Potential of CO2-laser processing of quartz for fast prototyping of microfluidic reactors and templates for 3D cell assembly over large scale

Carbon dioxide (CO2)-laser processing of glasses is a versatile maskless writing technique to engrave micro-structures with flexible control on shape and size.In this study, we present the fabrication of hundreds of microns quartz micro-channels and micro-holes by pulsed CO2-laser ablation with a fo...

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Autores principales:	Elisabetta Perrone, Maura Cesaria, Alessandra Zizzari, Monica Bianco, Francesco Ferrara, Lillo Raia, Vita Guarino, Massimo Cuscunà, Marco Mazzeo, Giuseppe Gigli, Lorenzo Moroni, Valentina Arima
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	CO2-laser ablation Microfluidics Micro-reactors 3D cell assembly Lab-on-a-Chip Organ-on-a-Chip Medicine (General) R5-920 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2a793ef3f5fa49b2b1de6886d6261c58
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spelling	oai:doaj.org-article:2a793ef3f5fa49b2b1de6886d6261c582021-11-26T04:38:44ZPotential of CO2-laser processing of quartz for fast prototyping of microfluidic reactors and templates for 3D cell assembly over large scale2590-006410.1016/j.mtbio.2021.100163https://doaj.org/article/2a793ef3f5fa49b2b1de6886d6261c582021-09-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2590006421000715https://doaj.org/toc/2590-0064Carbon dioxide (CO2)-laser processing of glasses is a versatile maskless writing technique to engrave micro-structures with flexible control on shape and size.In this study, we present the fabrication of hundreds of microns quartz micro-channels and micro-holes by pulsed CO2-laser ablation with a focus on the great potential of the technique in microfluidics and biomedical applications. After discussing the impact of the laser processing parameters on the design process, we illustrate specific applications. First, we demonstrate the use of a serpentine microfluidic reactor prepared by combining CO2-laser ablation and post-ablation wet etching to remove surface features stemming from laser-texturing that are undesirable for channel sealing. Then, cyclic olefin copolymer micro-pillars are fabricated using laser-processed micro-holes as molds with high detail replication. The hundreds of microns conical and square pyramidal shaped pillars are used as templates to drive 3D cell assembly. Human Umbilical Vein Endothelial Cells are found to assemble in a compact and wrapping way around the micro-pillars forming a tight junction network. These applications are interesting for both Lab-on-a-Chip and Organ-on-a-Chip devices.Elisabetta PerroneMaura CesariaAlessandra ZizzariMonica BiancoFrancesco FerraraLillo RaiaVita GuarinoMassimo CuscunàMarco MazzeoGiuseppe GigliLorenzo MoroniValentina ArimaElsevierarticleCO2-laser ablationMicrofluidicsMicro-reactors3D cell assemblyLab-on-a-ChipOrgan-on-a-ChipMedicine (General)R5-920Biology (General)QH301-705.5ENMaterials Today Bio, Vol 12, Iss , Pp 100163- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	CO2-laser ablation Microfluidics Micro-reactors 3D cell assembly Lab-on-a-Chip Organ-on-a-Chip Medicine (General) R5-920 Biology (General) QH301-705.5
spellingShingle	CO2-laser ablation Microfluidics Micro-reactors 3D cell assembly Lab-on-a-Chip Organ-on-a-Chip Medicine (General) R5-920 Biology (General) QH301-705.5 Elisabetta Perrone Maura Cesaria Alessandra Zizzari Monica Bianco Francesco Ferrara Lillo Raia Vita Guarino Massimo Cuscunà Marco Mazzeo Giuseppe Gigli Lorenzo Moroni Valentina Arima Potential of CO2-laser processing of quartz for fast prototyping of microfluidic reactors and templates for 3D cell assembly over large scale
description	Carbon dioxide (CO2)-laser processing of glasses is a versatile maskless writing technique to engrave micro-structures with flexible control on shape and size.In this study, we present the fabrication of hundreds of microns quartz micro-channels and micro-holes by pulsed CO2-laser ablation with a focus on the great potential of the technique in microfluidics and biomedical applications. After discussing the impact of the laser processing parameters on the design process, we illustrate specific applications. First, we demonstrate the use of a serpentine microfluidic reactor prepared by combining CO2-laser ablation and post-ablation wet etching to remove surface features stemming from laser-texturing that are undesirable for channel sealing. Then, cyclic olefin copolymer micro-pillars are fabricated using laser-processed micro-holes as molds with high detail replication. The hundreds of microns conical and square pyramidal shaped pillars are used as templates to drive 3D cell assembly. Human Umbilical Vein Endothelial Cells are found to assemble in a compact and wrapping way around the micro-pillars forming a tight junction network. These applications are interesting for both Lab-on-a-Chip and Organ-on-a-Chip devices.
format	article
author	Elisabetta Perrone Maura Cesaria Alessandra Zizzari Monica Bianco Francesco Ferrara Lillo Raia Vita Guarino Massimo Cuscunà Marco Mazzeo Giuseppe Gigli Lorenzo Moroni Valentina Arima
author_facet	Elisabetta Perrone Maura Cesaria Alessandra Zizzari Monica Bianco Francesco Ferrara Lillo Raia Vita Guarino Massimo Cuscunà Marco Mazzeo Giuseppe Gigli Lorenzo Moroni Valentina Arima
author_sort	Elisabetta Perrone
title	Potential of CO2-laser processing of quartz for fast prototyping of microfluidic reactors and templates for 3D cell assembly over large scale
title_short	Potential of CO2-laser processing of quartz for fast prototyping of microfluidic reactors and templates for 3D cell assembly over large scale
title_full	Potential of CO2-laser processing of quartz for fast prototyping of microfluidic reactors and templates for 3D cell assembly over large scale
title_fullStr	Potential of CO2-laser processing of quartz for fast prototyping of microfluidic reactors and templates for 3D cell assembly over large scale
title_full_unstemmed	Potential of CO2-laser processing of quartz for fast prototyping of microfluidic reactors and templates for 3D cell assembly over large scale
title_sort	potential of co2-laser processing of quartz for fast prototyping of microfluidic reactors and templates for 3d cell assembly over large scale
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/2a793ef3f5fa49b2b1de6886d6261c58
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Potential of CO2-laser processing of quartz for fast prototyping of microfluidic reactors and templates for 3D cell assembly over large scale

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