Characterization of zebrafish GABAA receptor subunits

Abstract γ-Aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system, exerts its effect through the activation of GABA receptors. GABAA receptors are ligand-gated chloride channels composed of five subunit proteins. Mammals have 19 different GABAA receptor subunit...

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Autores principales:	Kenichiro Sadamitsu, Leona Shigemitsu, Marina Suzuki, Daishi Ito, Makoto Kashima, Hiromi Hirata
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2abbb96b7661477e829bc4385ad3d9e4
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spelling	oai:doaj.org-article:2abbb96b7661477e829bc4385ad3d9e42021-12-02T17:05:46ZCharacterization of zebrafish GABAA receptor subunits10.1038/s41598-021-84646-32045-2322https://doaj.org/article/2abbb96b7661477e829bc4385ad3d9e42021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84646-3https://doaj.org/toc/2045-2322Abstract γ-Aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system, exerts its effect through the activation of GABA receptors. GABAA receptors are ligand-gated chloride channels composed of five subunit proteins. Mammals have 19 different GABAA receptor subunits (α1–6, β1–3, γ1–3, δ, ε, π, θ, and ρ1–3), the physiological properties of which have been assayed by electrophysiology. However, the evolutionary conservation of the physiological characteristics of diverged GABAA receptor subunits remains unclear. Zebrafish have 23 subunits (α1, α2a, α2b, α3–5, α6a, α6b, β1–4, γ1–3, δ, π, ζ, ρ1, ρ2a, ρ2b, ρ3a, and ρ3b), but the electrophysiological properties of these subunits have not been explored. In this study, we cloned the coding sequences for zebrafish GABAA receptor subunits and investigated their expression patterns in larval zebrafish by whole-mount in situ hybridization. We also performed electrophysiological recordings of GABA-evoked currents from Xenopus oocytes injected with one or multiple zebrafish GABAA receptor subunit cRNAs and calculated the half-maximal effective concentrations (EC50s) for each. Our results revealed the spatial expressions and electrophysiological GABA sensitivities of zebrafish GABAA receptors, suggesting that the properties of GABAA receptor subunits are conserved among vertebrates.Kenichiro SadamitsuLeona ShigemitsuMarina SuzukiDaishi ItoMakoto KashimaHiromi HirataNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kenichiro Sadamitsu Leona Shigemitsu Marina Suzuki Daishi Ito Makoto Kashima Hiromi Hirata Characterization of zebrafish GABAA receptor subunits
description	Abstract γ-Aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system, exerts its effect through the activation of GABA receptors. GABAA receptors are ligand-gated chloride channels composed of five subunit proteins. Mammals have 19 different GABAA receptor subunits (α1–6, β1–3, γ1–3, δ, ε, π, θ, and ρ1–3), the physiological properties of which have been assayed by electrophysiology. However, the evolutionary conservation of the physiological characteristics of diverged GABAA receptor subunits remains unclear. Zebrafish have 23 subunits (α1, α2a, α2b, α3–5, α6a, α6b, β1–4, γ1–3, δ, π, ζ, ρ1, ρ2a, ρ2b, ρ3a, and ρ3b), but the electrophysiological properties of these subunits have not been explored. In this study, we cloned the coding sequences for zebrafish GABAA receptor subunits and investigated their expression patterns in larval zebrafish by whole-mount in situ hybridization. We also performed electrophysiological recordings of GABA-evoked currents from Xenopus oocytes injected with one or multiple zebrafish GABAA receptor subunit cRNAs and calculated the half-maximal effective concentrations (EC50s) for each. Our results revealed the spatial expressions and electrophysiological GABA sensitivities of zebrafish GABAA receptors, suggesting that the properties of GABAA receptor subunits are conserved among vertebrates.
format	article
author	Kenichiro Sadamitsu Leona Shigemitsu Marina Suzuki Daishi Ito Makoto Kashima Hiromi Hirata
author_facet	Kenichiro Sadamitsu Leona Shigemitsu Marina Suzuki Daishi Ito Makoto Kashima Hiromi Hirata
author_sort	Kenichiro Sadamitsu
title	Characterization of zebrafish GABAA receptor subunits
title_short	Characterization of zebrafish GABAA receptor subunits
title_full	Characterization of zebrafish GABAA receptor subunits
title_fullStr	Characterization of zebrafish GABAA receptor subunits
title_full_unstemmed	Characterization of zebrafish GABAA receptor subunits
title_sort	characterization of zebrafish gabaa receptor subunits
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2abbb96b7661477e829bc4385ad3d9e4
work_keys_str_mv	AT kenichirosadamitsu characterizationofzebrafishgabaareceptorsubunits AT leonashigemitsu characterizationofzebrafishgabaareceptorsubunits AT marinasuzuki characterizationofzebrafishgabaareceptorsubunits AT daishiito characterizationofzebrafishgabaareceptorsubunits AT makotokashima characterizationofzebrafishgabaareceptorsubunits AT hiromihirata characterizationofzebrafishgabaareceptorsubunits
_version_	1718381780861452288

Characterization of zebrafish GABAA receptor subunits

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