Tandem mass spectrometry of aqueous extract from Ficus dubia sap and its cell-based assessments for use as a skin antioxidant

Abstract Since 2006, Ficus dubia has been reported as a new Ficus species in Thailand. As per our recent report, the red-brown aqueous extract of F. dubia sap (FDS) has been determined to strongly exhibit in vitro anti-radicals. However, the phytochemicals in the FDS extract related to health-promot...

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Autores principales:	Chaisak Chansriniyom, Rawiwan Nooin, Nitra Nuengchamnong, Ratjika Wongwanakul, Nalinrat Petpiroon, Wanwisa Srinuanchai, Bhanumas Chantarasuwan, Pornsiri Pitchakarn, Piya Temviriyanukul, Onanong Nuchuchua
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2a9df8841b824cb585e330f6cdac28a1
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spelling	oai:doaj.org-article:2a9df8841b824cb585e330f6cdac28a12021-12-02T17:08:36ZTandem mass spectrometry of aqueous extract from Ficus dubia sap and its cell-based assessments for use as a skin antioxidant10.1038/s41598-021-96261-32045-2322https://doaj.org/article/2a9df8841b824cb585e330f6cdac28a12021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96261-3https://doaj.org/toc/2045-2322Abstract Since 2006, Ficus dubia has been reported as a new Ficus species in Thailand. As per our recent report, the red-brown aqueous extract of F. dubia sap (FDS) has been determined to strongly exhibit in vitro anti-radicals. However, the phytochemicals in the FDS extract related to health-promoting antioxidation have not been explored. Thus, in this study, we aimed to investigate the chemical components of the F. dubia sap extract by liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC–ESI–MS/QTOF-MS) and its potential use in cosmetics in terms of cellular antioxidation on keratinocytes (HaCaT), phototoxicity, and irritation on 3D skin cell models following standard tests suggested by the Organization for Economic Cooperation and Development (OECD). It was found that the sap extract was composed of quinic acid and caffeoyl derivatives (e.g., syringoylquinic acid, 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, and dimeric forms of caffeoylquinic acids). The extract has significantly exhibited antioxidant activity against H2O2-induced oxidative stress in HaCaT cells. The cellular antioxidative effect of the FDS extract was remarkably dependent on the presence of 3- and 4-O-caffeoylquinic acid in the extract. Furthermore, the FDS extract showed negative results on skin phototoxicity and irritation. Overall, the results reveal that the FDS extract could be developed as a new antioxidant candidate for a skin healthcare product.Chaisak ChansriniyomRawiwan NooinNitra NuengchamnongRatjika WongwanakulNalinrat PetpiroonWanwisa SrinuanchaiBhanumas ChantarasuwanPornsiri PitchakarnPiya TemviriyanukulOnanong NuchuchuaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chaisak Chansriniyom Rawiwan Nooin Nitra Nuengchamnong Ratjika Wongwanakul Nalinrat Petpiroon Wanwisa Srinuanchai Bhanumas Chantarasuwan Pornsiri Pitchakarn Piya Temviriyanukul Onanong Nuchuchua Tandem mass spectrometry of aqueous extract from Ficus dubia sap and its cell-based assessments for use as a skin antioxidant
description	Abstract Since 2006, Ficus dubia has been reported as a new Ficus species in Thailand. As per our recent report, the red-brown aqueous extract of F. dubia sap (FDS) has been determined to strongly exhibit in vitro anti-radicals. However, the phytochemicals in the FDS extract related to health-promoting antioxidation have not been explored. Thus, in this study, we aimed to investigate the chemical components of the F. dubia sap extract by liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC–ESI–MS/QTOF-MS) and its potential use in cosmetics in terms of cellular antioxidation on keratinocytes (HaCaT), phototoxicity, and irritation on 3D skin cell models following standard tests suggested by the Organization for Economic Cooperation and Development (OECD). It was found that the sap extract was composed of quinic acid and caffeoyl derivatives (e.g., syringoylquinic acid, 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, and dimeric forms of caffeoylquinic acids). The extract has significantly exhibited antioxidant activity against H2O2-induced oxidative stress in HaCaT cells. The cellular antioxidative effect of the FDS extract was remarkably dependent on the presence of 3- and 4-O-caffeoylquinic acid in the extract. Furthermore, the FDS extract showed negative results on skin phototoxicity and irritation. Overall, the results reveal that the FDS extract could be developed as a new antioxidant candidate for a skin healthcare product.
format	article
author	Chaisak Chansriniyom Rawiwan Nooin Nitra Nuengchamnong Ratjika Wongwanakul Nalinrat Petpiroon Wanwisa Srinuanchai Bhanumas Chantarasuwan Pornsiri Pitchakarn Piya Temviriyanukul Onanong Nuchuchua
author_facet	Chaisak Chansriniyom Rawiwan Nooin Nitra Nuengchamnong Ratjika Wongwanakul Nalinrat Petpiroon Wanwisa Srinuanchai Bhanumas Chantarasuwan Pornsiri Pitchakarn Piya Temviriyanukul Onanong Nuchuchua
author_sort	Chaisak Chansriniyom
title	Tandem mass spectrometry of aqueous extract from Ficus dubia sap and its cell-based assessments for use as a skin antioxidant
title_short	Tandem mass spectrometry of aqueous extract from Ficus dubia sap and its cell-based assessments for use as a skin antioxidant
title_full	Tandem mass spectrometry of aqueous extract from Ficus dubia sap and its cell-based assessments for use as a skin antioxidant
title_fullStr	Tandem mass spectrometry of aqueous extract from Ficus dubia sap and its cell-based assessments for use as a skin antioxidant
title_full_unstemmed	Tandem mass spectrometry of aqueous extract from Ficus dubia sap and its cell-based assessments for use as a skin antioxidant
title_sort	tandem mass spectrometry of aqueous extract from ficus dubia sap and its cell-based assessments for use as a skin antioxidant
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2a9df8841b824cb585e330f6cdac28a1
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Tandem mass spectrometry of aqueous extract from Ficus dubia sap and its cell-based assessments for use as a skin antioxidant

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