Expression, prognosis value, and immune infiltration of lncRNA ASB16-AS1 identified by pan-cancer analysis

Long-chain non-coding RNA ASB16-AS1 has been proven to be an oncogene for many cancer types. However, the relationship between ASB16-AS1 and immunity is still under studied. This study aims to explore the expression and prognostic potential of ASB16-AS1, and to visualize the relationship between ASB...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Linyong Wu, Wei Liao, Xiaodong Wang, Yujia Zhao, Jinshu Pang, Yuji Chen, Hong Yang, Yun He
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	pan-cancer long-chain non-coding rna asb16-as1 prognosis immune infiltration molecular characteristics Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/2acd64a48a514da09e972e92981218b8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2acd64a48a514da09e972e92981218b8
record_format	dspace
spelling	oai:doaj.org-article:2acd64a48a514da09e972e92981218b82021-11-04T15:51:54ZExpression, prognosis value, and immune infiltration of lncRNA ASB16-AS1 identified by pan-cancer analysis2165-59792165-598710.1080/21655979.2021.1996054https://doaj.org/article/2acd64a48a514da09e972e92981218b82021-10-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1996054https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Long-chain non-coding RNA ASB16-AS1 has been proven to be an oncogene for many cancer types. However, the relationship between ASB16-AS1 and immunity is still under studied. This study aims to explore the expression and prognostic potential of ASB16-AS1, and to visualize the relationship between ASB16-AS1 expression and immune infiltration in pan-cancer. We clarified ASB16-AS1 expression patterns in pan-cancer and its relationship with prognosis through multi-platform and multi-database sources (TCGA, GEO, GTEx, ArrayExpress, and SRA), and verified the function of ASB16-AS1 in liver hepatocellular carcinoma (LIHC). Then, a variety of immune cell content evaluation methods were used to mutually verify the correlation between ASB16-AS1 and immune infiltration. Finally, the relationships between ASB16-AS1 and some molecular characteristics (immune checkpoints, tumor mutation burden, microsatellite instability, oncogenic signaling pathways) were further explored. In terms of comprehensive analysis, compared with non-tumor tissues, ASB16-AS1 was highly expressed in tumor tissues, and indicated the value of poor prognosis in multiple cancer types. Functional assays, such as CCK8 assay, transwell assay and wound scratch assay, verified that high ASB16-AS1 expression promoted tumor progression in LIHC. ASB16-AS1 was positively correlated with B cells, T cell CD4+ and T cell CD8+ in most cancer types, and negatively correlated with macrophages, dendritic cells and neutrophils in some cancer types, especially in neutrophils. In addition, there were different interaction modes between ASB16-AS1 and molecular features, such as the relationship with oncogenic signaling pathways, showing that the high ASB16-AS1 expression was related to alterations in oncogenic signaling pathways. Our study emphasizes that ASB16-AS1 is a potential pan-cancer prognostic marker, whichs is associated with the immune infiltration in multiple cancer types.Linyong WuWei LiaoXiaodong WangYujia ZhaoJinshu PangYuji ChenHong YangYun HeTaylor & Francis Grouparticlepan-cancerlong-chain non-coding rna asb16-as1prognosisimmune infiltrationmolecular characteristicsBiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pan-cancer long-chain non-coding rna asb16-as1 prognosis immune infiltration molecular characteristics Biotechnology TP248.13-248.65
spellingShingle	pan-cancer long-chain non-coding rna asb16-as1 prognosis immune infiltration molecular characteristics Biotechnology TP248.13-248.65 Linyong Wu Wei Liao Xiaodong Wang Yujia Zhao Jinshu Pang Yuji Chen Hong Yang Yun He Expression, prognosis value, and immune infiltration of lncRNA ASB16-AS1 identified by pan-cancer analysis
description	Long-chain non-coding RNA ASB16-AS1 has been proven to be an oncogene for many cancer types. However, the relationship between ASB16-AS1 and immunity is still under studied. This study aims to explore the expression and prognostic potential of ASB16-AS1, and to visualize the relationship between ASB16-AS1 expression and immune infiltration in pan-cancer. We clarified ASB16-AS1 expression patterns in pan-cancer and its relationship with prognosis through multi-platform and multi-database sources (TCGA, GEO, GTEx, ArrayExpress, and SRA), and verified the function of ASB16-AS1 in liver hepatocellular carcinoma (LIHC). Then, a variety of immune cell content evaluation methods were used to mutually verify the correlation between ASB16-AS1 and immune infiltration. Finally, the relationships between ASB16-AS1 and some molecular characteristics (immune checkpoints, tumor mutation burden, microsatellite instability, oncogenic signaling pathways) were further explored. In terms of comprehensive analysis, compared with non-tumor tissues, ASB16-AS1 was highly expressed in tumor tissues, and indicated the value of poor prognosis in multiple cancer types. Functional assays, such as CCK8 assay, transwell assay and wound scratch assay, verified that high ASB16-AS1 expression promoted tumor progression in LIHC. ASB16-AS1 was positively correlated with B cells, T cell CD4+ and T cell CD8+ in most cancer types, and negatively correlated with macrophages, dendritic cells and neutrophils in some cancer types, especially in neutrophils. In addition, there were different interaction modes between ASB16-AS1 and molecular features, such as the relationship with oncogenic signaling pathways, showing that the high ASB16-AS1 expression was related to alterations in oncogenic signaling pathways. Our study emphasizes that ASB16-AS1 is a potential pan-cancer prognostic marker, whichs is associated with the immune infiltration in multiple cancer types.
format	article
author	Linyong Wu Wei Liao Xiaodong Wang Yujia Zhao Jinshu Pang Yuji Chen Hong Yang Yun He
author_facet	Linyong Wu Wei Liao Xiaodong Wang Yujia Zhao Jinshu Pang Yuji Chen Hong Yang Yun He
author_sort	Linyong Wu
title	Expression, prognosis value, and immune infiltration of lncRNA ASB16-AS1 identified by pan-cancer analysis
title_short	Expression, prognosis value, and immune infiltration of lncRNA ASB16-AS1 identified by pan-cancer analysis
title_full	Expression, prognosis value, and immune infiltration of lncRNA ASB16-AS1 identified by pan-cancer analysis
title_fullStr	Expression, prognosis value, and immune infiltration of lncRNA ASB16-AS1 identified by pan-cancer analysis
title_full_unstemmed	Expression, prognosis value, and immune infiltration of lncRNA ASB16-AS1 identified by pan-cancer analysis
title_sort	expression, prognosis value, and immune infiltration of lncrna asb16-as1 identified by pan-cancer analysis
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/2acd64a48a514da09e972e92981218b8
work_keys_str_mv	AT linyongwu expressionprognosisvalueandimmuneinfiltrationoflncrnaasb16as1identifiedbypancanceranalysis AT weiliao expressionprognosisvalueandimmuneinfiltrationoflncrnaasb16as1identifiedbypancanceranalysis AT xiaodongwang expressionprognosisvalueandimmuneinfiltrationoflncrnaasb16as1identifiedbypancanceranalysis AT yujiazhao expressionprognosisvalueandimmuneinfiltrationoflncrnaasb16as1identifiedbypancanceranalysis AT jinshupang expressionprognosisvalueandimmuneinfiltrationoflncrnaasb16as1identifiedbypancanceranalysis AT yujichen expressionprognosisvalueandimmuneinfiltrationoflncrnaasb16as1identifiedbypancanceranalysis AT hongyang expressionprognosisvalueandimmuneinfiltrationoflncrnaasb16as1identifiedbypancanceranalysis AT yunhe expressionprognosisvalueandimmuneinfiltrationoflncrnaasb16as1identifiedbypancanceranalysis
_version_	1718444705045282816

Expression, prognosis value, and immune infiltration of lncRNA ASB16-AS1 identified by pan-cancer analysis

Ejemplares similares