Detection of icaAD Gene of Biofilm-Producing Staphylococcus aureus Carriage Isolates Obtained from Health Care Workers and Healthy Communities in Banyumas, Indonesia

Background: Asymptomatic biofilm-producing Staphylococcus aureus carriage play a pivotal role as a reservoir pathogen and increase the transmission rate in hospital as well as in healthy community. Biofilm- producing S. aureus which is regulated by the the ica AD gene reduce the antimicrobial abilit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Gembong Satria Mahardhika, Metta Ayu Susanti, Lantip Rujito, Dwi Utami Anjarwati
Formato:	article
Lenguaje:	EN
Publicado:	Diponegoro University 2020
Materias:	antibiotic resistance microtiter plate biofilm assay mrsa mssa nasal swab Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2aae6cc981b14019b137849dc4b3dd83
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2aae6cc981b14019b137849dc4b3dd83
record_format	dspace
spelling	oai:doaj.org-article:2aae6cc981b14019b137849dc4b3dd832021-11-05T16:47:42ZDetection of icaAD Gene of Biofilm-Producing Staphylococcus aureus Carriage Isolates Obtained from Health Care Workers and Healthy Communities in Banyumas, Indonesia2503-217810.14710/jbtr.v6i1.6135https://doaj.org/article/2aae6cc981b14019b137849dc4b3dd832020-04-01T00:00:00Zhttps://ejournal2.undip.ac.id/index.php/jbtr/article/view/6135https://doaj.org/toc/2503-2178Background: Asymptomatic biofilm-producing Staphylococcus aureus carriage play a pivotal role as a reservoir pathogen and increase the transmission rate in hospital as well as in healthy community. Biofilm- producing S. aureus which is regulated by the the ica AD gene reduce the antimicrobial ability in eliminating the pathogen. Objective: The aim of this study was to detect the icaAD gene of biofilm-producing Staphylococcus aureus carriage isolates obtained from healthcare workers and healthy Community in Banyumas, Indonesia. Methods: This descriptive observational study enrolled 60 healthcare workers and 60 healthy communities in Banyumas district. Antibiotic susceptibility test was using disc diffusion according to Clinical laboratory Standard Institute (CLSI) 2019. Biofilm-producing ability identified by using microtiter plate biofilm assay and the positivity of icaAD gene was performed by using PCR method. Results: The results showed that one of 60 healthcare workers (0,017%) showed MRSA, four of 60 healthcare workers (0,07%) were MSSA and 2 samples from community (0,03%) were MSSA. Total of 7 samples underwent biofilm examination, one sample was moderate biofilm, two samples were weak biofilm, and four samples were no biofilm. It was known that three biofilm-producing S.aureus were positive ica A/D gene. Conclusion: The ica A/D gene was found positive in both biofilm-producing MRSA and MSSA strain from both healthcare workers group and the healthy communities group. The presence of icaAD genes in both strains shows the potential for antibiotic resistance in these strains regulated by different mechanisms.Gembong Satria MahardhikaMetta Ayu SusantiLantip RujitoDwi Utami AnjarwatiDiponegoro Universityarticleantibiotic resistancemicrotiter plate biofilm assaymrsamssanasal swabMedicine (General)R5-920ENJournal of Biomedicine and Translational Research, Vol 6, Iss 1, Pp 15-18 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	antibiotic resistance microtiter plate biofilm assay mrsa mssa nasal swab Medicine (General) R5-920
spellingShingle	antibiotic resistance microtiter plate biofilm assay mrsa mssa nasal swab Medicine (General) R5-920 Gembong Satria Mahardhika Metta Ayu Susanti Lantip Rujito Dwi Utami Anjarwati Detection of icaAD Gene of Biofilm-Producing Staphylococcus aureus Carriage Isolates Obtained from Health Care Workers and Healthy Communities in Banyumas, Indonesia
description	Background: Asymptomatic biofilm-producing Staphylococcus aureus carriage play a pivotal role as a reservoir pathogen and increase the transmission rate in hospital as well as in healthy community. Biofilm- producing S. aureus which is regulated by the the ica AD gene reduce the antimicrobial ability in eliminating the pathogen. Objective: The aim of this study was to detect the icaAD gene of biofilm-producing Staphylococcus aureus carriage isolates obtained from healthcare workers and healthy Community in Banyumas, Indonesia. Methods: This descriptive observational study enrolled 60 healthcare workers and 60 healthy communities in Banyumas district. Antibiotic susceptibility test was using disc diffusion according to Clinical laboratory Standard Institute (CLSI) 2019. Biofilm-producing ability identified by using microtiter plate biofilm assay and the positivity of icaAD gene was performed by using PCR method. Results: The results showed that one of 60 healthcare workers (0,017%) showed MRSA, four of 60 healthcare workers (0,07%) were MSSA and 2 samples from community (0,03%) were MSSA. Total of 7 samples underwent biofilm examination, one sample was moderate biofilm, two samples were weak biofilm, and four samples were no biofilm. It was known that three biofilm-producing S.aureus were positive ica A/D gene. Conclusion: The ica A/D gene was found positive in both biofilm-producing MRSA and MSSA strain from both healthcare workers group and the healthy communities group. The presence of icaAD genes in both strains shows the potential for antibiotic resistance in these strains regulated by different mechanisms.
format	article
author	Gembong Satria Mahardhika Metta Ayu Susanti Lantip Rujito Dwi Utami Anjarwati
author_facet	Gembong Satria Mahardhika Metta Ayu Susanti Lantip Rujito Dwi Utami Anjarwati
author_sort	Gembong Satria Mahardhika
title	Detection of icaAD Gene of Biofilm-Producing Staphylococcus aureus Carriage Isolates Obtained from Health Care Workers and Healthy Communities in Banyumas, Indonesia
title_short	Detection of icaAD Gene of Biofilm-Producing Staphylococcus aureus Carriage Isolates Obtained from Health Care Workers and Healthy Communities in Banyumas, Indonesia
title_full	Detection of icaAD Gene of Biofilm-Producing Staphylococcus aureus Carriage Isolates Obtained from Health Care Workers and Healthy Communities in Banyumas, Indonesia
title_fullStr	Detection of icaAD Gene of Biofilm-Producing Staphylococcus aureus Carriage Isolates Obtained from Health Care Workers and Healthy Communities in Banyumas, Indonesia
title_full_unstemmed	Detection of icaAD Gene of Biofilm-Producing Staphylococcus aureus Carriage Isolates Obtained from Health Care Workers and Healthy Communities in Banyumas, Indonesia
title_sort	detection of icaad gene of biofilm-producing staphylococcus aureus carriage isolates obtained from health care workers and healthy communities in banyumas, indonesia
publisher	Diponegoro University
publishDate	2020
url	https://doaj.org/article/2aae6cc981b14019b137849dc4b3dd83
work_keys_str_mv	AT gembongsatriamahardhika detectionoficaadgeneofbiofilmproducingstaphylococcusaureuscarriageisolatesobtainedfromhealthcareworkersandhealthycommunitiesinbanyumasindonesia AT mettaayususanti detectionoficaadgeneofbiofilmproducingstaphylococcusaureuscarriageisolatesobtainedfromhealthcareworkersandhealthycommunitiesinbanyumasindonesia AT lantiprujito detectionoficaadgeneofbiofilmproducingstaphylococcusaureuscarriageisolatesobtainedfromhealthcareworkersandhealthycommunitiesinbanyumasindonesia AT dwiutamianjarwati detectionoficaadgeneofbiofilmproducingstaphylococcusaureuscarriageisolatesobtainedfromhealthcareworkersandhealthycommunitiesinbanyumasindonesia
_version_	1718444090805190656

Detection of icaAD Gene of Biofilm-Producing Staphylococcus aureus Carriage Isolates Obtained from Health Care Workers and Healthy Communities in Banyumas, Indonesia

Ejemplares similares