Effects of silver nanoparticles in combination with antibiotics on the resistant bacteria Acinetobacter baumannii

Guoqing Wan,1,2 Lingao Ruan,2,3 Yu Yin,2,3 Tian Yang,2,3 Mei Ge,2 Xiaodong Cheng1,4 1School of Life Science and Technology, China Pharmaceutical University, Nanjing, 2Shanghai Laiyi Center for Biopharmaceutical R&D, 3School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People&rsq...

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Autores principales:	Wan G, Ruan L, Yin Y, Yang T, Ge M, Cheng X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Acinetobacter baumannii AgNPs synergistic antibiotic combination antisense RNA Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2ab5b8282f714dbcad9eaaa863f74ae3
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spelling	oai:doaj.org-article:2ab5b8282f714dbcad9eaaa863f74ae32021-12-02T03:11:39ZEffects of silver nanoparticles in combination with antibiotics on the resistant bacteria Acinetobacter baumannii1178-2013https://doaj.org/article/2ab5b8282f714dbcad9eaaa863f74ae32016-08-01T00:00:00Zhttps://www.dovepress.com/effects-of-silver-nanoparticles--in-combination-with-antibiotics-on-th-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Guoqing Wan,1,2 Lingao Ruan,2,3 Yu Yin,2,3 Tian Yang,2,3 Mei Ge,2 Xiaodong Cheng1,4 1School of Life Science and Technology, China Pharmaceutical University, Nanjing, 2Shanghai Laiyi Center for Biopharmaceutical R&D, 3School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People’s Republic of China; 4Department of Integrative Biology & Pharmacology, The University of Texas Health Science Center, Houston, TX, USA Abstract: Acinetobacter baumannii resistance to carbapenem antibiotics is a serious clinical challenge. As a newly developed technology, silver nanoparticles (AgNPs) show some excellent characteristics compared to older treatments, and are a candidate for combating A. baumannii infection. However, its mechanism of action remains unclear. In this study, we combined AgNPs with antibiotics to treat carbapenem-resistant A. baumannii (aba1604). Our results showed that single AgNPs completely inhibited A. baumannii growth at 2.5 µg/mL. AgNP treatment also showed synergistic effects with the antibiotics polymixin B and rifampicin, and an additive effect with tigecyline. In vivo, we found that AgNPs–antibiotic combinations led to better survival ratios in A. baumannii-infected mouse peritonitis models than that by single drug treatment. Finally, we employed different antisense RNA-targeted Escherichia coli strains to elucidate the synergistic mechanism involved in bacterial responses to AgNPs and antibiotics. Keywords: Acinetobacter baumannii, AgNPs, synergistic, antibiotic combination, antisense RNA Wan GRuan LYin YYang TGe MCheng XDove Medical PressarticleAcinetobacter baumanniiAgNPssynergisticantibiotic combinationantisense RNAMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 11, Pp 3789-3800 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Acinetobacter baumannii AgNPs synergistic antibiotic combination antisense RNA Medicine (General) R5-920
spellingShingle	Acinetobacter baumannii AgNPs synergistic antibiotic combination antisense RNA Medicine (General) R5-920 Wan G Ruan L Yin Y Yang T Ge M Cheng X Effects of silver nanoparticles in combination with antibiotics on the resistant bacteria Acinetobacter baumannii
description	Guoqing Wan,1,2 Lingao Ruan,2,3 Yu Yin,2,3 Tian Yang,2,3 Mei Ge,2 Xiaodong Cheng1,4 1School of Life Science and Technology, China Pharmaceutical University, Nanjing, 2Shanghai Laiyi Center for Biopharmaceutical R&D, 3School of Pharmacy, Shanghai Jiao Tong University, Shanghai, People’s Republic of China; 4Department of Integrative Biology & Pharmacology, The University of Texas Health Science Center, Houston, TX, USA Abstract: Acinetobacter baumannii resistance to carbapenem antibiotics is a serious clinical challenge. As a newly developed technology, silver nanoparticles (AgNPs) show some excellent characteristics compared to older treatments, and are a candidate for combating A. baumannii infection. However, its mechanism of action remains unclear. In this study, we combined AgNPs with antibiotics to treat carbapenem-resistant A. baumannii (aba1604). Our results showed that single AgNPs completely inhibited A. baumannii growth at 2.5 µg/mL. AgNP treatment also showed synergistic effects with the antibiotics polymixin B and rifampicin, and an additive effect with tigecyline. In vivo, we found that AgNPs–antibiotic combinations led to better survival ratios in A. baumannii-infected mouse peritonitis models than that by single drug treatment. Finally, we employed different antisense RNA-targeted Escherichia coli strains to elucidate the synergistic mechanism involved in bacterial responses to AgNPs and antibiotics. Keywords: Acinetobacter baumannii, AgNPs, synergistic, antibiotic combination, antisense RNA
format	article
author	Wan G Ruan L Yin Y Yang T Ge M Cheng X
author_facet	Wan G Ruan L Yin Y Yang T Ge M Cheng X
author_sort	Wan G
title	Effects of silver nanoparticles in combination with antibiotics on the resistant bacteria Acinetobacter baumannii
title_short	Effects of silver nanoparticles in combination with antibiotics on the resistant bacteria Acinetobacter baumannii
title_full	Effects of silver nanoparticles in combination with antibiotics on the resistant bacteria Acinetobacter baumannii
title_fullStr	Effects of silver nanoparticles in combination with antibiotics on the resistant bacteria Acinetobacter baumannii
title_full_unstemmed	Effects of silver nanoparticles in combination with antibiotics on the resistant bacteria Acinetobacter baumannii
title_sort	effects of silver nanoparticles in combination with antibiotics on the resistant bacteria acinetobacter baumannii
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/2ab5b8282f714dbcad9eaaa863f74ae3
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Effects of silver nanoparticles in combination with antibiotics on the resistant bacteria Acinetobacter baumannii

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