Phototherapy of Brain Tumours Using a Fibre Optic Neurosystem

In this work, a new approach was tested to assess the cellular composition of tissues by time-resolved methods of fluorescence analysis of exogenous and endogenous fluorophores. First of all, the differences in fluorescence kinetics of endogenous fluorophores (coenzymes NADH and FAD) in tumour and i...

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Autores principales:	Yuliya Maklygina, Igor Romanishkin, Aleksej Skobeltsin, Dina Farrakhova, Victor Loschenov
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	fibre optic neurosystem time-resolved laser spectroscopy laser confocal microscopy photosensitizers endogenous fluorophores (NADH, FAD) spectral-resolved microimages of malignant neural tissue tumours Applied optics. Photonics TA1501-1820
Acceso en línea:	https://doaj.org/article/2acb0f05e4fb4439bbec2327b1b90596
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spelling	oai:doaj.org-article:2acb0f05e4fb4439bbec2327b1b905962021-11-25T18:42:58ZPhototherapy of Brain Tumours Using a Fibre Optic Neurosystem10.3390/photonics81104622304-6732https://doaj.org/article/2acb0f05e4fb4439bbec2327b1b905962021-10-01T00:00:00Zhttps://www.mdpi.com/2304-6732/8/11/462https://doaj.org/toc/2304-6732In this work, a new approach was tested to assess the cellular composition of tissues by time-resolved methods of fluorescence analysis of exogenous and endogenous fluorophores. First of all, the differences in fluorescence kinetics of endogenous fluorophores (coenzymes NADH and FAD) in tumour and immunocompetent cells were determined. After that, differences in fluorescence kinetics of photosensitizer 5 ALA-induced protoporphyrin IX were established due to its different metabolism in cells of different phenotypes. Kinetics of photoluminescence of NADH and FAD coenzymes as well as photosensitizer were studied by means of two different methods: time-resolved spectroscopy based on a streak-camera and fibre optic neuroscopy, which served to perform process monitoring and regular fluorescence diagnosis of the probed region. Time-resolved fluorescence microscopy (FLIM) was used as a control technique. Time-resolved spectroscopic fluorescence lifetime analysis was performed on sexually mature female rats induced with glioma C6 brain tumour under in vivo conditions; thus, under conditions where the immune system actively intervenes in the process of oncogenesis. In this regard, the aim of the study was to recognize the cellular composition of the brain tumour tissue, namely the ratio of cancer and immunocompetent cells and their mutual localization. Understanding the role of the immune system thus provides new ways and approaches for further diagnosis and therapy, making tumour-associated immune cells a prime target for modern therapies.Yuliya MaklyginaIgor RomanishkinAleksej SkobeltsinDina FarrakhovaVictor LoschenovMDPI AGarticlefibre optic neurosystemtime-resolved laser spectroscopylaser confocal microscopyphotosensitizersendogenous fluorophores (NADH, FAD)spectral-resolved microimages of malignant neural tissue tumoursApplied optics. PhotonicsTA1501-1820ENPhotonics, Vol 8, Iss 462, p 462 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fibre optic neurosystem time-resolved laser spectroscopy laser confocal microscopy photosensitizers endogenous fluorophores (NADH, FAD) spectral-resolved microimages of malignant neural tissue tumours Applied optics. Photonics TA1501-1820
spellingShingle	fibre optic neurosystem time-resolved laser spectroscopy laser confocal microscopy photosensitizers endogenous fluorophores (NADH, FAD) spectral-resolved microimages of malignant neural tissue tumours Applied optics. Photonics TA1501-1820 Yuliya Maklygina Igor Romanishkin Aleksej Skobeltsin Dina Farrakhova Victor Loschenov Phototherapy of Brain Tumours Using a Fibre Optic Neurosystem
description	In this work, a new approach was tested to assess the cellular composition of tissues by time-resolved methods of fluorescence analysis of exogenous and endogenous fluorophores. First of all, the differences in fluorescence kinetics of endogenous fluorophores (coenzymes NADH and FAD) in tumour and immunocompetent cells were determined. After that, differences in fluorescence kinetics of photosensitizer 5 ALA-induced protoporphyrin IX were established due to its different metabolism in cells of different phenotypes. Kinetics of photoluminescence of NADH and FAD coenzymes as well as photosensitizer were studied by means of two different methods: time-resolved spectroscopy based on a streak-camera and fibre optic neuroscopy, which served to perform process monitoring and regular fluorescence diagnosis of the probed region. Time-resolved fluorescence microscopy (FLIM) was used as a control technique. Time-resolved spectroscopic fluorescence lifetime analysis was performed on sexually mature female rats induced with glioma C6 brain tumour under in vivo conditions; thus, under conditions where the immune system actively intervenes in the process of oncogenesis. In this regard, the aim of the study was to recognize the cellular composition of the brain tumour tissue, namely the ratio of cancer and immunocompetent cells and their mutual localization. Understanding the role of the immune system thus provides new ways and approaches for further diagnosis and therapy, making tumour-associated immune cells a prime target for modern therapies.
format	article
author	Yuliya Maklygina Igor Romanishkin Aleksej Skobeltsin Dina Farrakhova Victor Loschenov
author_facet	Yuliya Maklygina Igor Romanishkin Aleksej Skobeltsin Dina Farrakhova Victor Loschenov
author_sort	Yuliya Maklygina
title	Phototherapy of Brain Tumours Using a Fibre Optic Neurosystem
title_short	Phototherapy of Brain Tumours Using a Fibre Optic Neurosystem
title_full	Phototherapy of Brain Tumours Using a Fibre Optic Neurosystem
title_fullStr	Phototherapy of Brain Tumours Using a Fibre Optic Neurosystem
title_full_unstemmed	Phototherapy of Brain Tumours Using a Fibre Optic Neurosystem
title_sort	phototherapy of brain tumours using a fibre optic neurosystem
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2acb0f05e4fb4439bbec2327b1b90596
work_keys_str_mv	AT yuliyamaklygina phototherapyofbraintumoursusingafibreopticneurosystem AT igorromanishkin phototherapyofbraintumoursusingafibreopticneurosystem AT aleksejskobeltsin phototherapyofbraintumoursusingafibreopticneurosystem AT dinafarrakhova phototherapyofbraintumoursusingafibreopticneurosystem AT victorloschenov phototherapyofbraintumoursusingafibreopticneurosystem
_version_	1718410760632139776

Phototherapy of Brain Tumours Using a Fibre Optic Neurosystem

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