Effects of cigarette smoking on the growth of Streptococcus mutans biofilms: An in vitro study.
description	The increased incidence of dental caries by cigarette smoking (CS) has been widely reported in epidemiological studies, but the relationship between CS and cariogenic biofilm growth has been rarely studied. This study aims to investigate the effects of CS exposure on the growth and virulence of Streptococcus mutans biofilms (S. mutans). Briefly, S. mutans biofilms were formed on saliva-coated hydroxyapatite disks, which were exposed to CS 1, 3, and 6 times per day, respectively. In addition, S. mutans biofilms without CS exposure were considered as the control group. Acidogenicity, dry weight, colony-forming units (CFUs), water-soluble/insoluble extracellular polysaccharides (EPSs), and intracellular polysaccharides (IPSs) were analyzed and confocal laser scanning microscopy (CLSM) images of 74-h-old S. mutans biofilms were obtained. The lowest accumulation of biofilms and EPSs were detected in the 6 times/day CS exposure group compared with those of the control group and other CS exposure groups in 74-h-old S. mutans biofilms. CLSM also revealed the lowest bacterial count (live and dead cells) and EPSs biovolume in the six times/day CS exposure group in 74-h-old S. mutans biofilms. CS exposure inhibited the growth of S. mutans biofilm in vitro study, the anti-cariogenic biofilm formation was enhanced with a dose (frequency)-dependent at which frequency has more influence in the present findings.
format	article
author	Ye Han
author_facet	Ye Han
author_sort	Ye Han
title	Effects of cigarette smoking on the growth of Streptococcus mutans biofilms: An in vitro study.
title_short	Effects of cigarette smoking on the growth of Streptococcus mutans biofilms: An in vitro study.
title_full	Effects of cigarette smoking on the growth of Streptococcus mutans biofilms: An in vitro study.
title_fullStr	Effects of cigarette smoking on the growth of Streptococcus mutans biofilms: An in vitro study.
title_full_unstemmed	Effects of cigarette smoking on the growth of Streptococcus mutans biofilms: An in vitro study.
title_sort	effects of cigarette smoking on the growth of streptococcus mutans biofilms: an in vitro study.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/2a66534c124a411992b79280fbfc23d3
work_keys_str_mv	AT yehan effectsofcigarettesmokingonthegrowthofstreptococcusmutansbiofilmsaninvitrostudy
_version_	1718374813435691008

Effects of cigarette smoking on the growth of Streptococcus mutans biofilms: An in vitro study.

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