Translating Lung Microbiome Profiles into the Next-Generation Diagnostic Gold Standard for Pneumonia: a Clinical Investigator’s Perspective

ABSTRACT Severe bacterial pneumonia is a major global cause of morbidity and mortality, yet current diagnostic approaches rely on identification of causative pathogens by cultures, which require extended incubation periods and often fail to detect relevant pathogens. Consequently, patients are presc...

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Autor principal:	Georgios D. Kitsios
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	intensive care unit lung microbiome metagenomics next-generation sequencing pneumonia Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2aca53aa734848e1bb258b471c0beb1b
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spelling	oai:doaj.org-article:2aca53aa734848e1bb258b471c0beb1b2021-12-02T18:15:47ZTranslating Lung Microbiome Profiles into the Next-Generation Diagnostic Gold Standard for Pneumonia: a Clinical Investigator’s Perspective10.1128/mSystems.00153-172379-5077https://doaj.org/article/2aca53aa734848e1bb258b471c0beb1b2018-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00153-17https://doaj.org/toc/2379-5077ABSTRACT Severe bacterial pneumonia is a major global cause of morbidity and mortality, yet current diagnostic approaches rely on identification of causative pathogens by cultures, which require extended incubation periods and often fail to detect relevant pathogens. Consequently, patients are prescribed broad-spectrum antibiotics in a “one-size-fits-all” manner, which may be inappropriate for their individual needs and promote antibiotic resistance. My research focuses on leveraging next-generation sequencing of microbial DNA directly from patient samples for the development of new, culture-independent definitions of pneumonia. In this perspective article, I discuss the current state of the field and focus on the conceptual and research design challenges for clinical translation. With ongoing technological advancements and application of computational biology methods for assessing clinical validity and utility, I anticipate that sequencing-based diagnostics will soon be able to positively disrupt the way we think about, diagnose, and treat pulmonary infections.Georgios D. KitsiosAmerican Society for Microbiologyarticleintensive care unitlung microbiomemetagenomicsnext-generation sequencingpneumoniaMicrobiologyQR1-502ENmSystems, Vol 3, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	intensive care unit lung microbiome metagenomics next-generation sequencing pneumonia Microbiology QR1-502
spellingShingle	intensive care unit lung microbiome metagenomics next-generation sequencing pneumonia Microbiology QR1-502 Georgios D. Kitsios Translating Lung Microbiome Profiles into the Next-Generation Diagnostic Gold Standard for Pneumonia: a Clinical Investigator’s Perspective
description	ABSTRACT Severe bacterial pneumonia is a major global cause of morbidity and mortality, yet current diagnostic approaches rely on identification of causative pathogens by cultures, which require extended incubation periods and often fail to detect relevant pathogens. Consequently, patients are prescribed broad-spectrum antibiotics in a “one-size-fits-all” manner, which may be inappropriate for their individual needs and promote antibiotic resistance. My research focuses on leveraging next-generation sequencing of microbial DNA directly from patient samples for the development of new, culture-independent definitions of pneumonia. In this perspective article, I discuss the current state of the field and focus on the conceptual and research design challenges for clinical translation. With ongoing technological advancements and application of computational biology methods for assessing clinical validity and utility, I anticipate that sequencing-based diagnostics will soon be able to positively disrupt the way we think about, diagnose, and treat pulmonary infections.
format	article
author	Georgios D. Kitsios
author_facet	Georgios D. Kitsios
author_sort	Georgios D. Kitsios
title	Translating Lung Microbiome Profiles into the Next-Generation Diagnostic Gold Standard for Pneumonia: a Clinical Investigator’s Perspective
title_short	Translating Lung Microbiome Profiles into the Next-Generation Diagnostic Gold Standard for Pneumonia: a Clinical Investigator’s Perspective
title_full	Translating Lung Microbiome Profiles into the Next-Generation Diagnostic Gold Standard for Pneumonia: a Clinical Investigator’s Perspective
title_fullStr	Translating Lung Microbiome Profiles into the Next-Generation Diagnostic Gold Standard for Pneumonia: a Clinical Investigator’s Perspective
title_full_unstemmed	Translating Lung Microbiome Profiles into the Next-Generation Diagnostic Gold Standard for Pneumonia: a Clinical Investigator’s Perspective
title_sort	translating lung microbiome profiles into the next-generation diagnostic gold standard for pneumonia: a clinical investigator’s perspective
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/2aca53aa734848e1bb258b471c0beb1b
work_keys_str_mv	AT georgiosdkitsios translatinglungmicrobiomeprofilesintothenextgenerationdiagnosticgoldstandardforpneumoniaaclinicalinvestigatorsperspective
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Translating Lung Microbiome Profiles into the Next-Generation Diagnostic Gold Standard for Pneumonia: a Clinical Investigator’s Perspective

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