Palmitic acid promotes resistin-induced insulin resistance and inflammation in SH-SY5Y human neuroblastoma

Abstract Saturated fatty acids such as palmitic acid promote inflammation and insulin resistance in peripheral tissues, contrasting with the protective action of polyunsaturated fatty acids such docosahexaenoic acid. Palmitic acid effects have been in part attributed to its potential action through...

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Autores principales:	Hamza Amine, Yacir Benomar, Mohammed Taouis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2a88b9f24e2340e7ad0801e1b6948c0d
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spelling	oai:doaj.org-article:2a88b9f24e2340e7ad0801e1b6948c0d2021-12-02T13:30:11ZPalmitic acid promotes resistin-induced insulin resistance and inflammation in SH-SY5Y human neuroblastoma10.1038/s41598-021-85018-72045-2322https://doaj.org/article/2a88b9f24e2340e7ad0801e1b6948c0d2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85018-7https://doaj.org/toc/2045-2322Abstract Saturated fatty acids such as palmitic acid promote inflammation and insulin resistance in peripheral tissues, contrasting with the protective action of polyunsaturated fatty acids such docosahexaenoic acid. Palmitic acid effects have been in part attributed to its potential action through Toll-like receptor 4. Beside, resistin, an adipokine, also promotes inflammation and insulin resistance via TLR4. In the brain, palmitic acid and resistin trigger neuroinflammation and insulin resistance, but their link at the neuronal level is unknown. Using human SH-SY5Yneuroblastoma cell line we show that palmitic acid treatment impaired insulin-dependent Akt and Erk phosphorylation whereas DHA preserved insulin action. Palmitic acid up-regulated TLR4 as well as pro-inflammatory cytokines IL6 and TNFα contrasting with DHA effect. Similarly to palmitic acid, resistin treatment induced the up-regulation of IL6 and TNFα as well as NFκB activation. Importantly, palmitic acid potentiated the resistin-dependent NFkB activation whereas DHA abolished it. The recruitment of TLR4 to membrane lipid rafts was increased by palmitic acid treatment; this is concomitant with the augmentation of resistin-induced TLR4/MYD88/TIRAP complex formation mandatory for TLR4 signaling. In conclusion, palmitic acid increased TLR4 expression promoting resistin signaling through TLR4 up-regulation and its recruitment to membrane lipid rafts.Hamza AmineYacir BenomarMohammed TaouisNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hamza Amine Yacir Benomar Mohammed Taouis Palmitic acid promotes resistin-induced insulin resistance and inflammation in SH-SY5Y human neuroblastoma
description	Abstract Saturated fatty acids such as palmitic acid promote inflammation and insulin resistance in peripheral tissues, contrasting with the protective action of polyunsaturated fatty acids such docosahexaenoic acid. Palmitic acid effects have been in part attributed to its potential action through Toll-like receptor 4. Beside, resistin, an adipokine, also promotes inflammation and insulin resistance via TLR4. In the brain, palmitic acid and resistin trigger neuroinflammation and insulin resistance, but their link at the neuronal level is unknown. Using human SH-SY5Yneuroblastoma cell line we show that palmitic acid treatment impaired insulin-dependent Akt and Erk phosphorylation whereas DHA preserved insulin action. Palmitic acid up-regulated TLR4 as well as pro-inflammatory cytokines IL6 and TNFα contrasting with DHA effect. Similarly to palmitic acid, resistin treatment induced the up-regulation of IL6 and TNFα as well as NFκB activation. Importantly, palmitic acid potentiated the resistin-dependent NFkB activation whereas DHA abolished it. The recruitment of TLR4 to membrane lipid rafts was increased by palmitic acid treatment; this is concomitant with the augmentation of resistin-induced TLR4/MYD88/TIRAP complex formation mandatory for TLR4 signaling. In conclusion, palmitic acid increased TLR4 expression promoting resistin signaling through TLR4 up-regulation and its recruitment to membrane lipid rafts.
format	article
author	Hamza Amine Yacir Benomar Mohammed Taouis
author_facet	Hamza Amine Yacir Benomar Mohammed Taouis
author_sort	Hamza Amine
title	Palmitic acid promotes resistin-induced insulin resistance and inflammation in SH-SY5Y human neuroblastoma
title_short	Palmitic acid promotes resistin-induced insulin resistance and inflammation in SH-SY5Y human neuroblastoma
title_full	Palmitic acid promotes resistin-induced insulin resistance and inflammation in SH-SY5Y human neuroblastoma
title_fullStr	Palmitic acid promotes resistin-induced insulin resistance and inflammation in SH-SY5Y human neuroblastoma
title_full_unstemmed	Palmitic acid promotes resistin-induced insulin resistance and inflammation in SH-SY5Y human neuroblastoma
title_sort	palmitic acid promotes resistin-induced insulin resistance and inflammation in sh-sy5y human neuroblastoma
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2a88b9f24e2340e7ad0801e1b6948c0d
work_keys_str_mv	AT hamzaamine palmiticacidpromotesresistininducedinsulinresistanceandinflammationinshsy5yhumanneuroblastoma AT yacirbenomar palmiticacidpromotesresistininducedinsulinresistanceandinflammationinshsy5yhumanneuroblastoma AT mohammedtaouis palmiticacidpromotesresistininducedinsulinresistanceandinflammationinshsy5yhumanneuroblastoma
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Palmitic acid promotes resistin-induced insulin resistance and inflammation in SH-SY5Y human neuroblastoma

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