Characterization of pro-inflammatory flagellin proteins produced by Lactobacillus ruminis and related motile Lactobacilli.

Lactobacillus ruminis is one of at least twelve motile but poorly characterized species found in the genus Lactobacillus. Of these, only L. ruminis has been isolated from mammals, and this species may be considered as an autochthonous member of the gastrointestinal microbiota of humans, pigs and cow...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	B Anne Neville, Brian M Forde, Marcus J Claesson, Trevor Darby, Avril Coghlan, Kenneth Nally, R Paul Ross, Paul W O'Toole
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2a7f856d9cde40a08abe1a4778464ab7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2a7f856d9cde40a08abe1a4778464ab7
record_format	dspace
spelling	oai:doaj.org-article:2a7f856d9cde40a08abe1a4778464ab72021-11-18T07:12:55ZCharacterization of pro-inflammatory flagellin proteins produced by Lactobacillus ruminis and related motile Lactobacilli.1932-620310.1371/journal.pone.0040592https://doaj.org/article/2a7f856d9cde40a08abe1a4778464ab72012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22808200/?tool=EBIhttps://doaj.org/toc/1932-6203Lactobacillus ruminis is one of at least twelve motile but poorly characterized species found in the genus Lactobacillus. Of these, only L. ruminis has been isolated from mammals, and this species may be considered as an autochthonous member of the gastrointestinal microbiota of humans, pigs and cows. Nine L. ruminis strains were investigated here to elucidate the biochemistry and genetics of Lactobacillus motility. Six strains isolated from humans were non-motile while three bovine isolates were motile. A complete set of flagellum biogenesis genes was annotated in the sequenced genomes of two strains, ATCC25644 (human isolate) and ATCC27782 (bovine isolate), but only the latter strain produced flagella. Comparison of the L. ruminis and L. mali DSM20444(T) motility loci showed that their genetic content and gene-order were broadly similar, although the L. mali motility locus was interrupted by an 11.8 Kb region encoding rhamnose utilization genes that is absent from the L. ruminis motility locus. Phylogenetic analysis of 39 motile bacteria indicated that Lactobacillus motility genes were most closely related to those of motile carnobacteria and enterococci. Transcriptome analysis revealed that motility genes were transcribed at a significantly higher level in motile L. ruminis ATCC27782 than in non-motile ATCC25644. Flagellin proteins were isolated from L. ruminis ATCC27782 and from three other Lactobacillus species, while recombinant flagellin of aflagellate L. ruminis ATCC25644 was expressed and purified from E. coli. These native and recombinant Lactobacillus flagellins, and also flagellate L. ruminis cells, triggered interleukin-8 production in cultured human intestinal epithelial cells in a manner suppressed by short interfering RNA directed against Toll-Like Receptor 5. This study provides genetic, transcriptomic, phylogenetic and immunological insights into the trait of flagellum-mediated motility in the lactobacilli.B Anne NevilleBrian M FordeMarcus J ClaessonTrevor DarbyAvril CoghlanKenneth NallyR Paul RossPaul W O'ToolePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 7, p e40592 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q B Anne Neville Brian M Forde Marcus J Claesson Trevor Darby Avril Coghlan Kenneth Nally R Paul Ross Paul W O'Toole Characterization of pro-inflammatory flagellin proteins produced by Lactobacillus ruminis and related motile Lactobacilli.
description	Lactobacillus ruminis is one of at least twelve motile but poorly characterized species found in the genus Lactobacillus. Of these, only L. ruminis has been isolated from mammals, and this species may be considered as an autochthonous member of the gastrointestinal microbiota of humans, pigs and cows. Nine L. ruminis strains were investigated here to elucidate the biochemistry and genetics of Lactobacillus motility. Six strains isolated from humans were non-motile while three bovine isolates were motile. A complete set of flagellum biogenesis genes was annotated in the sequenced genomes of two strains, ATCC25644 (human isolate) and ATCC27782 (bovine isolate), but only the latter strain produced flagella. Comparison of the L. ruminis and L. mali DSM20444(T) motility loci showed that their genetic content and gene-order were broadly similar, although the L. mali motility locus was interrupted by an 11.8 Kb region encoding rhamnose utilization genes that is absent from the L. ruminis motility locus. Phylogenetic analysis of 39 motile bacteria indicated that Lactobacillus motility genes were most closely related to those of motile carnobacteria and enterococci. Transcriptome analysis revealed that motility genes were transcribed at a significantly higher level in motile L. ruminis ATCC27782 than in non-motile ATCC25644. Flagellin proteins were isolated from L. ruminis ATCC27782 and from three other Lactobacillus species, while recombinant flagellin of aflagellate L. ruminis ATCC25644 was expressed and purified from E. coli. These native and recombinant Lactobacillus flagellins, and also flagellate L. ruminis cells, triggered interleukin-8 production in cultured human intestinal epithelial cells in a manner suppressed by short interfering RNA directed against Toll-Like Receptor 5. This study provides genetic, transcriptomic, phylogenetic and immunological insights into the trait of flagellum-mediated motility in the lactobacilli.
format	article
author	B Anne Neville Brian M Forde Marcus J Claesson Trevor Darby Avril Coghlan Kenneth Nally R Paul Ross Paul W O'Toole
author_facet	B Anne Neville Brian M Forde Marcus J Claesson Trevor Darby Avril Coghlan Kenneth Nally R Paul Ross Paul W O'Toole
author_sort	B Anne Neville
title	Characterization of pro-inflammatory flagellin proteins produced by Lactobacillus ruminis and related motile Lactobacilli.
title_short	Characterization of pro-inflammatory flagellin proteins produced by Lactobacillus ruminis and related motile Lactobacilli.
title_full	Characterization of pro-inflammatory flagellin proteins produced by Lactobacillus ruminis and related motile Lactobacilli.
title_fullStr	Characterization of pro-inflammatory flagellin proteins produced by Lactobacillus ruminis and related motile Lactobacilli.
title_full_unstemmed	Characterization of pro-inflammatory flagellin proteins produced by Lactobacillus ruminis and related motile Lactobacilli.
title_sort	characterization of pro-inflammatory flagellin proteins produced by lactobacillus ruminis and related motile lactobacilli.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2a7f856d9cde40a08abe1a4778464ab7
work_keys_str_mv	AT banneneville characterizationofproinflammatoryflagellinproteinsproducedbylactobacillusruminisandrelatedmotilelactobacilli AT brianmforde characterizationofproinflammatoryflagellinproteinsproducedbylactobacillusruminisandrelatedmotilelactobacilli AT marcusjclaesson characterizationofproinflammatoryflagellinproteinsproducedbylactobacillusruminisandrelatedmotilelactobacilli AT trevordarby characterizationofproinflammatoryflagellinproteinsproducedbylactobacillusruminisandrelatedmotilelactobacilli AT avrilcoghlan characterizationofproinflammatoryflagellinproteinsproducedbylactobacillusruminisandrelatedmotilelactobacilli AT kennethnally characterizationofproinflammatoryflagellinproteinsproducedbylactobacillusruminisandrelatedmotilelactobacilli AT rpaulross characterizationofproinflammatoryflagellinproteinsproducedbylactobacillusruminisandrelatedmotilelactobacilli AT paulwotoole characterizationofproinflammatoryflagellinproteinsproducedbylactobacillusruminisandrelatedmotilelactobacilli
_version_	1718423777761558528

Characterization of pro-inflammatory flagellin proteins produced by Lactobacillus ruminis and related motile Lactobacilli.

Ejemplares similares