Small flexible automated system for monitoring Caenorhabditis elegans lifespan based on active vision and image processing techniques

Abstract Traditionally Caenorhabditis elegans lifespan assays are performed by manually inspecting nematodes with a dissection microscope, which involves daily counting of live/dead worms cultured in Petri plates for 21–25 days. This manual inspection requires the screening of hundreds of worms to e...

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Autores principales:	Joan Carles Puchalt, Antonio-José Sánchez-Salmerón, Eugenio Ivorra, Silvia Llopis, Roberto Martínez, Patricia Martorell
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2a86df4ef0c749cea7d37f250e6c4045
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spelling	oai:doaj.org-article:2a86df4ef0c749cea7d37f250e6c40452021-12-02T17:52:40ZSmall flexible automated system for monitoring Caenorhabditis elegans lifespan based on active vision and image processing techniques10.1038/s41598-021-91898-62045-2322https://doaj.org/article/2a86df4ef0c749cea7d37f250e6c40452021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91898-6https://doaj.org/toc/2045-2322Abstract Traditionally Caenorhabditis elegans lifespan assays are performed by manually inspecting nematodes with a dissection microscope, which involves daily counting of live/dead worms cultured in Petri plates for 21–25 days. This manual inspection requires the screening of hundreds of worms to ensure statistical robustness, and is therefore a time-consuming approach. In recent years, various automated artificial vision systems have been reported to increase the throughput, however they usually provide less accurate results than manual assays. The main problems identified when using these vision systems are the false positives and false negatives, which occur due to culture media changes, occluded zones, dirtiness or condensation of the Petri plates. In this work, we developed and described a new C. elegans monitoring machine, SiViS, which consists of a flexible and compact platform design to analyse C. elegans cultures using the standard Petri plates seeded with E. coli. Our system uses an active vision illumination technique and different image-processing pipelines for motion detection, both previously reported, providing a fully automated image processing pipeline. In addition, this study validated both these methods and the feasibility of the SiViS machine for lifespan experiments by comparing them with manual lifespan assays. Results demonstrated that the automated system yields consistent replicates (p-value log rank test 0.699), and there are no significant differences between automated system assays and traditionally manual assays (p-value 0.637). Finally, although we have focused on the use of SiViS in longevity assays, the system configuration is flexible and can, thus, be adapted to other C. elegans studies such as toxicity, mobility and behaviour.Joan Carles PuchaltAntonio-José Sánchez-SalmerónEugenio IvorraSilvia LlopisRoberto MartínezPatricia MartorellNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joan Carles Puchalt Antonio-José Sánchez-Salmerón Eugenio Ivorra Silvia Llopis Roberto Martínez Patricia Martorell Small flexible automated system for monitoring Caenorhabditis elegans lifespan based on active vision and image processing techniques
description	Abstract Traditionally Caenorhabditis elegans lifespan assays are performed by manually inspecting nematodes with a dissection microscope, which involves daily counting of live/dead worms cultured in Petri plates for 21–25 days. This manual inspection requires the screening of hundreds of worms to ensure statistical robustness, and is therefore a time-consuming approach. In recent years, various automated artificial vision systems have been reported to increase the throughput, however they usually provide less accurate results than manual assays. The main problems identified when using these vision systems are the false positives and false negatives, which occur due to culture media changes, occluded zones, dirtiness or condensation of the Petri plates. In this work, we developed and described a new C. elegans monitoring machine, SiViS, which consists of a flexible and compact platform design to analyse C. elegans cultures using the standard Petri plates seeded with E. coli. Our system uses an active vision illumination technique and different image-processing pipelines for motion detection, both previously reported, providing a fully automated image processing pipeline. In addition, this study validated both these methods and the feasibility of the SiViS machine for lifespan experiments by comparing them with manual lifespan assays. Results demonstrated that the automated system yields consistent replicates (p-value log rank test 0.699), and there are no significant differences between automated system assays and traditionally manual assays (p-value 0.637). Finally, although we have focused on the use of SiViS in longevity assays, the system configuration is flexible and can, thus, be adapted to other C. elegans studies such as toxicity, mobility and behaviour.
format	article
author	Joan Carles Puchalt Antonio-José Sánchez-Salmerón Eugenio Ivorra Silvia Llopis Roberto Martínez Patricia Martorell
author_facet	Joan Carles Puchalt Antonio-José Sánchez-Salmerón Eugenio Ivorra Silvia Llopis Roberto Martínez Patricia Martorell
author_sort	Joan Carles Puchalt
title	Small flexible automated system for monitoring Caenorhabditis elegans lifespan based on active vision and image processing techniques
title_short	Small flexible automated system for monitoring Caenorhabditis elegans lifespan based on active vision and image processing techniques
title_full	Small flexible automated system for monitoring Caenorhabditis elegans lifespan based on active vision and image processing techniques
title_fullStr	Small flexible automated system for monitoring Caenorhabditis elegans lifespan based on active vision and image processing techniques
title_full_unstemmed	Small flexible automated system for monitoring Caenorhabditis elegans lifespan based on active vision and image processing techniques
title_sort	small flexible automated system for monitoring caenorhabditis elegans lifespan based on active vision and image processing techniques
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2a86df4ef0c749cea7d37f250e6c4045
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Small flexible automated system for monitoring Caenorhabditis elegans lifespan based on active vision and image processing techniques

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