A method for the identification of COVID-19 biomarkers in human breath using Proton Transfer Reaction Time-of-Flight Mass Spectrometry

Background: COVID-19 has caused a worldwide pandemic, making the early detection of the virus crucial. We present an approach for the determination of COVID-19 infection based on breath analysis. Methods: A high sensitivity mass spectrometer was combined with artificial intelligence and used to deve...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Aikaterini Liangou, MSc, Antonios Tasoglou, PhD, Heinz J. Huber, PhD, Christopher Wistrom, DO, Kevin Brody, MD, Prahlad G Menon, PhD, Thomas Bebekoski, MD, Kevin Menschel, DO, Marlise Davidson-Fiedler, MD, Karl DeMarco, MD, Harshad Salphale, Jonathan Wistrom, Skyler Wistrom, Richard J. Lee, PhD
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2a708de2fc0344f5aae84231a0e791f9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2a708de2fc0344f5aae84231a0e791f9
record_format	dspace
spelling	oai:doaj.org-article:2a708de2fc0344f5aae84231a0e791f92021-11-22T04:29:03ZA method for the identification of COVID-19 biomarkers in human breath using Proton Transfer Reaction Time-of-Flight Mass Spectrometry2589-537010.1016/j.eclinm.2021.101207https://doaj.org/article/2a708de2fc0344f5aae84231a0e791f92021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589537021004880https://doaj.org/toc/2589-5370Background: COVID-19 has caused a worldwide pandemic, making the early detection of the virus crucial. We present an approach for the determination of COVID-19 infection based on breath analysis. Methods: A high sensitivity mass spectrometer was combined with artificial intelligence and used to develop a method for the identification of COVID-19 in human breath within seconds. A set of 1137 positive and negative subjects from different age groups, collected in two periods from two hospitals in the USA, from 26 August, 2020 until 15 September, 2020 and from 11 September, 2020 until 11 November, 2020, was used for the method development. The subjects exhaled in a Tedlar bag, and the exhaled breath samples were subsequently analyzed using a Proton Transfer Reaction Time-of-Flight Mass Spectrometer (PTR-ToF-MS). The produced mass spectra were introduced to a series of machine learning models. 70% of the data was used for these sub-models’ training and 30% was used for testing. Findings: A set of 340 samples, 95 positives and 245 negatives, was used for the testing. The combined models successfully predicted 77 out of the 95 samples as positives and 199 out of the 245 samples as negatives. The overall accuracy of the model was 81.2%. Since over 50% of the total positive samples belonged to the age group of over 55 years old, the performance of the model in this category was also separately evaluated on 339 subjects (170 negative and 169 positive). The model correctly identified 166 out of the 170 negatives and 164 out of the 169 positives. The model accuracy in this case was 97.3%. Interpretation: The results showed that this method for the identification of COVID-19 infection is a promising tool, which can give fast and accurate results.Aikaterini Liangou, MScAntonios Tasoglou, PhDHeinz J. Huber, PhDChristopher Wistrom, DOKevin Brody, MDPrahlad G Menon, PhDThomas Bebekoski, MDKevin Menschel, DOMarlise Davidson-Fiedler, MDKarl DeMarco, MDHarshad SalphaleJonathan WistromSkyler WistromRichard J. Lee, PhDElsevierarticleMedicine (General)R5-920ENEClinicalMedicine, Vol 42, Iss , Pp 101207- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Aikaterini Liangou, MSc Antonios Tasoglou, PhD Heinz J. Huber, PhD Christopher Wistrom, DO Kevin Brody, MD Prahlad G Menon, PhD Thomas Bebekoski, MD Kevin Menschel, DO Marlise Davidson-Fiedler, MD Karl DeMarco, MD Harshad Salphale Jonathan Wistrom Skyler Wistrom Richard J. Lee, PhD A method for the identification of COVID-19 biomarkers in human breath using Proton Transfer Reaction Time-of-Flight Mass Spectrometry
description	Background: COVID-19 has caused a worldwide pandemic, making the early detection of the virus crucial. We present an approach for the determination of COVID-19 infection based on breath analysis. Methods: A high sensitivity mass spectrometer was combined with artificial intelligence and used to develop a method for the identification of COVID-19 in human breath within seconds. A set of 1137 positive and negative subjects from different age groups, collected in two periods from two hospitals in the USA, from 26 August, 2020 until 15 September, 2020 and from 11 September, 2020 until 11 November, 2020, was used for the method development. The subjects exhaled in a Tedlar bag, and the exhaled breath samples were subsequently analyzed using a Proton Transfer Reaction Time-of-Flight Mass Spectrometer (PTR-ToF-MS). The produced mass spectra were introduced to a series of machine learning models. 70% of the data was used for these sub-models’ training and 30% was used for testing. Findings: A set of 340 samples, 95 positives and 245 negatives, was used for the testing. The combined models successfully predicted 77 out of the 95 samples as positives and 199 out of the 245 samples as negatives. The overall accuracy of the model was 81.2%. Since over 50% of the total positive samples belonged to the age group of over 55 years old, the performance of the model in this category was also separately evaluated on 339 subjects (170 negative and 169 positive). The model correctly identified 166 out of the 170 negatives and 164 out of the 169 positives. The model accuracy in this case was 97.3%. Interpretation: The results showed that this method for the identification of COVID-19 infection is a promising tool, which can give fast and accurate results.
format	article
author	Aikaterini Liangou, MSc Antonios Tasoglou, PhD Heinz J. Huber, PhD Christopher Wistrom, DO Kevin Brody, MD Prahlad G Menon, PhD Thomas Bebekoski, MD Kevin Menschel, DO Marlise Davidson-Fiedler, MD Karl DeMarco, MD Harshad Salphale Jonathan Wistrom Skyler Wistrom Richard J. Lee, PhD
author_facet	Aikaterini Liangou, MSc Antonios Tasoglou, PhD Heinz J. Huber, PhD Christopher Wistrom, DO Kevin Brody, MD Prahlad G Menon, PhD Thomas Bebekoski, MD Kevin Menschel, DO Marlise Davidson-Fiedler, MD Karl DeMarco, MD Harshad Salphale Jonathan Wistrom Skyler Wistrom Richard J. Lee, PhD
author_sort	Aikaterini Liangou, MSc
title	A method for the identification of COVID-19 biomarkers in human breath using Proton Transfer Reaction Time-of-Flight Mass Spectrometry
title_short	A method for the identification of COVID-19 biomarkers in human breath using Proton Transfer Reaction Time-of-Flight Mass Spectrometry
title_full	A method for the identification of COVID-19 biomarkers in human breath using Proton Transfer Reaction Time-of-Flight Mass Spectrometry
title_fullStr	A method for the identification of COVID-19 biomarkers in human breath using Proton Transfer Reaction Time-of-Flight Mass Spectrometry
title_full_unstemmed	A method for the identification of COVID-19 biomarkers in human breath using Proton Transfer Reaction Time-of-Flight Mass Spectrometry
title_sort	method for the identification of covid-19 biomarkers in human breath using proton transfer reaction time-of-flight mass spectrometry
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2a708de2fc0344f5aae84231a0e791f9
work_keys_str_mv	AT aikateriniliangoumsc amethodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT antoniostasoglouphd amethodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT heinzjhuberphd amethodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT christopherwistromdo amethodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT kevinbrodymd amethodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT prahladgmenonphd amethodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT thomasbebekoskimd amethodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT kevinmenscheldo amethodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT marlisedavidsonfiedlermd amethodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT karldemarcomd amethodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT harshadsalphale amethodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT jonathanwistrom amethodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT skylerwistrom amethodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT richardjleephd amethodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT aikateriniliangoumsc methodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT antoniostasoglouphd methodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT heinzjhuberphd methodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT christopherwistromdo methodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT kevinbrodymd methodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT prahladgmenonphd methodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT thomasbebekoskimd methodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT kevinmenscheldo methodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT marlisedavidsonfiedlermd methodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT karldemarcomd methodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT harshadsalphale methodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT jonathanwistrom methodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT skylerwistrom methodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry AT richardjleephd methodfortheidentificationofcovid19biomarkersinhumanbreathusingprotontransferreactiontimeofflightmassspectrometry
_version_	1718418151808434176

A method for the identification of COVID-19 biomarkers in human breath using Proton Transfer Reaction Time-of-Flight Mass Spectrometry

Ejemplares similares