Identification and Structure Prediction of Human Septin-4 as a Biomarker for Diagnosis of Asthenozoospermic Infertile Patients—Critical Finding Toward Personalized Medicine

Semen parameters are been found as a key factor to evaluate the count and morphology in the given semen sample. The deep knowledge of male infertility will unravel with semen parameters correlated with molecular and biochemical parameters. The current research study is to identify the motility assoc...

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Autores principales:	A. S. Vickram, K. Anbarasu, Palanivelu Jeyanthi, G. Gulothungan, R. Nanmaran, S. Thanigaivel, T. B. Sridharan, Karunakaran Rohini
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	human semen seminal plasma motility associated protein septin-4 in-silico characterization molecular dynamics simulations Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2abac572cab24a1fb55296e24ec8e03e
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spelling	oai:doaj.org-article:2abac572cab24a1fb55296e24ec8e03e2021-12-03T04:49:32ZIdentification and Structure Prediction of Human Septin-4 as a Biomarker for Diagnosis of Asthenozoospermic Infertile Patients—Critical Finding Toward Personalized Medicine2296-858X10.3389/fmed.2021.723019https://doaj.org/article/2abac572cab24a1fb55296e24ec8e03e2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmed.2021.723019/fullhttps://doaj.org/toc/2296-858XSemen parameters are been found as a key factor to evaluate the count and morphology in the given semen sample. The deep knowledge of male infertility will unravel with semen parameters correlated with molecular and biochemical parameters. The current research study is to identify the motility associated protein and its structure through the in-silico approach. Semen samples were collected and initial analysis including semen parameters was analyzed by using the World Health Organization protocol. Semen biochemical parameters, namely, seminal plasma protein concentration, fructose content, and glucosidase content were calculated and evaluated for correlation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) were carried out for identification of Septin-4 presence in the semen sample. Mascot search was done for protein conformation and in-silico characterization of Septin-4 by structural modeling in Iterative Threading Assembly Refinement (I-TASSER). Twenty-five nanoseconds molecular dynamics (MD) simulations results showed the stable nature of Septin-4 in the dynamic system. Overall, our results showed the presence of motility-associated protein in normospermia and control samples and not in the case of asthenospermia and oligoasthenospermia. Molecular techniques characterized the presence of Septin-4 and as a novel biomarker for infertility diagnosis.A. S. VickramK. AnbarasuPalanivelu JeyanthiG. GulothunganR. NanmaranS. ThanigaivelT. B. SridharanKarunakaran RohiniFrontiers Media S.A.articlehuman semenseminal plasmamotility associated proteinseptin-4in-silico characterizationmolecular dynamics simulationsMedicine (General)R5-920ENFrontiers in Medicine, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	human semen seminal plasma motility associated protein septin-4 in-silico characterization molecular dynamics simulations Medicine (General) R5-920
spellingShingle	human semen seminal plasma motility associated protein septin-4 in-silico characterization molecular dynamics simulations Medicine (General) R5-920 A. S. Vickram K. Anbarasu Palanivelu Jeyanthi G. Gulothungan R. Nanmaran S. Thanigaivel T. B. Sridharan Karunakaran Rohini Identification and Structure Prediction of Human Septin-4 as a Biomarker for Diagnosis of Asthenozoospermic Infertile Patients—Critical Finding Toward Personalized Medicine
description	Semen parameters are been found as a key factor to evaluate the count and morphology in the given semen sample. The deep knowledge of male infertility will unravel with semen parameters correlated with molecular and biochemical parameters. The current research study is to identify the motility associated protein and its structure through the in-silico approach. Semen samples were collected and initial analysis including semen parameters was analyzed by using the World Health Organization protocol. Semen biochemical parameters, namely, seminal plasma protein concentration, fructose content, and glucosidase content were calculated and evaluated for correlation. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) were carried out for identification of Septin-4 presence in the semen sample. Mascot search was done for protein conformation and in-silico characterization of Septin-4 by structural modeling in Iterative Threading Assembly Refinement (I-TASSER). Twenty-five nanoseconds molecular dynamics (MD) simulations results showed the stable nature of Septin-4 in the dynamic system. Overall, our results showed the presence of motility-associated protein in normospermia and control samples and not in the case of asthenospermia and oligoasthenospermia. Molecular techniques characterized the presence of Septin-4 and as a novel biomarker for infertility diagnosis.
format	article
author	A. S. Vickram K. Anbarasu Palanivelu Jeyanthi G. Gulothungan R. Nanmaran S. Thanigaivel T. B. Sridharan Karunakaran Rohini
author_facet	A. S. Vickram K. Anbarasu Palanivelu Jeyanthi G. Gulothungan R. Nanmaran S. Thanigaivel T. B. Sridharan Karunakaran Rohini
author_sort	A. S. Vickram
title	Identification and Structure Prediction of Human Septin-4 as a Biomarker for Diagnosis of Asthenozoospermic Infertile Patients—Critical Finding Toward Personalized Medicine
title_short	Identification and Structure Prediction of Human Septin-4 as a Biomarker for Diagnosis of Asthenozoospermic Infertile Patients—Critical Finding Toward Personalized Medicine
title_full	Identification and Structure Prediction of Human Septin-4 as a Biomarker for Diagnosis of Asthenozoospermic Infertile Patients—Critical Finding Toward Personalized Medicine
title_fullStr	Identification and Structure Prediction of Human Septin-4 as a Biomarker for Diagnosis of Asthenozoospermic Infertile Patients—Critical Finding Toward Personalized Medicine
title_full_unstemmed	Identification and Structure Prediction of Human Septin-4 as a Biomarker for Diagnosis of Asthenozoospermic Infertile Patients—Critical Finding Toward Personalized Medicine
title_sort	identification and structure prediction of human septin-4 as a biomarker for diagnosis of asthenozoospermic infertile patients—critical finding toward personalized medicine
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/2abac572cab24a1fb55296e24ec8e03e
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Identification and Structure Prediction of Human Septin-4 as a Biomarker for Diagnosis of Asthenozoospermic Infertile Patients—Critical Finding Toward Personalized Medicine

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