Novel approach for accurate tissue-based protein colocalization and proximity microscopy

Abstract Fluorescence colocalization microscopy is frequently used to assess potential links between distinct molecules; however, this method can lead to striking false-positive results and erroneous conclusions. Here we developed a novel approach with more sophisticated mathematical colocalization...

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Autores principales:	Mirjam I. Lutz, Carmen Schwaiger, Bernhard Hochreiter, Gabor G. Kovacs, Johannes A. Schmid
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2af7528ddb9b4f7ebaf9ee2b229fe643
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spelling	oai:doaj.org-article:2af7528ddb9b4f7ebaf9ee2b229fe6432021-12-02T15:06:11ZNovel approach for accurate tissue-based protein colocalization and proximity microscopy10.1038/s41598-017-02735-82045-2322https://doaj.org/article/2af7528ddb9b4f7ebaf9ee2b229fe6432017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02735-8https://doaj.org/toc/2045-2322Abstract Fluorescence colocalization microscopy is frequently used to assess potential links between distinct molecules; however, this method can lead to striking false-positive results and erroneous conclusions. Here we developed a novel approach with more sophisticated mathematical colocalization analyses together with visualization of physical proximity using fluorescence resonance energy transfer (FRET). To verify our results we used the proximity ligation assay (PLA). With these methods we could demonstrate that distinct neurodegeneration-related proteins either not or only rarely interact in human brain tissue.Mirjam I. LutzCarmen SchwaigerBernhard HochreiterGabor G. KovacsJohannes A. SchmidNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mirjam I. Lutz Carmen Schwaiger Bernhard Hochreiter Gabor G. Kovacs Johannes A. Schmid Novel approach for accurate tissue-based protein colocalization and proximity microscopy
description	Abstract Fluorescence colocalization microscopy is frequently used to assess potential links between distinct molecules; however, this method can lead to striking false-positive results and erroneous conclusions. Here we developed a novel approach with more sophisticated mathematical colocalization analyses together with visualization of physical proximity using fluorescence resonance energy transfer (FRET). To verify our results we used the proximity ligation assay (PLA). With these methods we could demonstrate that distinct neurodegeneration-related proteins either not or only rarely interact in human brain tissue.
format	article
author	Mirjam I. Lutz Carmen Schwaiger Bernhard Hochreiter Gabor G. Kovacs Johannes A. Schmid
author_facet	Mirjam I. Lutz Carmen Schwaiger Bernhard Hochreiter Gabor G. Kovacs Johannes A. Schmid
author_sort	Mirjam I. Lutz
title	Novel approach for accurate tissue-based protein colocalization and proximity microscopy
title_short	Novel approach for accurate tissue-based protein colocalization and proximity microscopy
title_full	Novel approach for accurate tissue-based protein colocalization and proximity microscopy
title_fullStr	Novel approach for accurate tissue-based protein colocalization and proximity microscopy
title_full_unstemmed	Novel approach for accurate tissue-based protein colocalization and proximity microscopy
title_sort	novel approach for accurate tissue-based protein colocalization and proximity microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2af7528ddb9b4f7ebaf9ee2b229fe643
work_keys_str_mv	AT mirjamilutz novelapproachforaccuratetissuebasedproteincolocalizationandproximitymicroscopy AT carmenschwaiger novelapproachforaccuratetissuebasedproteincolocalizationandproximitymicroscopy AT bernhardhochreiter novelapproachforaccuratetissuebasedproteincolocalizationandproximitymicroscopy AT gaborgkovacs novelapproachforaccuratetissuebasedproteincolocalizationandproximitymicroscopy AT johannesaschmid novelapproachforaccuratetissuebasedproteincolocalizationandproximitymicroscopy
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Novel approach for accurate tissue-based protein colocalization and proximity microscopy

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