FPGA Integrated Optofluidic Biosensor for Real-Time Single Biomarker Analysis

Integrated optofluidic biosensors can fill the need for sensitive, amplification-free, multiplex single molecule detection which is relevant for containing the spread of infectious diseases such as COVID-19. Here, we demonstrate a rapid sample-to-answer scheme that uses a field programmable gate arr...

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Autores principales:	Mohammad Julker Neyen Sampad, Md Nafiz Amin, Aaron R. Hawkins, Holger Schmidt
Formato:	article
Lenguaje:	EN
Publicado:	IEEE 2022
Materias:	Optofluidics single molecule detection biophotonics field programmable gate array (FPGA) anti-resonant reflecting optical waveguide (ARROW) real-time Applied optics. Photonics TA1501-1820 Optics. Light QC350-467
Acceso en línea:	https://doaj.org/article/2abe3a68bf7e425d9536d852299be2b1
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id	oai:doaj.org-article:2abe3a68bf7e425d9536d852299be2b1
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spelling	oai:doaj.org-article:2abe3a68bf7e425d9536d852299be2b12021-11-30T00:00:10ZFPGA Integrated Optofluidic Biosensor for Real-Time Single Biomarker Analysis1943-065510.1109/JPHOT.2021.3127484https://doaj.org/article/2abe3a68bf7e425d9536d852299be2b12022-01-01T00:00:00Zhttps://ieeexplore.ieee.org/document/9612033/https://doaj.org/toc/1943-0655Integrated optofluidic biosensors can fill the need for sensitive, amplification-free, multiplex single molecule detection which is relevant for containing the spread of infectious diseases such as COVID-19. Here, we demonstrate a rapid sample-to-answer scheme that uses a field programmable gate array (FPGA) to enable live monitoring of single particle fluorescence analysis on an optofluidic chip. Fluorescent nanobeads flowing through a micro channel are detected with 99% accuracy and particle concentrations in clinically relevant ranges from 3.4x10<sup>4</sup> to 3.4 × 10<sup>6</sup>/ml are determined within seconds to a few minutes without the need for post-experiment data extraction and analysis. In addition, other extract salient experimental parameters such as dynamic flow rate changes can be monitored in real time. The sensor is validated with real-time fluorescence detection of single bacterial plasmid DNA at attomolar concentrations, showing excellent promise for implementation as a point of care (POC) diagnostic tool.Mohammad Julker Neyen SampadMd Nafiz AminAaron R. HawkinsHolger SchmidtIEEEarticleOptofluidicssingle molecule detectionbiophotonicsfield programmable gate array (FPGA)anti-resonant reflecting optical waveguide (ARROW)real-timeApplied optics. PhotonicsTA1501-1820Optics. LightQC350-467ENIEEE Photonics Journal, Vol 14, Iss 1, Pp 1-6 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Optofluidics single molecule detection biophotonics field programmable gate array (FPGA) anti-resonant reflecting optical waveguide (ARROW) real-time Applied optics. Photonics TA1501-1820 Optics. Light QC350-467
spellingShingle	Optofluidics single molecule detection biophotonics field programmable gate array (FPGA) anti-resonant reflecting optical waveguide (ARROW) real-time Applied optics. Photonics TA1501-1820 Optics. Light QC350-467 Mohammad Julker Neyen Sampad Md Nafiz Amin Aaron R. Hawkins Holger Schmidt FPGA Integrated Optofluidic Biosensor for Real-Time Single Biomarker Analysis
description	Integrated optofluidic biosensors can fill the need for sensitive, amplification-free, multiplex single molecule detection which is relevant for containing the spread of infectious diseases such as COVID-19. Here, we demonstrate a rapid sample-to-answer scheme that uses a field programmable gate array (FPGA) to enable live monitoring of single particle fluorescence analysis on an optofluidic chip. Fluorescent nanobeads flowing through a micro channel are detected with 99% accuracy and particle concentrations in clinically relevant ranges from 3.4x10<sup>4</sup> to 3.4 × 10<sup>6</sup>/ml are determined within seconds to a few minutes without the need for post-experiment data extraction and analysis. In addition, other extract salient experimental parameters such as dynamic flow rate changes can be monitored in real time. The sensor is validated with real-time fluorescence detection of single bacterial plasmid DNA at attomolar concentrations, showing excellent promise for implementation as a point of care (POC) diagnostic tool.
format	article
author	Mohammad Julker Neyen Sampad Md Nafiz Amin Aaron R. Hawkins Holger Schmidt
author_facet	Mohammad Julker Neyen Sampad Md Nafiz Amin Aaron R. Hawkins Holger Schmidt
author_sort	Mohammad Julker Neyen Sampad
title	FPGA Integrated Optofluidic Biosensor for Real-Time Single Biomarker Analysis
title_short	FPGA Integrated Optofluidic Biosensor for Real-Time Single Biomarker Analysis
title_full	FPGA Integrated Optofluidic Biosensor for Real-Time Single Biomarker Analysis
title_fullStr	FPGA Integrated Optofluidic Biosensor for Real-Time Single Biomarker Analysis
title_full_unstemmed	FPGA Integrated Optofluidic Biosensor for Real-Time Single Biomarker Analysis
title_sort	fpga integrated optofluidic biosensor for real-time single biomarker analysis
publisher	IEEE
publishDate	2022
url	https://doaj.org/article/2abe3a68bf7e425d9536d852299be2b1
work_keys_str_mv	AT mohammadjulkerneyensampad fpgaintegratedoptofluidicbiosensorforrealtimesinglebiomarkeranalysis AT mdnafizamin fpgaintegratedoptofluidicbiosensorforrealtimesinglebiomarkeranalysis AT aaronrhawkins fpgaintegratedoptofluidicbiosensorforrealtimesinglebiomarkeranalysis AT holgerschmidt fpgaintegratedoptofluidicbiosensorforrealtimesinglebiomarkeranalysis
_version_	1718406852578902016

FPGA Integrated Optofluidic Biosensor for Real-Time Single Biomarker Analysis

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