Short-term transport stress and supplementation alter immune function in aged horses.

Long-distance transport is associated with stress-related changes in equine immune function, and shipping-associated illnesses are often reported. Horses are frequently transported short distances, yet the effects of short-term transport on immune function remain largely unknown. Twelve horses, aged...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ashton B Miller, Patricia A Harris, Virginia D Barker, Amanda A Adams
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2a9f375060a04310af279c1d57333a3d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2a9f375060a04310af279c1d57333a3d
record_format	dspace
spelling	oai:doaj.org-article:2a9f375060a04310af279c1d57333a3d2021-12-02T20:14:58ZShort-term transport stress and supplementation alter immune function in aged horses.1932-620310.1371/journal.pone.0254139https://doaj.org/article/2a9f375060a04310af279c1d57333a3d2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254139https://doaj.org/toc/1932-6203Long-distance transport is associated with stress-related changes in equine immune function, and shipping-associated illnesses are often reported. Horses are frequently transported short distances, yet the effects of short-term transport on immune function remain largely unknown. Twelve horses, aged 15-30 yr, were assigned to either the control (n = 6) or treatment (n = 6) groups; treatment horses received a daily antioxidant supplement 3 weeks before and after transport. All horses were transported for approximately 1.5-2 hr on Day 0. Blood was collected via jugular venipuncture at 15-min pre- and post-transport and on Days -21, 1, 3, 7, 14, and 21. Body temperature, heart rate, body weight, total cortisol, and gene expression of IFNγ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12α, IL-17α, SAA1, and TNFα in whole blood were measured. Peripheral blood mononuclear cells were isolated, stimulated with PMA/ionomycin, and stained for IFNγ and TNFα before analysis via flow cytometry. Statistical analyses were performed with significance set at P < 0.05 (SAS 9.4). Transport and supplementation did not appear to affect body weight, heart rate, IL-4, IL-8, IL-12α, IL-17α, change (Δ) in the % and mean fluorescence intensity (MFI) of IFNγ+ lymphocytes after stimulation, or Δ in the % and MFI of TNFα+ lymphocytes after stimulation. Supplementation decreased IL-1β and SAA1 expression. Transport increased total cortisol concentration, body temperature, and IL-2, IL-6, and IL-10 expression but decreased IL-1β, TNFα, and IFNγ expression. Short-term transportation affected physiological, endocrine, and immune responses; supplementation may ameliorate inflammation in aged horses. Immune responses were most altered at 15-min post-transport and typically recovered by Day 1, suggesting that horses may be vulnerable to disease during and almost immediately after short-term transport.Ashton B MillerPatricia A HarrisVirginia D BarkerAmanda A AdamsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0254139 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ashton B Miller Patricia A Harris Virginia D Barker Amanda A Adams Short-term transport stress and supplementation alter immune function in aged horses.
description	Long-distance transport is associated with stress-related changes in equine immune function, and shipping-associated illnesses are often reported. Horses are frequently transported short distances, yet the effects of short-term transport on immune function remain largely unknown. Twelve horses, aged 15-30 yr, were assigned to either the control (n = 6) or treatment (n = 6) groups; treatment horses received a daily antioxidant supplement 3 weeks before and after transport. All horses were transported for approximately 1.5-2 hr on Day 0. Blood was collected via jugular venipuncture at 15-min pre- and post-transport and on Days -21, 1, 3, 7, 14, and 21. Body temperature, heart rate, body weight, total cortisol, and gene expression of IFNγ, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12α, IL-17α, SAA1, and TNFα in whole blood were measured. Peripheral blood mononuclear cells were isolated, stimulated with PMA/ionomycin, and stained for IFNγ and TNFα before analysis via flow cytometry. Statistical analyses were performed with significance set at P < 0.05 (SAS 9.4). Transport and supplementation did not appear to affect body weight, heart rate, IL-4, IL-8, IL-12α, IL-17α, change (Δ) in the % and mean fluorescence intensity (MFI) of IFNγ+ lymphocytes after stimulation, or Δ in the % and MFI of TNFα+ lymphocytes after stimulation. Supplementation decreased IL-1β and SAA1 expression. Transport increased total cortisol concentration, body temperature, and IL-2, IL-6, and IL-10 expression but decreased IL-1β, TNFα, and IFNγ expression. Short-term transportation affected physiological, endocrine, and immune responses; supplementation may ameliorate inflammation in aged horses. Immune responses were most altered at 15-min post-transport and typically recovered by Day 1, suggesting that horses may be vulnerable to disease during and almost immediately after short-term transport.
format	article
author	Ashton B Miller Patricia A Harris Virginia D Barker Amanda A Adams
author_facet	Ashton B Miller Patricia A Harris Virginia D Barker Amanda A Adams
author_sort	Ashton B Miller
title	Short-term transport stress and supplementation alter immune function in aged horses.
title_short	Short-term transport stress and supplementation alter immune function in aged horses.
title_full	Short-term transport stress and supplementation alter immune function in aged horses.
title_fullStr	Short-term transport stress and supplementation alter immune function in aged horses.
title_full_unstemmed	Short-term transport stress and supplementation alter immune function in aged horses.
title_sort	short-term transport stress and supplementation alter immune function in aged horses.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/2a9f375060a04310af279c1d57333a3d
work_keys_str_mv	AT ashtonbmiller shorttermtransportstressandsupplementationalterimmunefunctioninagedhorses AT patriciaaharris shorttermtransportstressandsupplementationalterimmunefunctioninagedhorses AT virginiadbarker shorttermtransportstressandsupplementationalterimmunefunctioninagedhorses AT amandaaadams shorttermtransportstressandsupplementationalterimmunefunctioninagedhorses
_version_	1718374634990075904

Short-term transport stress and supplementation alter immune function in aged horses.

Ejemplares similares