Cu transporter protein CrpF protects against Cu-induced toxicity in Fusarium oxysporum

Cu is an essential trace element for cell growth and proliferation. However, excess of Cu accumulation leads to cellular toxicity. Thus, precise and tight regulation of Cu homeostasis processes, including transport, delivery, storage, detoxification, and efflux machineries, is required. Moreover, th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Damaris Lorenzo-Gutiérrez, Lucía Gómez-Gil, Josep Guarro, M. Isabel G. Roncero, Javier Capilla, Loida López-Fernández
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	copper (cu) transport cu homeostasis fungal pathogenesis pib-type atpase crp Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/2aa5659f50f14c189e867443002abfcf
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2aa5659f50f14c189e867443002abfcf
record_format	dspace
spelling	oai:doaj.org-article:2aa5659f50f14c189e867443002abfcf2021-11-17T14:21:58ZCu transporter protein CrpF protects against Cu-induced toxicity in Fusarium oxysporum2150-55942150-560810.1080/21505594.2020.1809324https://doaj.org/article/2aa5659f50f14c189e867443002abfcf2020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1809324https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Cu is an essential trace element for cell growth and proliferation. However, excess of Cu accumulation leads to cellular toxicity. Thus, precise and tight regulation of Cu homeostasis processes, including transport, delivery, storage, detoxification, and efflux machineries, is required. Moreover, the maintenance of Cu homeostasis is critical for the survival and virulence of fungal pathogens. Cu homeostasis has been extensively studied in mammals, bacteria, and yeast, but it has not yet been well documented in filamentous fungi. In the present work, we investigated Cu tolerance in the filamentous fungus Fusarium oxysporum by analysing the Cu transporter coding gene crpF, previously studied in Aspergillus fumigatus. The expression studies demonstrated that crpF is upregulated in the presence of Cu and its deletion leads to severe sensitivity to low levels of CuSO4 in F. oxysporum. Targeted deletion of crpF did not significantly alter the resistance of the fungus to macrophage killing, nor its pathogenic behaviour on the tomato plants. However, the targeted deletion mutant ΔcrpF showed increased virulence in a murine model of systemic infection compared to wild-type strain (wt).Damaris Lorenzo-GutiérrezLucía Gómez-GilJosep GuarroM. Isabel G. RonceroJavier CapillaLoida López-FernándezTaylor & Francis Grouparticlecopper (cu) transportcu homeostasisfungal pathogenesispib-type atpasecrpInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 1108-1121 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	copper (cu) transport cu homeostasis fungal pathogenesis pib-type atpase crp Infectious and parasitic diseases RC109-216
spellingShingle	copper (cu) transport cu homeostasis fungal pathogenesis pib-type atpase crp Infectious and parasitic diseases RC109-216 Damaris Lorenzo-Gutiérrez Lucía Gómez-Gil Josep Guarro M. Isabel G. Roncero Javier Capilla Loida López-Fernández Cu transporter protein CrpF protects against Cu-induced toxicity in Fusarium oxysporum
description	Cu is an essential trace element for cell growth and proliferation. However, excess of Cu accumulation leads to cellular toxicity. Thus, precise and tight regulation of Cu homeostasis processes, including transport, delivery, storage, detoxification, and efflux machineries, is required. Moreover, the maintenance of Cu homeostasis is critical for the survival and virulence of fungal pathogens. Cu homeostasis has been extensively studied in mammals, bacteria, and yeast, but it has not yet been well documented in filamentous fungi. In the present work, we investigated Cu tolerance in the filamentous fungus Fusarium oxysporum by analysing the Cu transporter coding gene crpF, previously studied in Aspergillus fumigatus. The expression studies demonstrated that crpF is upregulated in the presence of Cu and its deletion leads to severe sensitivity to low levels of CuSO4 in F. oxysporum. Targeted deletion of crpF did not significantly alter the resistance of the fungus to macrophage killing, nor its pathogenic behaviour on the tomato plants. However, the targeted deletion mutant ΔcrpF showed increased virulence in a murine model of systemic infection compared to wild-type strain (wt).
format	article
author	Damaris Lorenzo-Gutiérrez Lucía Gómez-Gil Josep Guarro M. Isabel G. Roncero Javier Capilla Loida López-Fernández
author_facet	Damaris Lorenzo-Gutiérrez Lucía Gómez-Gil Josep Guarro M. Isabel G. Roncero Javier Capilla Loida López-Fernández
author_sort	Damaris Lorenzo-Gutiérrez
title	Cu transporter protein CrpF protects against Cu-induced toxicity in Fusarium oxysporum
title_short	Cu transporter protein CrpF protects against Cu-induced toxicity in Fusarium oxysporum
title_full	Cu transporter protein CrpF protects against Cu-induced toxicity in Fusarium oxysporum
title_fullStr	Cu transporter protein CrpF protects against Cu-induced toxicity in Fusarium oxysporum
title_full_unstemmed	Cu transporter protein CrpF protects against Cu-induced toxicity in Fusarium oxysporum
title_sort	cu transporter protein crpf protects against cu-induced toxicity in fusarium oxysporum
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/2aa5659f50f14c189e867443002abfcf
work_keys_str_mv	AT damarislorenzogutierrez cutransporterproteincrpfprotectsagainstcuinducedtoxicityinfusariumoxysporum AT luciagomezgil cutransporterproteincrpfprotectsagainstcuinducedtoxicityinfusariumoxysporum AT josepguarro cutransporterproteincrpfprotectsagainstcuinducedtoxicityinfusariumoxysporum AT misabelgroncero cutransporterproteincrpfprotectsagainstcuinducedtoxicityinfusariumoxysporum AT javiercapilla cutransporterproteincrpfprotectsagainstcuinducedtoxicityinfusariumoxysporum AT loidalopezfernandez cutransporterproteincrpfprotectsagainstcuinducedtoxicityinfusariumoxysporum
_version_	1718425471013617664

Cu transporter protein CrpF protects against Cu-induced toxicity in Fusarium oxysporum

Ejemplares similares