Super-resolution microscopy with very large working distance by means of distributed aperture illumination

Abstract The limits of conventional light microscopy (“Abbe-Limit“) depend critically on the numerical aperture (NA) of the objective lens. Imaging at large working distances or a large field-of-view typically requires low NA objectives, thereby reducing the optical resolution to the multi micromete...

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Autores principales:	Udo Birk, Johann v. Hase, Christoph Cremer
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2a99d9711627477483bb0270072bd9e7
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spelling	oai:doaj.org-article:2a99d9711627477483bb0270072bd9e72021-12-02T12:32:06ZSuper-resolution microscopy with very large working distance by means of distributed aperture illumination10.1038/s41598-017-03743-42045-2322https://doaj.org/article/2a99d9711627477483bb0270072bd9e72017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03743-4https://doaj.org/toc/2045-2322Abstract The limits of conventional light microscopy (“Abbe-Limit“) depend critically on the numerical aperture (NA) of the objective lens. Imaging at large working distances or a large field-of-view typically requires low NA objectives, thereby reducing the optical resolution to the multi micrometer range. Based on numerical simulations of the intensity field distribution, we present an illumination concept for a super-resolution microscope which allows a three dimensional (3D) optical resolution around 150 nm for working distances up to the centimeter regime. In principle, the system allows great flexibility, because the illumination concept can be used to approximate the point-spread-function of conventional microscope optics, with the additional benefit of a customizable pupil function. Compared with the Abbe-limit using an objective lens with such a large working distance, a volume resolution enhancement potential in the order of 104 is estimated.Udo BirkJohann v. HaseChristoph CremerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Udo Birk Johann v. Hase Christoph Cremer Super-resolution microscopy with very large working distance by means of distributed aperture illumination
description	Abstract The limits of conventional light microscopy (“Abbe-Limit“) depend critically on the numerical aperture (NA) of the objective lens. Imaging at large working distances or a large field-of-view typically requires low NA objectives, thereby reducing the optical resolution to the multi micrometer range. Based on numerical simulations of the intensity field distribution, we present an illumination concept for a super-resolution microscope which allows a three dimensional (3D) optical resolution around 150 nm for working distances up to the centimeter regime. In principle, the system allows great flexibility, because the illumination concept can be used to approximate the point-spread-function of conventional microscope optics, with the additional benefit of a customizable pupil function. Compared with the Abbe-limit using an objective lens with such a large working distance, a volume resolution enhancement potential in the order of 104 is estimated.
format	article
author	Udo Birk Johann v. Hase Christoph Cremer
author_facet	Udo Birk Johann v. Hase Christoph Cremer
author_sort	Udo Birk
title	Super-resolution microscopy with very large working distance by means of distributed aperture illumination
title_short	Super-resolution microscopy with very large working distance by means of distributed aperture illumination
title_full	Super-resolution microscopy with very large working distance by means of distributed aperture illumination
title_fullStr	Super-resolution microscopy with very large working distance by means of distributed aperture illumination
title_full_unstemmed	Super-resolution microscopy with very large working distance by means of distributed aperture illumination
title_sort	super-resolution microscopy with very large working distance by means of distributed aperture illumination
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2a99d9711627477483bb0270072bd9e7
work_keys_str_mv	AT udobirk superresolutionmicroscopywithverylargeworkingdistancebymeansofdistributedapertureillumination AT johannvhase superresolutionmicroscopywithverylargeworkingdistancebymeansofdistributedapertureillumination AT christophcremer superresolutionmicroscopywithverylargeworkingdistancebymeansofdistributedapertureillumination
_version_	1718394146210709504

Super-resolution microscopy with very large working distance by means of distributed aperture illumination

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