Optimization of Quantitative Analysis of Biofilm Cell from Pipe Materials

The quantitative analysis of biofilm can be used not only to assess the microbiological stability of tap water but also on its basis can assess: the degree of colonization of materials by bacterial cells, the rate of biofilm formation on the surfaces of pipes and determine their composition and numb...

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Autores principales:	Dorota Papciak, Andżelika Domoń, Monika Zdeb, Agata Skwarczyńska-Wojsa, Janusz Konkol
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	pipe material biofilm measurement water supply system environmental engineering Engineering (General). Civil engineering (General) TA1-2040
Acceso en línea:	https://doaj.org/article/2adfc2e2bfd24fcb8c6f51a8a5734d94
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spelling	oai:doaj.org-article:2adfc2e2bfd24fcb8c6f51a8a5734d942021-11-25T17:15:35ZOptimization of Quantitative Analysis of Biofilm Cell from Pipe Materials10.3390/coatings111112862079-6412https://doaj.org/article/2adfc2e2bfd24fcb8c6f51a8a5734d942021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6412/11/11/1286https://doaj.org/toc/2079-6412The quantitative analysis of biofilm can be used not only to assess the microbiological stability of tap water but also on its basis can assess: the degree of colonization of materials by bacterial cells, the rate of biofilm formation on the surfaces of pipes and determine their composition and number. The article presents the results of research on the development of an effective method of biofilm detachment from the surface of the galvanized steel. The number of biofilm cells was determined by methods: (1) luminometric ATP determination, (2) flow cytometry and (3) heterotrophic plate counts (HPC). The presence of the biofilm was confirmed by SEM and fractal analysis. The analysis of the obtained results showed that the most effective method of detaching the biofilm cells from the galvanized steel surface was the mechanical separation with a sterile cotton swab. The variant with the use of a sterile swab enables rapid collection of the biofilm from the surface of the ducts forming internal installations or water supply networks. Due to the simplicity and speed of obtaining results, the luminometric ATP measurement has been established as the best method for the quantification of biofilm cells. The results of this study were intended to provide reliable and useful data on the quantification of biofilm cells.Dorota PapciakAndżelika DomońMonika ZdebAgata Skwarczyńska-WojsaJanusz KonkolMDPI AGarticlepipe materialbiofilm measurementwater supply systemenvironmental engineeringEngineering (General). Civil engineering (General)TA1-2040ENCoatings, Vol 11, Iss 1286, p 1286 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	pipe material biofilm measurement water supply system environmental engineering Engineering (General). Civil engineering (General) TA1-2040
spellingShingle	pipe material biofilm measurement water supply system environmental engineering Engineering (General). Civil engineering (General) TA1-2040 Dorota Papciak Andżelika Domoń Monika Zdeb Agata Skwarczyńska-Wojsa Janusz Konkol Optimization of Quantitative Analysis of Biofilm Cell from Pipe Materials
description	The quantitative analysis of biofilm can be used not only to assess the microbiological stability of tap water but also on its basis can assess: the degree of colonization of materials by bacterial cells, the rate of biofilm formation on the surfaces of pipes and determine their composition and number. The article presents the results of research on the development of an effective method of biofilm detachment from the surface of the galvanized steel. The number of biofilm cells was determined by methods: (1) luminometric ATP determination, (2) flow cytometry and (3) heterotrophic plate counts (HPC). The presence of the biofilm was confirmed by SEM and fractal analysis. The analysis of the obtained results showed that the most effective method of detaching the biofilm cells from the galvanized steel surface was the mechanical separation with a sterile cotton swab. The variant with the use of a sterile swab enables rapid collection of the biofilm from the surface of the ducts forming internal installations or water supply networks. Due to the simplicity and speed of obtaining results, the luminometric ATP measurement has been established as the best method for the quantification of biofilm cells. The results of this study were intended to provide reliable and useful data on the quantification of biofilm cells.
format	article
author	Dorota Papciak Andżelika Domoń Monika Zdeb Agata Skwarczyńska-Wojsa Janusz Konkol
author_facet	Dorota Papciak Andżelika Domoń Monika Zdeb Agata Skwarczyńska-Wojsa Janusz Konkol
author_sort	Dorota Papciak
title	Optimization of Quantitative Analysis of Biofilm Cell from Pipe Materials
title_short	Optimization of Quantitative Analysis of Biofilm Cell from Pipe Materials
title_full	Optimization of Quantitative Analysis of Biofilm Cell from Pipe Materials
title_fullStr	Optimization of Quantitative Analysis of Biofilm Cell from Pipe Materials
title_full_unstemmed	Optimization of Quantitative Analysis of Biofilm Cell from Pipe Materials
title_sort	optimization of quantitative analysis of biofilm cell from pipe materials
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2adfc2e2bfd24fcb8c6f51a8a5734d94
work_keys_str_mv	AT dorotapapciak optimizationofquantitativeanalysisofbiofilmcellfrompipematerials AT andzelikadomon optimizationofquantitativeanalysisofbiofilmcellfrompipematerials AT monikazdeb optimizationofquantitativeanalysisofbiofilmcellfrompipematerials AT agataskwarczynskawojsa optimizationofquantitativeanalysisofbiofilmcellfrompipematerials AT januszkonkol optimizationofquantitativeanalysisofbiofilmcellfrompipematerials
_version_	1718412579197419520

Optimization of Quantitative Analysis of Biofilm Cell from Pipe Materials

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