Epigenetic and phenotypic profile of fibroblasts derived from induced pluripotent stem cells.

Human induced pluripotent stem (hiPS) cells offer a novel source of patient-specific cells for regenerative medicine. However, the biological potential of iPS-derived cells and their similarities to cells differentiated from human embryonic stem (hES) cells remain unclear. We derived fibroblast-like...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kyle J Hewitt, Yulia Shamis, Ryan B Hayman, Mariam Margvelashvili, Shumin Dong, Mark W Carlson, Jonathan A Garlick
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2a7cb5367ce34251aacea31d4b8a9af2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2a7cb5367ce34251aacea31d4b8a9af2
record_format	dspace
spelling	oai:doaj.org-article:2a7cb5367ce34251aacea31d4b8a9af22021-11-18T06:58:08ZEpigenetic and phenotypic profile of fibroblasts derived from induced pluripotent stem cells.1932-620310.1371/journal.pone.0017128https://doaj.org/article/2a7cb5367ce34251aacea31d4b8a9af22011-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21386890/?tool=EBIhttps://doaj.org/toc/1932-6203Human induced pluripotent stem (hiPS) cells offer a novel source of patient-specific cells for regenerative medicine. However, the biological potential of iPS-derived cells and their similarities to cells differentiated from human embryonic stem (hES) cells remain unclear. We derived fibroblast-like cells from two hiPS cell lines and show that their phenotypic properties and patterns of DNA methylation were similar to that of mature fibroblasts and to fibroblasts derived from hES cells. iPS-derived fibroblasts (iPDK) and their hES-derived counterparts (EDK) showed similar cell morphology throughout differentiation, and patterns of gene expression and cell surface markers were characteristic of mature fibroblasts. Array-based methylation analysis was performed for EDK, iPDK and their parental hES and iPS cell lines, and hierarchical clustering revealed that EDK and iPDK had closely-related methylation profiles. DNA methylation analysis of promoter regions associated with extracellular matrix (ECM)-production (COL1A1) by iPS- and hESC-derived fibroblasts and fibroblast lineage commitment (PDGFRβ), revealed promoter demethylation linked to their expression, and patterns of transcription and methylation of genes related to the functional properties of mature stromal cells were seen in both hiPS- and hES-derived fibroblasts. iPDK cells also showed functional properties analogous to those of hES-derived and mature fibroblasts, as seen by their capacity to direct the morphogenesis of engineered human skin equivalents. Characterization of the functional behavior of ES- and iPS-derived fibroblasts in engineered 3D tissues demonstrates the utility of this tissue platform to predict the capacity of iPS-derived cells before their therapeutic application.Kyle J HewittYulia ShamisRyan B HaymanMariam MargvelashviliShumin DongMark W CarlsonJonathan A GarlickPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 2, p e17128 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kyle J Hewitt Yulia Shamis Ryan B Hayman Mariam Margvelashvili Shumin Dong Mark W Carlson Jonathan A Garlick Epigenetic and phenotypic profile of fibroblasts derived from induced pluripotent stem cells.
description	Human induced pluripotent stem (hiPS) cells offer a novel source of patient-specific cells for regenerative medicine. However, the biological potential of iPS-derived cells and their similarities to cells differentiated from human embryonic stem (hES) cells remain unclear. We derived fibroblast-like cells from two hiPS cell lines and show that their phenotypic properties and patterns of DNA methylation were similar to that of mature fibroblasts and to fibroblasts derived from hES cells. iPS-derived fibroblasts (iPDK) and their hES-derived counterparts (EDK) showed similar cell morphology throughout differentiation, and patterns of gene expression and cell surface markers were characteristic of mature fibroblasts. Array-based methylation analysis was performed for EDK, iPDK and their parental hES and iPS cell lines, and hierarchical clustering revealed that EDK and iPDK had closely-related methylation profiles. DNA methylation analysis of promoter regions associated with extracellular matrix (ECM)-production (COL1A1) by iPS- and hESC-derived fibroblasts and fibroblast lineage commitment (PDGFRβ), revealed promoter demethylation linked to their expression, and patterns of transcription and methylation of genes related to the functional properties of mature stromal cells were seen in both hiPS- and hES-derived fibroblasts. iPDK cells also showed functional properties analogous to those of hES-derived and mature fibroblasts, as seen by their capacity to direct the morphogenesis of engineered human skin equivalents. Characterization of the functional behavior of ES- and iPS-derived fibroblasts in engineered 3D tissues demonstrates the utility of this tissue platform to predict the capacity of iPS-derived cells before their therapeutic application.
format	article
author	Kyle J Hewitt Yulia Shamis Ryan B Hayman Mariam Margvelashvili Shumin Dong Mark W Carlson Jonathan A Garlick
author_facet	Kyle J Hewitt Yulia Shamis Ryan B Hayman Mariam Margvelashvili Shumin Dong Mark W Carlson Jonathan A Garlick
author_sort	Kyle J Hewitt
title	Epigenetic and phenotypic profile of fibroblasts derived from induced pluripotent stem cells.
title_short	Epigenetic and phenotypic profile of fibroblasts derived from induced pluripotent stem cells.
title_full	Epigenetic and phenotypic profile of fibroblasts derived from induced pluripotent stem cells.
title_fullStr	Epigenetic and phenotypic profile of fibroblasts derived from induced pluripotent stem cells.
title_full_unstemmed	Epigenetic and phenotypic profile of fibroblasts derived from induced pluripotent stem cells.
title_sort	epigenetic and phenotypic profile of fibroblasts derived from induced pluripotent stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/2a7cb5367ce34251aacea31d4b8a9af2
work_keys_str_mv	AT kylejhewitt epigeneticandphenotypicprofileoffibroblastsderivedfrominducedpluripotentstemcells AT yuliashamis epigeneticandphenotypicprofileoffibroblastsderivedfrominducedpluripotentstemcells AT ryanbhayman epigeneticandphenotypicprofileoffibroblastsderivedfrominducedpluripotentstemcells AT mariammargvelashvili epigeneticandphenotypicprofileoffibroblastsderivedfrominducedpluripotentstemcells AT shumindong epigeneticandphenotypicprofileoffibroblastsderivedfrominducedpluripotentstemcells AT markwcarlson epigeneticandphenotypicprofileoffibroblastsderivedfrominducedpluripotentstemcells AT jonathanagarlick epigeneticandphenotypicprofileoffibroblastsderivedfrominducedpluripotentstemcells
_version_	1718424089581846528

Epigenetic and phenotypic profile of fibroblasts derived from induced pluripotent stem cells.

Ejemplares similares