Nonconformities in Veterinary Cytopathological Examinations: A Retrospective Study of Unsuitable Samples for Analysis

The aim of this study was to evaluate the main nonconformities that result in cytopathological samples inappropriate for diagnosis in veterinary medicine. All cytopathological samples, obtained from different canine and feline tissues/lesions, included in the study were received and classified as in...

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Autores principales:	Silva Melina Barreto Gomes da, Pascoal Ingrid Cavalcanti, Silva Órion Pedro da, Alves Amanda de Deus Ferreira, Gonçalves Saulo Romero Félix, Barretto Mariana Lumack do Monte, Pinheiro Junior José Wilton, Pereira Márcia de Figueiredo, Oliveira Andrea Alice da Fonseca
Formato:	article
Lenguaje:	EN
Publicado:	Sciendo 2019
Materias:	canine cytopathology feline quality assurance sample processing Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/2a68e43c96614ae8b385574a31cc0ddf
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spelling	oai:doaj.org-article:2a68e43c96614ae8b385574a31cc0ddf2021-11-17T21:27:53ZNonconformities in Veterinary Cytopathological Examinations: A Retrospective Study of Unsuitable Samples for Analysis1820-744810.2478/acve-2019-0021https://doaj.org/article/2a68e43c96614ae8b385574a31cc0ddf2019-09-01T00:00:00Zhttps://doi.org/10.2478/acve-2019-0021https://doaj.org/toc/1820-7448The aim of this study was to evaluate the main nonconformities that result in cytopathological samples inappropriate for diagnosis in veterinary medicine. All cytopathological samples, obtained from different canine and feline tissues/lesions, included in the study were received and classified as inconclusive by a single public laboratory of veterinary pathology, located in Pernambuco State, Brazil, between 2012 and 2016. Nonconformities regarding the smear quality, cellularity, presence or absence of hemorrhage, cellular overlapping, desiccation, and presence or absence of necrotic debris and/or artifacts were evaluated. Data were tabulated using Microsoft Excel 2007; absolute and relative frequencies were calculated using EPIINFO 3.5.2. From the 3268 cases received between 2012 and 2016, 190 cases were selected and comprised 514 inconclusive slides. The most frequent nonconformities detected were insufficient/absence of cellularity in 100% (514/514), inadequate submacroscopic presentation in 87% (446/514), and hemorrhage in 69% (356/514) of samples. Other features identified were cellular overlapping in 34% (175/514), inadequate staining in 31% (175/514), artifacts in 30% (154/514), desiccation in 28% (145/514), and necrotic debris in 26% (133/514) of samples. The implementation of laboratory standard operational procedures aimed at maintaining quality is essential. It is necessary to initially identify the main errors occurring in the processing stages as a way to guide and design strategies to avoid them.Silva Melina Barreto Gomes daPascoal Ingrid CavalcantiSilva Órion Pedro daAlves Amanda de Deus FerreiraGonçalves Saulo Romero FélixBarretto Mariana Lumack do MontePinheiro Junior José WiltonPereira Márcia de FigueiredoOliveira Andrea Alice da FonsecaSciendoarticlecaninecytopathologyfelinequality assurancesample processingVeterinary medicineSF600-1100ENActa Veterinaria, Vol 69, Iss 3, Pp 251-261 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	canine cytopathology feline quality assurance sample processing Veterinary medicine SF600-1100
spellingShingle	canine cytopathology feline quality assurance sample processing Veterinary medicine SF600-1100 Silva Melina Barreto Gomes da Pascoal Ingrid Cavalcanti Silva Órion Pedro da Alves Amanda de Deus Ferreira Gonçalves Saulo Romero Félix Barretto Mariana Lumack do Monte Pinheiro Junior José Wilton Pereira Márcia de Figueiredo Oliveira Andrea Alice da Fonseca Nonconformities in Veterinary Cytopathological Examinations: A Retrospective Study of Unsuitable Samples for Analysis
description	The aim of this study was to evaluate the main nonconformities that result in cytopathological samples inappropriate for diagnosis in veterinary medicine. All cytopathological samples, obtained from different canine and feline tissues/lesions, included in the study were received and classified as inconclusive by a single public laboratory of veterinary pathology, located in Pernambuco State, Brazil, between 2012 and 2016. Nonconformities regarding the smear quality, cellularity, presence or absence of hemorrhage, cellular overlapping, desiccation, and presence or absence of necrotic debris and/or artifacts were evaluated. Data were tabulated using Microsoft Excel 2007; absolute and relative frequencies were calculated using EPIINFO 3.5.2. From the 3268 cases received between 2012 and 2016, 190 cases were selected and comprised 514 inconclusive slides. The most frequent nonconformities detected were insufficient/absence of cellularity in 100% (514/514), inadequate submacroscopic presentation in 87% (446/514), and hemorrhage in 69% (356/514) of samples. Other features identified were cellular overlapping in 34% (175/514), inadequate staining in 31% (175/514), artifacts in 30% (154/514), desiccation in 28% (145/514), and necrotic debris in 26% (133/514) of samples. The implementation of laboratory standard operational procedures aimed at maintaining quality is essential. It is necessary to initially identify the main errors occurring in the processing stages as a way to guide and design strategies to avoid them.
format	article
author	Silva Melina Barreto Gomes da Pascoal Ingrid Cavalcanti Silva Órion Pedro da Alves Amanda de Deus Ferreira Gonçalves Saulo Romero Félix Barretto Mariana Lumack do Monte Pinheiro Junior José Wilton Pereira Márcia de Figueiredo Oliveira Andrea Alice da Fonseca
author_facet	Silva Melina Barreto Gomes da Pascoal Ingrid Cavalcanti Silva Órion Pedro da Alves Amanda de Deus Ferreira Gonçalves Saulo Romero Félix Barretto Mariana Lumack do Monte Pinheiro Junior José Wilton Pereira Márcia de Figueiredo Oliveira Andrea Alice da Fonseca
author_sort	Silva Melina Barreto Gomes da
title	Nonconformities in Veterinary Cytopathological Examinations: A Retrospective Study of Unsuitable Samples for Analysis
title_short	Nonconformities in Veterinary Cytopathological Examinations: A Retrospective Study of Unsuitable Samples for Analysis
title_full	Nonconformities in Veterinary Cytopathological Examinations: A Retrospective Study of Unsuitable Samples for Analysis
title_fullStr	Nonconformities in Veterinary Cytopathological Examinations: A Retrospective Study of Unsuitable Samples for Analysis
title_full_unstemmed	Nonconformities in Veterinary Cytopathological Examinations: A Retrospective Study of Unsuitable Samples for Analysis
title_sort	nonconformities in veterinary cytopathological examinations: a retrospective study of unsuitable samples for analysis
publisher	Sciendo
publishDate	2019
url	https://doaj.org/article/2a68e43c96614ae8b385574a31cc0ddf
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Nonconformities in Veterinary Cytopathological Examinations: A Retrospective Study of Unsuitable Samples for Analysis

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