Gremlin is overexpressed in lung adenocarcinoma and increases cell growth and proliferation in normal lung cells.

<h4>Background</h4>Gremlin, a member of the Dan family of BMP antagonists, is a glycosylated extracellular protein. Previously Gremlin has been shown to play a role in dorsal-ventral patterning, in tissue remodeling, and recently in angiogenesis. Evidence has previously been presented sh...

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Autores principales:	Michael S Mulvihill, Yong-Won Kwon, Sharon Lee, Li Tai Fang, Helen Choi, Roshni Ray, Hio Chung Kang, Jian-Hua Mao, David Jablons, Il-Jin Kim
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2aa363f00c8e44328863f830fc73c251
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spelling	oai:doaj.org-article:2aa363f00c8e44328863f830fc73c2512021-11-18T07:10:04ZGremlin is overexpressed in lung adenocarcinoma and increases cell growth and proliferation in normal lung cells.1932-620310.1371/journal.pone.0042264https://doaj.org/article/2aa363f00c8e44328863f830fc73c2512012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22870311/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Gremlin, a member of the Dan family of BMP antagonists, is a glycosylated extracellular protein. Previously Gremlin has been shown to play a role in dorsal-ventral patterning, in tissue remodeling, and recently in angiogenesis. Evidence has previously been presented showing both over- and under-expression of Gremlin in different tumor tissues. Here, we sought to quantify expression of Gremlin in cancers of the lung and performed in vitro experiments to check whether Gremlin promotes cell growth and proliferation.<h4>Methodology/principal findings</h4>Expression of Gremlin in 161 matched tumor and normal lung cancer specimens is quantified by quantitative real-time PCR and protein level is measured by immunohistochemistry. GREM1 was transfected into lung fibroblast and epithelial cell lines to assess the impact of overexpression of Gremlin in vitro.<h4>Results</h4>Lung adenocarcinoma but not squamous cell carcinoma shows a significant increase in Gremlin expression by mRNA and protein level. Lung fibroblast and epithelial cell lines transfected with GREM1 show significantly increased cell proliferation.<h4>Conclusions/significance</h4>Our data suggest that Gremlin acts in an oncogenic manner in lung adenocarcinoma and could hold promise as a new diagnostic marker or potential therapeutic target in lung AD or general thoracic malignancies.Michael S MulvihillYong-Won KwonSharon LeeLi Tai FangHelen ChoiRoshni RayHio Chung KangJian-Hua MaoDavid JablonsIl-Jin KimPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42264 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michael S Mulvihill Yong-Won Kwon Sharon Lee Li Tai Fang Helen Choi Roshni Ray Hio Chung Kang Jian-Hua Mao David Jablons Il-Jin Kim Gremlin is overexpressed in lung adenocarcinoma and increases cell growth and proliferation in normal lung cells.
description	<h4>Background</h4>Gremlin, a member of the Dan family of BMP antagonists, is a glycosylated extracellular protein. Previously Gremlin has been shown to play a role in dorsal-ventral patterning, in tissue remodeling, and recently in angiogenesis. Evidence has previously been presented showing both over- and under-expression of Gremlin in different tumor tissues. Here, we sought to quantify expression of Gremlin in cancers of the lung and performed in vitro experiments to check whether Gremlin promotes cell growth and proliferation.<h4>Methodology/principal findings</h4>Expression of Gremlin in 161 matched tumor and normal lung cancer specimens is quantified by quantitative real-time PCR and protein level is measured by immunohistochemistry. GREM1 was transfected into lung fibroblast and epithelial cell lines to assess the impact of overexpression of Gremlin in vitro.<h4>Results</h4>Lung adenocarcinoma but not squamous cell carcinoma shows a significant increase in Gremlin expression by mRNA and protein level. Lung fibroblast and epithelial cell lines transfected with GREM1 show significantly increased cell proliferation.<h4>Conclusions/significance</h4>Our data suggest that Gremlin acts in an oncogenic manner in lung adenocarcinoma and could hold promise as a new diagnostic marker or potential therapeutic target in lung AD or general thoracic malignancies.
format	article
author	Michael S Mulvihill Yong-Won Kwon Sharon Lee Li Tai Fang Helen Choi Roshni Ray Hio Chung Kang Jian-Hua Mao David Jablons Il-Jin Kim
author_facet	Michael S Mulvihill Yong-Won Kwon Sharon Lee Li Tai Fang Helen Choi Roshni Ray Hio Chung Kang Jian-Hua Mao David Jablons Il-Jin Kim
author_sort	Michael S Mulvihill
title	Gremlin is overexpressed in lung adenocarcinoma and increases cell growth and proliferation in normal lung cells.
title_short	Gremlin is overexpressed in lung adenocarcinoma and increases cell growth and proliferation in normal lung cells.
title_full	Gremlin is overexpressed in lung adenocarcinoma and increases cell growth and proliferation in normal lung cells.
title_fullStr	Gremlin is overexpressed in lung adenocarcinoma and increases cell growth and proliferation in normal lung cells.
title_full_unstemmed	Gremlin is overexpressed in lung adenocarcinoma and increases cell growth and proliferation in normal lung cells.
title_sort	gremlin is overexpressed in lung adenocarcinoma and increases cell growth and proliferation in normal lung cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2aa363f00c8e44328863f830fc73c251
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Gremlin is overexpressed in lung adenocarcinoma and increases cell growth and proliferation in normal lung cells.

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