The Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate)

INTRODUCTION: ROS is the most common cause of male infertility. Monosodium glutamate (MSG) is a compound which produces ROS and decreases FSH and LH levels. Continuous Multistage Countercurrent Extraction (CMCE) propolis extract as an antioxidant is evidently capable of reducing ROS concentration....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rahmawati Rahmawati, Taufiqurrachman Nasihun, Israhnanto Isradji
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Sultan Agung Semarang 2020
Materias:	msg, cmce propolis extract, leydig cells count, sertoli cells and sperm quality Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2acac12a5c3745acb376b2d1b813828d
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2acac12a5c3745acb376b2d1b813828d
record_format	dspace
spelling	oai:doaj.org-article:2acac12a5c3745acb376b2d1b813828d2021-11-25T13:16:27ZThe Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate)2085-15452339-093X10.30659/sainsmed.v10i2.4221https://doaj.org/article/2acac12a5c3745acb376b2d1b813828d2020-01-01T00:00:00Zhttp://jurnal.unissula.ac.id/index.php/sainsmedika/article/view/4221https://doaj.org/toc/2085-1545https://doaj.org/toc/2339-093XINTRODUCTION: ROS is the most common cause of male infertility. Monosodium glutamate (MSG) is a compound which produces ROS and decreases FSH and LH levels. Continuous Multistage Countercurrent Extraction (CMCE) propolis extract as an antioxidant is evidently capable of reducing ROS concentration. OBJECTIVE: this research aims at evaluating the effect of CMCE propolis extract administration on Leydig cells and Sertoli cells counts and sperm quality of MSG-induced male wistar rats. METHODS: employing the Post Test Only Control Group Design, 18 rats were divided into 3 groups: control group, treated only with MSG (MS-G), and treatment groups, respectively treated with CMCE propolis extract at doses 8.3mg/day (CM8-G) and 10.8mg/day (CM10-G). CMCE propolis extract was given orally for 21 days. At the end of treatment, sperm and testis were taken to evaluate the Leydig cells and Sertoli cells counts and sperm quality. The sperm obtained from epididymis was analyzed using the WHO standards, while Leydig cells and Sertoli cells were counted from preparation using the HE stain method. RESULTS: the Post Hoc analysis result shows that the Leydig and Sertoli cells counts and sperm quality of CM8-G and CM10-G are significantly higher than those of MS-G, p<0.05. The Leydig cells and Sertoli cells counts and sperm quality of CM10-G are significantly higher than those of CM8-G, p><0.05. CONCLUSION: CMCE propolis extract administration evidently increases the Leydig cells and Sertoli cells counts and sperm quality from the perspective of the count, motility and morphology of spermatozoa of MSG-induced male wistar rats.><0.05 CONCLUSION: CMCE propolis extract administration evidently increases the Leydig cells and Sertoli cells counts and sperm quality from the perspective of the count, motility and morphology of spermatozoa of MSG-induced male wistar rats.Rahmawati RahmawatiTaufiqurrachman NasihunIsrahnanto IsradjiUniversitas Sultan Agung Semarangarticlemsg, cmce propolis extract, leydig cells count, sertoli cells and sperm qualityMedicine (General)R5-920ENSains Medika, Vol 10, Iss 2, Pp 62-67 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	msg, cmce propolis extract, leydig cells count, sertoli cells and sperm quality Medicine (General) R5-920
spellingShingle	msg, cmce propolis extract, leydig cells count, sertoli cells and sperm quality Medicine (General) R5-920 Rahmawati Rahmawati Taufiqurrachman Nasihun Israhnanto Isradji The Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate)
description	INTRODUCTION: ROS is the most common cause of male infertility. Monosodium glutamate (MSG) is a compound which produces ROS and decreases FSH and LH levels. Continuous Multistage Countercurrent Extraction (CMCE) propolis extract as an antioxidant is evidently capable of reducing ROS concentration. OBJECTIVE: this research aims at evaluating the effect of CMCE propolis extract administration on Leydig cells and Sertoli cells counts and sperm quality of MSG-induced male wistar rats. METHODS: employing the Post Test Only Control Group Design, 18 rats were divided into 3 groups: control group, treated only with MSG (MS-G), and treatment groups, respectively treated with CMCE propolis extract at doses 8.3mg/day (CM8-G) and 10.8mg/day (CM10-G). CMCE propolis extract was given orally for 21 days. At the end of treatment, sperm and testis were taken to evaluate the Leydig cells and Sertoli cells counts and sperm quality. The sperm obtained from epididymis was analyzed using the WHO standards, while Leydig cells and Sertoli cells were counted from preparation using the HE stain method. RESULTS: the Post Hoc analysis result shows that the Leydig and Sertoli cells counts and sperm quality of CM8-G and CM10-G are significantly higher than those of MS-G, p<0.05. The Leydig cells and Sertoli cells counts and sperm quality of CM10-G are significantly higher than those of CM8-G, p><0.05. CONCLUSION: CMCE propolis extract administration evidently increases the Leydig cells and Sertoli cells counts and sperm quality from the perspective of the count, motility and morphology of spermatozoa of MSG-induced male wistar rats.><0.05 CONCLUSION: CMCE propolis extract administration evidently increases the Leydig cells and Sertoli cells counts and sperm quality from the perspective of the count, motility and morphology of spermatozoa of MSG-induced male wistar rats.
format	article
author	Rahmawati Rahmawati Taufiqurrachman Nasihun Israhnanto Isradji
author_facet	Rahmawati Rahmawati Taufiqurrachman Nasihun Israhnanto Isradji
author_sort	Rahmawati Rahmawati
title	The Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate)
title_short	The Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate)
title_full	The Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate)
title_fullStr	The Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate)
title_full_unstemmed	The Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate)
title_sort	effect of continuous multistage countercurrent extraction (cmce) propolis extract administration on leydig, and sertoli cells counts, and sperm quality induced with monosodium glutamate (msg) (an experimental study on male wistar rat induced by monosodium glutamate)
publisher	Universitas Sultan Agung Semarang
publishDate	2020
url	https://doaj.org/article/2acac12a5c3745acb376b2d1b813828d
work_keys_str_mv	AT rahmawatirahmawati theeffectofcontinuousmultistagecountercurrentextractioncmcepropolisextractadministrationonleydigandsertolicellscountsandspermqualityinducedwithmonosodiumglutamatemsganexperimentalstudyonmalewistarratinducedbymonosodiumglutamate AT taufiqurrachmannasihun theeffectofcontinuousmultistagecountercurrentextractioncmcepropolisextractadministrationonleydigandsertolicellscountsandspermqualityinducedwithmonosodiumglutamatemsganexperimentalstudyonmalewistarratinducedbymonosodiumglutamate AT israhnantoisradji theeffectofcontinuousmultistagecountercurrentextractioncmcepropolisextractadministrationonleydigandsertolicellscountsandspermqualityinducedwithmonosodiumglutamatemsganexperimentalstudyonmalewistarratinducedbymonosodiumglutamate AT rahmawatirahmawati effectofcontinuousmultistagecountercurrentextractioncmcepropolisextractadministrationonleydigandsertolicellscountsandspermqualityinducedwithmonosodiumglutamatemsganexperimentalstudyonmalewistarratinducedbymonosodiumglutamate AT taufiqurrachmannasihun effectofcontinuousmultistagecountercurrentextractioncmcepropolisextractadministrationonleydigandsertolicellscountsandspermqualityinducedwithmonosodiumglutamatemsganexperimentalstudyonmalewistarratinducedbymonosodiumglutamate AT israhnantoisradji effectofcontinuousmultistagecountercurrentextractioncmcepropolisextractadministrationonleydigandsertolicellscountsandspermqualityinducedwithmonosodiumglutamatemsganexperimentalstudyonmalewistarratinducedbymonosodiumglutamate
_version_	1718413487078637568

The Effect of Continuous Multistage Countercurrent Extraction (CMCE) Propolis Extract Administration on Leydig, and Sertoli Cells Counts, and Sperm Quality Induced With Monosodium Glutamate (MSG) (An experimental study on male Wistar rat induced by monosodium glutamate)

Ejemplares similares