Student-Centered Microbioassay Laboratory Activity Utilizing Bioluminescent Bacteria

Student-centered teaching allows students to be actively engaged in hands-on, minds-on activities that emphasize creativity and collaboration, enabling them to ask questions and design their own investigations to real-world problems. One such problem is water contamination, which causes human health...

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Autores principales:	Kerri Younkin, Christina Romano
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/2af07797e8f44158a49e87af1ff1dd57
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spelling	oai:doaj.org-article:2af07797e8f44158a49e87af1ff1dd572021-11-15T15:04:10ZStudent-Centered Microbioassay Laboratory Activity Utilizing Bioluminescent Bacteria10.1128/jmbe.v19i2.13731935-78851935-7877https://doaj.org/article/2af07797e8f44158a49e87af1ff1dd572018-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v19i2.1373https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Student-centered teaching allows students to be actively engaged in hands-on, minds-on activities that emphasize creativity and collaboration, enabling them to ask questions and design their own investigations to real-world problems. One such problem is water contamination, which causes human health and environmental issues. However, chemical water quality testing for pollutants can be timely and expensive. In addition to chemical testing, researchers have developed assays using unicellular organisms to determine which pollutants are present and in what concentrations. In this three-hour laboratory activity, high school students and undergraduate biology or microbiology students work in pairs to help a fictional company develop a water quality microbioassay. Students design their own laboratory protocols to test the reaction of a bioluminescent bacterial species (i.e., Photobacterium phosphoreum or Aliivibrio fischeri) to exposure of common aquatic pollutants such as fertilizer, household cleaners, and motor oil. During this laboratory activity, students apply previously learned components of experimental design, including positive and negative controls, constants, and experimental groups. In addition, students gain experience writing a scientific explanation for a recommendation regarding the bioluminescent bacteria’s suitability in a bioassay. Pre- and post-evaluation data revealed that students were successful in achieving the activity’s objectives as well as in designing their investigations and writing their protocols using scaffolds within the lesson.Kerri YounkinChristina RomanoAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 19, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Kerri Younkin Christina Romano Student-Centered Microbioassay Laboratory Activity Utilizing Bioluminescent Bacteria
description	Student-centered teaching allows students to be actively engaged in hands-on, minds-on activities that emphasize creativity and collaboration, enabling them to ask questions and design their own investigations to real-world problems. One such problem is water contamination, which causes human health and environmental issues. However, chemical water quality testing for pollutants can be timely and expensive. In addition to chemical testing, researchers have developed assays using unicellular organisms to determine which pollutants are present and in what concentrations. In this three-hour laboratory activity, high school students and undergraduate biology or microbiology students work in pairs to help a fictional company develop a water quality microbioassay. Students design their own laboratory protocols to test the reaction of a bioluminescent bacterial species (i.e., Photobacterium phosphoreum or Aliivibrio fischeri) to exposure of common aquatic pollutants such as fertilizer, household cleaners, and motor oil. During this laboratory activity, students apply previously learned components of experimental design, including positive and negative controls, constants, and experimental groups. In addition, students gain experience writing a scientific explanation for a recommendation regarding the bioluminescent bacteria’s suitability in a bioassay. Pre- and post-evaluation data revealed that students were successful in achieving the activity’s objectives as well as in designing their investigations and writing their protocols using scaffolds within the lesson.
format	article
author	Kerri Younkin Christina Romano
author_facet	Kerri Younkin Christina Romano
author_sort	Kerri Younkin
title	Student-Centered Microbioassay Laboratory Activity Utilizing Bioluminescent Bacteria
title_short	Student-Centered Microbioassay Laboratory Activity Utilizing Bioluminescent Bacteria
title_full	Student-Centered Microbioassay Laboratory Activity Utilizing Bioluminescent Bacteria
title_fullStr	Student-Centered Microbioassay Laboratory Activity Utilizing Bioluminescent Bacteria
title_full_unstemmed	Student-Centered Microbioassay Laboratory Activity Utilizing Bioluminescent Bacteria
title_sort	student-centered microbioassay laboratory activity utilizing bioluminescent bacteria
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/2af07797e8f44158a49e87af1ff1dd57
work_keys_str_mv	AT kerriyounkin studentcenteredmicrobioassaylaboratoryactivityutilizingbioluminescentbacteria AT christinaromano studentcenteredmicrobioassaylaboratoryactivityutilizingbioluminescentbacteria
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Student-Centered Microbioassay Laboratory Activity Utilizing Bioluminescent Bacteria

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