Impact of Antigen Density on the Binding Mechanism of IgG Antibodies

Abstract The density and distribution pattern of epitopes at the surface of pathogens have a profound impact on immune responses. Although multiple lines of evidence highlight the significance of antigen surface density for antibody binding, a quantitative description of its effect on recognition me...

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Autores principales:	Maya Hadzhieva, Anastas D. Pashov, Srinivas Kaveri, Sébastien Lacroix-Desmazes, Hugo Mouquet, Jordan D. Dimitrov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2a9f6eab595e44edb829b92bb61362e7
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spelling	oai:doaj.org-article:2a9f6eab595e44edb829b92bb61362e72021-12-02T11:40:32ZImpact of Antigen Density on the Binding Mechanism of IgG Antibodies10.1038/s41598-017-03942-z2045-2322https://doaj.org/article/2a9f6eab595e44edb829b92bb61362e72017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03942-zhttps://doaj.org/toc/2045-2322Abstract The density and distribution pattern of epitopes at the surface of pathogens have a profound impact on immune responses. Although multiple lines of evidence highlight the significance of antigen surface density for antibody binding, a quantitative description of its effect on recognition mechanisms is missing. Here, we analyzed binding kinetics and thermodynamics of six HIV-1 neutralizing antibodies as a function of the surface density of envelope glycoprotein gp120. Antibodies that recognize gp120 with low to moderate binding affinity displayed the most pronounced sensitivity to variation in antigen density, with qualitative and substantial quantitative changes in the energetics of the binding process as revealed by non-equilibrium and equilibrium thermodynamic analyses. In contrast, the recognition of gp120 by the antibodies with the highest affinity was considerably less influenced by variations in antigen density. These data suggest that a lower affinity of antibodies permits higher dynamics during the antigen recognition process, which may have considerable functional repercussions. These findings contribute to a better understanding of the mechanisms of antigen recognition by antibodies. They are also of importance for apprehending the impact of antigen topology on immune-defense functions of antibodies.Maya HadzhievaAnastas D. PashovSrinivas KaveriSébastien Lacroix-DesmazesHugo MouquetJordan D. DimitrovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Maya Hadzhieva Anastas D. Pashov Srinivas Kaveri Sébastien Lacroix-Desmazes Hugo Mouquet Jordan D. Dimitrov Impact of Antigen Density on the Binding Mechanism of IgG Antibodies
description	Abstract The density and distribution pattern of epitopes at the surface of pathogens have a profound impact on immune responses. Although multiple lines of evidence highlight the significance of antigen surface density for antibody binding, a quantitative description of its effect on recognition mechanisms is missing. Here, we analyzed binding kinetics and thermodynamics of six HIV-1 neutralizing antibodies as a function of the surface density of envelope glycoprotein gp120. Antibodies that recognize gp120 with low to moderate binding affinity displayed the most pronounced sensitivity to variation in antigen density, with qualitative and substantial quantitative changes in the energetics of the binding process as revealed by non-equilibrium and equilibrium thermodynamic analyses. In contrast, the recognition of gp120 by the antibodies with the highest affinity was considerably less influenced by variations in antigen density. These data suggest that a lower affinity of antibodies permits higher dynamics during the antigen recognition process, which may have considerable functional repercussions. These findings contribute to a better understanding of the mechanisms of antigen recognition by antibodies. They are also of importance for apprehending the impact of antigen topology on immune-defense functions of antibodies.
format	article
author	Maya Hadzhieva Anastas D. Pashov Srinivas Kaveri Sébastien Lacroix-Desmazes Hugo Mouquet Jordan D. Dimitrov
author_facet	Maya Hadzhieva Anastas D. Pashov Srinivas Kaveri Sébastien Lacroix-Desmazes Hugo Mouquet Jordan D. Dimitrov
author_sort	Maya Hadzhieva
title	Impact of Antigen Density on the Binding Mechanism of IgG Antibodies
title_short	Impact of Antigen Density on the Binding Mechanism of IgG Antibodies
title_full	Impact of Antigen Density on the Binding Mechanism of IgG Antibodies
title_fullStr	Impact of Antigen Density on the Binding Mechanism of IgG Antibodies
title_full_unstemmed	Impact of Antigen Density on the Binding Mechanism of IgG Antibodies
title_sort	impact of antigen density on the binding mechanism of igg antibodies
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2a9f6eab595e44edb829b92bb61362e7
work_keys_str_mv	AT mayahadzhieva impactofantigendensityonthebindingmechanismofiggantibodies AT anastasdpashov impactofantigendensityonthebindingmechanismofiggantibodies AT srinivaskaveri impactofantigendensityonthebindingmechanismofiggantibodies AT sebastienlacroixdesmazes impactofantigendensityonthebindingmechanismofiggantibodies AT hugomouquet impactofantigendensityonthebindingmechanismofiggantibodies AT jordanddimitrov impactofantigendensityonthebindingmechanismofiggantibodies
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Impact of Antigen Density on the Binding Mechanism of IgG Antibodies

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