Analysis and differentiation of seminal plasma via polarized SERS spectroscopy

Xiwen Chen,1,* Zufang Huang,1,* Shangyuan Feng,1 Jinhua Chen,2 Lan Wang,1 Peng Lu,1 Haishan Zeng,3 Rong Chen1 1Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou,...

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Autores principales:	Chen X, Huang Z, Feng S, Chen J, Wang L, Lu P, Zeng H, Chen R
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2ac2e517b6514103aab0b469e41da0da
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spelling	oai:doaj.org-article:2ac2e517b6514103aab0b469e41da0da2021-12-02T02:56:19ZAnalysis and differentiation of seminal plasma via polarized SERS spectroscopy1176-91141178-2013https://doaj.org/article/2ac2e517b6514103aab0b469e41da0da2012-12-01T00:00:00Zhttp://www.dovepress.com/analysis-and-differentiation-of-seminal-plasma-via-polarized-sers-spec-a11767https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Xiwen Chen,1,* Zufang Huang,1,* Shangyuan Feng,1 Jinhua Chen,2 Lan Wang,1 Peng Lu,1 Haishan Zeng,3 Rong Chen1 1Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, China; 2Fujian Provincial Hospital, Fuzhou, China; 3Imaging Unit &ndash; Integrative Oncology Department, British Columbia Cancer Agency Research Centre, Vancouver, BC, Canada*These authors contributed equally to this workAbstract: Polarized surface-enhanced Raman scattering (SERS) spectroscopy was applied for obtaining biochemical information about the seminal plasma. The effect of different laser polarizations (nonpolarized, linear-polarized, right-handed circularly polarized, and left-handed circularly polarized) on seminal plasma SERS spectroscopy was explored for the first time. The diagnostic performance in differentiating abnormal seminal plasma (n = 37) from normal seminal plasma (n = 24) was evaluated. A combination of principal component analysis (PCA) and linear discriminant analysis (LDA) was employed to develop diagnostic algorithms. Classification results of different laser polarizations demonstrated different diagnostic sensitivities and specificities, among which, left-handed circularly polarized laser excitation showed the best diagnostic result (95.8% sensitivity and 64.9% specificity). Our exploratory study demonstrated that SERS spectroscopy with left-handed circularly polarized laser excitation has the potential for becoming a new diagnostic method in semen-quality assessment.Keywords: SERS, seminal plasma, PCA-LDA, polarized laser excitation, silver nanoparticles, biomolecule chiralityChen XHuang ZFeng SChen JWang LLu PZeng HChen RDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 6115-6121 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chen X Huang Z Feng S Chen J Wang L Lu P Zeng H Chen R Analysis and differentiation of seminal plasma via polarized SERS spectroscopy
description	Xiwen Chen,1,* Zufang Huang,1,* Shangyuan Feng,1 Jinhua Chen,2 Lan Wang,1 Peng Lu,1 Haishan Zeng,3 Rong Chen1 1Key Laboratory of Opto-Electronic Science and Technology for Medicine of Ministry of Education, Fujian Provincial Key Laboratory for Photonics Technology, Fujian Normal University, Fuzhou, China; 2Fujian Provincial Hospital, Fuzhou, China; 3Imaging Unit &ndash; Integrative Oncology Department, British Columbia Cancer Agency Research Centre, Vancouver, BC, Canada*These authors contributed equally to this workAbstract: Polarized surface-enhanced Raman scattering (SERS) spectroscopy was applied for obtaining biochemical information about the seminal plasma. The effect of different laser polarizations (nonpolarized, linear-polarized, right-handed circularly polarized, and left-handed circularly polarized) on seminal plasma SERS spectroscopy was explored for the first time. The diagnostic performance in differentiating abnormal seminal plasma (n = 37) from normal seminal plasma (n = 24) was evaluated. A combination of principal component analysis (PCA) and linear discriminant analysis (LDA) was employed to develop diagnostic algorithms. Classification results of different laser polarizations demonstrated different diagnostic sensitivities and specificities, among which, left-handed circularly polarized laser excitation showed the best diagnostic result (95.8% sensitivity and 64.9% specificity). Our exploratory study demonstrated that SERS spectroscopy with left-handed circularly polarized laser excitation has the potential for becoming a new diagnostic method in semen-quality assessment.Keywords: SERS, seminal plasma, PCA-LDA, polarized laser excitation, silver nanoparticles, biomolecule chirality
format	article
author	Chen X Huang Z Feng S Chen J Wang L Lu P Zeng H Chen R
author_facet	Chen X Huang Z Feng S Chen J Wang L Lu P Zeng H Chen R
author_sort	Chen X
title	Analysis and differentiation of seminal plasma via polarized SERS spectroscopy
title_short	Analysis and differentiation of seminal plasma via polarized SERS spectroscopy
title_full	Analysis and differentiation of seminal plasma via polarized SERS spectroscopy
title_fullStr	Analysis and differentiation of seminal plasma via polarized SERS spectroscopy
title_full_unstemmed	Analysis and differentiation of seminal plasma via polarized SERS spectroscopy
title_sort	analysis and differentiation of seminal plasma via polarized sers spectroscopy
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/2ac2e517b6514103aab0b469e41da0da
work_keys_str_mv	AT chenx analysisanddifferentiationofseminalplasmaviapolarizedsersspectroscopy AT huangz analysisanddifferentiationofseminalplasmaviapolarizedsersspectroscopy AT fengs analysisanddifferentiationofseminalplasmaviapolarizedsersspectroscopy AT chenj analysisanddifferentiationofseminalplasmaviapolarizedsersspectroscopy AT wangl analysisanddifferentiationofseminalplasmaviapolarizedsersspectroscopy AT lup analysisanddifferentiationofseminalplasmaviapolarizedsersspectroscopy AT zengh analysisanddifferentiationofseminalplasmaviapolarizedsersspectroscopy AT chenr analysisanddifferentiationofseminalplasmaviapolarizedsersspectroscopy
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Analysis and differentiation of seminal plasma via polarized SERS spectroscopy

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