Detection of qacEΔ1, qacG, qacE, qacF resistance genes in Escherichia coli producing broad-spectrum beta-lactamases to benzalkonium chloride

BACKGROUND AND OBJECTIVE: The resistance genes of quaternary ammonium compounds(qac) play an important role in the resistance of gram-negative bacteria producing broad-spectrum beta-lactamases to disinfectants. The aim of this study was detection of qacEΔ1, qacG, qacE, qacF resistance genes in Esche...

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Autores principales:	F Hadadi, E Ghaznavirad, A Almasi-Hashiani, H Abtahi
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2019
Materias:	escherichia coli beta-lactamase benzalkonium chloride disinfectant Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2ade6fbae18a44feaa5561ece620f413
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spelling	oai:doaj.org-article:2ade6fbae18a44feaa5561ece620f4132021-11-10T08:30:37ZDetection of qacEΔ1, qacG, qacE, qacF resistance genes in Escherichia coli producing broad-spectrum beta-lactamases to benzalkonium chloride1561-41072251-7170https://doaj.org/article/2ade6fbae18a44feaa5561ece620f4132019-12-01T00:00:00Zhttp://jbums.org/article-1-8484-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: The resistance genes of quaternary ammonium compounds(qac) play an important role in the resistance of gram-negative bacteria producing broad-spectrum beta-lactamases to disinfectants. The aim of this study was detection of qacEΔ1, qacG, qacE, qacF resistance genes in Escherichia coli producing broad-spectrum beta-lactamases to benzalkonium chloride. METHODS: This study cross sectional-descriptive was conducted on 150 clinical samples of selected hospitals in Arak. ESBL strains were identified by using phenotypic methods of disc diffusion and combinatory disc method and evaluating the SHV, TEM, CTXM1 genes by genotyping method. The PCR was performed to determine the resistance genes qacEΔ1, qacG, qacE and qacF.The electrophoresis of PCR products and the MIC of benzalkonium chloride were relative to E. coli producing ESBL. Antibiotic pattern of Escherichia coli (ESBL), quadruple ammonium resistance genes and benzalkonium chloride MIC were also investigated. FINDINGS: This study showed that 60% of Escherichia coli were ESBL producer. The qacEΔ1 genes were observed in all of them and qacE, qacF, qacG genes were not found in any of the strains. The strains had MIC range from 32 to 64 mg/l for benzalkonium chloride. Resistance to carbapenems (33.33%) was observed. CONCLUSION: This study showed that qacEΔ1 resistance gene and resistance to disinfectant benzalkonium chloride increased. Also increased resistance to the antibiotics studied were observed in E. coli ESBL strains.F HadadiE GhaznaviradA Almasi-HashianiH AbtahiBabol University of Medical Sciencesarticleescherichia colibeta-lactamasebenzalkonium chloridedisinfectantMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 21, Iss 1, Pp 286-292 (2019)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	escherichia coli beta-lactamase benzalkonium chloride disinfectant Medicine R Medicine (General) R5-920
spellingShingle	escherichia coli beta-lactamase benzalkonium chloride disinfectant Medicine R Medicine (General) R5-920 F Hadadi E Ghaznavirad A Almasi-Hashiani H Abtahi Detection of qacEΔ1, qacG, qacE, qacF resistance genes in Escherichia coli producing broad-spectrum beta-lactamases to benzalkonium chloride
description	BACKGROUND AND OBJECTIVE: The resistance genes of quaternary ammonium compounds(qac) play an important role in the resistance of gram-negative bacteria producing broad-spectrum beta-lactamases to disinfectants. The aim of this study was detection of qacEΔ1, qacG, qacE, qacF resistance genes in Escherichia coli producing broad-spectrum beta-lactamases to benzalkonium chloride. METHODS: This study cross sectional-descriptive was conducted on 150 clinical samples of selected hospitals in Arak. ESBL strains were identified by using phenotypic methods of disc diffusion and combinatory disc method and evaluating the SHV, TEM, CTXM1 genes by genotyping method. The PCR was performed to determine the resistance genes qacEΔ1, qacG, qacE and qacF.The electrophoresis of PCR products and the MIC of benzalkonium chloride were relative to E. coli producing ESBL. Antibiotic pattern of Escherichia coli (ESBL), quadruple ammonium resistance genes and benzalkonium chloride MIC were also investigated. FINDINGS: This study showed that 60% of Escherichia coli were ESBL producer. The qacEΔ1 genes were observed in all of them and qacE, qacF, qacG genes were not found in any of the strains. The strains had MIC range from 32 to 64 mg/l for benzalkonium chloride. Resistance to carbapenems (33.33%) was observed. CONCLUSION: This study showed that qacEΔ1 resistance gene and resistance to disinfectant benzalkonium chloride increased. Also increased resistance to the antibiotics studied were observed in E. coli ESBL strains.
format	article
author	F Hadadi E Ghaznavirad A Almasi-Hashiani H Abtahi
author_facet	F Hadadi E Ghaznavirad A Almasi-Hashiani H Abtahi
author_sort	F Hadadi
title	Detection of qacEΔ1, qacG, qacE, qacF resistance genes in Escherichia coli producing broad-spectrum beta-lactamases to benzalkonium chloride
title_short	Detection of qacEΔ1, qacG, qacE, qacF resistance genes in Escherichia coli producing broad-spectrum beta-lactamases to benzalkonium chloride
title_full	Detection of qacEΔ1, qacG, qacE, qacF resistance genes in Escherichia coli producing broad-spectrum beta-lactamases to benzalkonium chloride
title_fullStr	Detection of qacEΔ1, qacG, qacE, qacF resistance genes in Escherichia coli producing broad-spectrum beta-lactamases to benzalkonium chloride
title_full_unstemmed	Detection of qacEΔ1, qacG, qacE, qacF resistance genes in Escherichia coli producing broad-spectrum beta-lactamases to benzalkonium chloride
title_sort	detection of qaceδ1, qacg, qace, qacf resistance genes in escherichia coli producing broad-spectrum beta-lactamases to benzalkonium chloride
publisher	Babol University of Medical Sciences
publishDate	2019
url	https://doaj.org/article/2ade6fbae18a44feaa5561ece620f413
work_keys_str_mv	AT fhadadi detectionofqaced1qacgqaceqacfresistancegenesinescherichiacoliproducingbroadspectrumbetalactamasestobenzalkoniumchloride AT eghaznavirad detectionofqaced1qacgqaceqacfresistancegenesinescherichiacoliproducingbroadspectrumbetalactamasestobenzalkoniumchloride AT aalmasihashiani detectionofqaced1qacgqaceqacfresistancegenesinescherichiacoliproducingbroadspectrumbetalactamasestobenzalkoniumchloride AT habtahi detectionofqaced1qacgqaceqacfresistancegenesinescherichiacoliproducingbroadspectrumbetalactamasestobenzalkoniumchloride
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Detection of qacEΔ1, qacG, qacE, qacF resistance genes in Escherichia coli producing broad-spectrum beta-lactamases to benzalkonium chloride

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