Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources

Thirty bacterial isolates were tested on three different media for Poly-ß-hydroxybutyrate (PHB) production. The best bacterial isolates for producing PHB were screened and identified based on molecular biology; then, using three different alternative carbon sources (dried whey, sugar beet molasses a...

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Autores principales:	Sherif M. El-Kadi, Mohssen Elbagory, Hassan A. H. EL-Zawawy, Hossam F. A. EL-Shaer, Adel A. Shoukry, Sahar El-Nahrawy, Alaa El-Dein Omara, Dina Fathi Ismail Ali
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	agro-industrial waste GC-MS/MS spectrometry IR spectrometry PHB Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/2a8b7c7448754c699e5322bef41d8a1c
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spelling	oai:doaj.org-article:2a8b7c7448754c699e5322bef41d8a1c2021-11-11T18:47:42ZBiosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources10.3390/polym132138012073-4360https://doaj.org/article/2a8b7c7448754c699e5322bef41d8a1c2021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/21/3801https://doaj.org/toc/2073-4360Thirty bacterial isolates were tested on three different media for Poly-ß-hydroxybutyrate (PHB) production. The best bacterial isolates for producing PHB were screened and identified based on molecular biology; then, using three different alternative carbon sources (dried whey, sugar beet molasses and date molasses), physical properties were evaluated by Infrared (IR) spectrometry and Gas chromatography–mass spectrometry (GC-MS/MS) analysis. Our results showed that the best isolates identified based on molecular biology were <i>Bacillus paramycoides</i> MCCC 1A04098, <i>Azotobacter salinestris</i> NBRC 102611 and <i>Brevundimonas naejangsanensis</i> BIO-TAS2-2. The addition of sugar beet molasses to the medium of <i>A. salinestris</i> increased the cell dry weight (CDW), PHB concentration, PHB% and conversion coefficient (4.97 g/L, 1.56 g/L, 31.38% and 23.92%, respectively). The correlation coefficient values between PHB g/L and CDW g/L varied between very strong and moderate positive correlation. IR of the produced PHB from <i>B. paramycoides</i> and <i>A. salinestris</i> showed similar bands which confirmed the presence of PHB; however, <i>B. naejangsanensis</i> showed weak bands, indicating lower PHB concentration. The chemical composition obtained showed that the GC-MS of the PHB extracted represents 2, 4-ditert-butylphenol for <i>B. paramycoides</i> and isopropyl ester of 2-butenoic acid for both of <i>A. salinestris</i> and <i>Brevundimonas naejangsanensis</i>. Therefore, PHB produced by microorganisms can be considered a biodegradable polyester, and represents a promising technique for the development of eco-friendly and fully biodegradable plastics.Sherif M. El-KadiMohssen ElbagoryHassan A. H. EL-ZawawyHossam F. A. EL-ShaerAdel A. ShoukrySahar El-NahrawyAlaa El-Dein OmaraDina Fathi Ismail AliMDPI AGarticleagro-industrial wasteGC-MS/MS spectrometryIR spectrometryPHBOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3801, p 3801 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	agro-industrial waste GC-MS/MS spectrometry IR spectrometry PHB Organic chemistry QD241-441
spellingShingle	agro-industrial waste GC-MS/MS spectrometry IR spectrometry PHB Organic chemistry QD241-441 Sherif M. El-Kadi Mohssen Elbagory Hassan A. H. EL-Zawawy Hossam F. A. EL-Shaer Adel A. Shoukry Sahar El-Nahrawy Alaa El-Dein Omara Dina Fathi Ismail Ali Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources
description	Thirty bacterial isolates were tested on three different media for Poly-ß-hydroxybutyrate (PHB) production. The best bacterial isolates for producing PHB were screened and identified based on molecular biology; then, using three different alternative carbon sources (dried whey, sugar beet molasses and date molasses), physical properties were evaluated by Infrared (IR) spectrometry and Gas chromatography–mass spectrometry (GC-MS/MS) analysis. Our results showed that the best isolates identified based on molecular biology were <i>Bacillus paramycoides</i> MCCC 1A04098, <i>Azotobacter salinestris</i> NBRC 102611 and <i>Brevundimonas naejangsanensis</i> BIO-TAS2-2. The addition of sugar beet molasses to the medium of <i>A. salinestris</i> increased the cell dry weight (CDW), PHB concentration, PHB% and conversion coefficient (4.97 g/L, 1.56 g/L, 31.38% and 23.92%, respectively). The correlation coefficient values between PHB g/L and CDW g/L varied between very strong and moderate positive correlation. IR of the produced PHB from <i>B. paramycoides</i> and <i>A. salinestris</i> showed similar bands which confirmed the presence of PHB; however, <i>B. naejangsanensis</i> showed weak bands, indicating lower PHB concentration. The chemical composition obtained showed that the GC-MS of the PHB extracted represents 2, 4-ditert-butylphenol for <i>B. paramycoides</i> and isopropyl ester of 2-butenoic acid for both of <i>A. salinestris</i> and <i>Brevundimonas naejangsanensis</i>. Therefore, PHB produced by microorganisms can be considered a biodegradable polyester, and represents a promising technique for the development of eco-friendly and fully biodegradable plastics.
format	article
author	Sherif M. El-Kadi Mohssen Elbagory Hassan A. H. EL-Zawawy Hossam F. A. EL-Shaer Adel A. Shoukry Sahar El-Nahrawy Alaa El-Dein Omara Dina Fathi Ismail Ali
author_facet	Sherif M. El-Kadi Mohssen Elbagory Hassan A. H. EL-Zawawy Hossam F. A. EL-Shaer Adel A. Shoukry Sahar El-Nahrawy Alaa El-Dein Omara Dina Fathi Ismail Ali
author_sort	Sherif M. El-Kadi
title	Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources
title_short	Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources
title_full	Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources
title_fullStr	Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources
title_full_unstemmed	Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources
title_sort	biosynthesis of poly-ß-hydroxybutyrate (phb) from different bacterial strains grown on alternative cheap carbon sources
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2a8b7c7448754c699e5322bef41d8a1c
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Biosynthesis of Poly-ß-Hydroxybutyrate (PHB) from Different Bacterial Strains Grown on Alternative Cheap Carbon Sources

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