Draft genome and description of Merdibacter massiliensis gen.nov., sp. nov., a new bacterium genus isolated from the human ileum

Abstract We used phenotypic, genomic and phylogenetic information following the taxono-genomics approach to demonstrate that strain Marseille–P3254, isolated from an ileal sample of a 76-year old woman who underwent upper and lower digestive tract endoscopy for esophagitis and colonic polyp, is repr...

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Autores principales:	Hussein Anani, Rita Abou Abdallah, Nisrine Chelkha, Anthony Fontanini, Davide Ricaboni, Morgane Mailhe, Didier Raoult, Pierre-Edouard Fournier
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2a83d5823006456da4427b98f541472d
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spelling	oai:doaj.org-article:2a83d5823006456da4427b98f541472d2021-12-02T15:08:59ZDraft genome and description of Merdibacter massiliensis gen.nov., sp. nov., a new bacterium genus isolated from the human ileum10.1038/s41598-019-44343-82045-2322https://doaj.org/article/2a83d5823006456da4427b98f541472d2019-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-44343-8https://doaj.org/toc/2045-2322Abstract We used phenotypic, genomic and phylogenetic information following the taxono-genomics approach to demonstrate that strain Marseille–P3254, isolated from an ileal sample of a 76-year old woman who underwent upper and lower digestive tract endoscopy for esophagitis and colonic polyp, is representative of a novel bacterial genus within the family Erysipelotrichaceae in the phylum Firmicutes. It is an anaerobic Gram-negative bacterium without catalase and oxidase activities. The genome of strain Marseille–P3254 is 2,468,496-bp long with a 40.1% G + C content. This new bacterium is most closely related to Eubacterium dolichum, with which it shares 90.7% 16S rRNA sequence similarity. In addition, genomic comparison using the digital DNA–DNA hybridization and OrthoANI analyses between the novel organism and the E. dolichum type strain revealed identities of 25.2 and 68.91%, respectively. The major fatty acids were C16: 0, C18: 1n9 and C18: 0. Based on these data, we propose the creation of the new genus Merdibacter gen. nov., with strain Marseille-P3254T (=CSUR P3254 = DSM 103534) being the type strain of the new species Merdibacter massiliensis gen. nov., sp. nov.Hussein AnaniRita Abou AbdallahNisrine ChelkhaAnthony FontaniniDavide RicaboniMorgane MailheDidier RaoultPierre-Edouard FournierNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hussein Anani Rita Abou Abdallah Nisrine Chelkha Anthony Fontanini Davide Ricaboni Morgane Mailhe Didier Raoult Pierre-Edouard Fournier Draft genome and description of Merdibacter massiliensis gen.nov., sp. nov., a new bacterium genus isolated from the human ileum
description	Abstract We used phenotypic, genomic and phylogenetic information following the taxono-genomics approach to demonstrate that strain Marseille–P3254, isolated from an ileal sample of a 76-year old woman who underwent upper and lower digestive tract endoscopy for esophagitis and colonic polyp, is representative of a novel bacterial genus within the family Erysipelotrichaceae in the phylum Firmicutes. It is an anaerobic Gram-negative bacterium without catalase and oxidase activities. The genome of strain Marseille–P3254 is 2,468,496-bp long with a 40.1% G + C content. This new bacterium is most closely related to Eubacterium dolichum, with which it shares 90.7% 16S rRNA sequence similarity. In addition, genomic comparison using the digital DNA–DNA hybridization and OrthoANI analyses between the novel organism and the E. dolichum type strain revealed identities of 25.2 and 68.91%, respectively. The major fatty acids were C16: 0, C18: 1n9 and C18: 0. Based on these data, we propose the creation of the new genus Merdibacter gen. nov., with strain Marseille-P3254T (=CSUR P3254 = DSM 103534) being the type strain of the new species Merdibacter massiliensis gen. nov., sp. nov.
format	article
author	Hussein Anani Rita Abou Abdallah Nisrine Chelkha Anthony Fontanini Davide Ricaboni Morgane Mailhe Didier Raoult Pierre-Edouard Fournier
author_facet	Hussein Anani Rita Abou Abdallah Nisrine Chelkha Anthony Fontanini Davide Ricaboni Morgane Mailhe Didier Raoult Pierre-Edouard Fournier
author_sort	Hussein Anani
title	Draft genome and description of Merdibacter massiliensis gen.nov., sp. nov., a new bacterium genus isolated from the human ileum
title_short	Draft genome and description of Merdibacter massiliensis gen.nov., sp. nov., a new bacterium genus isolated from the human ileum
title_full	Draft genome and description of Merdibacter massiliensis gen.nov., sp. nov., a new bacterium genus isolated from the human ileum
title_fullStr	Draft genome and description of Merdibacter massiliensis gen.nov., sp. nov., a new bacterium genus isolated from the human ileum
title_full_unstemmed	Draft genome and description of Merdibacter massiliensis gen.nov., sp. nov., a new bacterium genus isolated from the human ileum
title_sort	draft genome and description of merdibacter massiliensis gen.nov., sp. nov., a new bacterium genus isolated from the human ileum
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/2a83d5823006456da4427b98f541472d
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Draft genome and description of Merdibacter massiliensis gen.nov., sp. nov., a new bacterium genus isolated from the human ileum

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