EFFECTS OF STATINS UPON ANTIGEN-SPECIFIC LYMPHOCYTE ACTIVATION IN PATIENTS WITH RHEUMATOID ARTHRITIS: AN IN VITRO STUDY

Abstract. Collagen type II (Col II) is among the proposed candidate autoantigens initiaiting rheumatoid arthritis (RA). Statins have been shown to have anti-inflammatory and immunomodulating properties. Mechanisms of statin effects upon the autoantigen-induced T lymphocyte activation in RA patients...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	I. V. Shirinsky, V. S. Shirinsky
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	rheumatoid arthritis statins in vitro Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/2afaecdd107346b6b6318210a5e95027
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2afaecdd107346b6b6318210a5e95027
record_format	dspace
spelling	oai:doaj.org-article:2afaecdd107346b6b6318210a5e950272021-11-18T08:03:38ZEFFECTS OF STATINS UPON ANTIGEN-SPECIFIC LYMPHOCYTE ACTIVATION IN PATIENTS WITH RHEUMATOID ARTHRITIS: AN IN VITRO STUDY1563-06252313-741X10.15789/1563-0625-2008-1-77-80https://doaj.org/article/2afaecdd107346b6b6318210a5e950272014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/84https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. Collagen type II (Col II) is among the proposed candidate autoantigens initiaiting rheumatoid arthritis (RA). Statins have been shown to have anti-inflammatory and immunomodulating properties. Mechanisms of statin effects upon the autoantigen-induced T lymphocyte activation in RA patients are still unknown. The aim of present study was to evaluate the effects of mevastatin upon Col II- and anti-CD3-induced (PBMC) activation of peripheral blood mononuclear cells (PBMC) from RA patients. PBMC from active RA patients (DAS28 – 6.6±0.64) were stimulated with antibodies specific for either anti-CD3 or anti-Col II. The cultured cells were treated with mevastatin at different concentrations. Mevalonic acid, geranylgeranyl pyrophosphate (GGpp) and farnesyl pyrophosphate (Fpp) were added to cultures. Mevastatin at concentration of 10 μM caused significant reduction of Col II-induced PBMC proliferation and of IFNγ production. These mevastatin effects were partially reversible by addition of mevalonic acid, GGpp, and Fpp. Mevastatin did not influence the anti-CD3 induced PBMC activation. In conclusion, mevastatin is able to suppress antigen-specific T-cell stimulation in RA patients, due to decreased production of mevalonic acid metabolites.I. V. ShirinskyV. S. ShirinskySPb RAACIarticlerheumatoid arthritisstatinsin vitroImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 10, Iss 1, Pp 77-80 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	rheumatoid arthritis statins in vitro Immunologic diseases. Allergy RC581-607
spellingShingle	rheumatoid arthritis statins in vitro Immunologic diseases. Allergy RC581-607 I. V. Shirinsky V. S. Shirinsky EFFECTS OF STATINS UPON ANTIGEN-SPECIFIC LYMPHOCYTE ACTIVATION IN PATIENTS WITH RHEUMATOID ARTHRITIS: AN IN VITRO STUDY
description	Abstract. Collagen type II (Col II) is among the proposed candidate autoantigens initiaiting rheumatoid arthritis (RA). Statins have been shown to have anti-inflammatory and immunomodulating properties. Mechanisms of statin effects upon the autoantigen-induced T lymphocyte activation in RA patients are still unknown. The aim of present study was to evaluate the effects of mevastatin upon Col II- and anti-CD3-induced (PBMC) activation of peripheral blood mononuclear cells (PBMC) from RA patients. PBMC from active RA patients (DAS28 – 6.6±0.64) were stimulated with antibodies specific for either anti-CD3 or anti-Col II. The cultured cells were treated with mevastatin at different concentrations. Mevalonic acid, geranylgeranyl pyrophosphate (GGpp) and farnesyl pyrophosphate (Fpp) were added to cultures. Mevastatin at concentration of 10 μM caused significant reduction of Col II-induced PBMC proliferation and of IFNγ production. These mevastatin effects were partially reversible by addition of mevalonic acid, GGpp, and Fpp. Mevastatin did not influence the anti-CD3 induced PBMC activation. In conclusion, mevastatin is able to suppress antigen-specific T-cell stimulation in RA patients, due to decreased production of mevalonic acid metabolites.
format	article
author	I. V. Shirinsky V. S. Shirinsky
author_facet	I. V. Shirinsky V. S. Shirinsky
author_sort	I. V. Shirinsky
title	EFFECTS OF STATINS UPON ANTIGEN-SPECIFIC LYMPHOCYTE ACTIVATION IN PATIENTS WITH RHEUMATOID ARTHRITIS: AN IN VITRO STUDY
title_short	EFFECTS OF STATINS UPON ANTIGEN-SPECIFIC LYMPHOCYTE ACTIVATION IN PATIENTS WITH RHEUMATOID ARTHRITIS: AN IN VITRO STUDY
title_full	EFFECTS OF STATINS UPON ANTIGEN-SPECIFIC LYMPHOCYTE ACTIVATION IN PATIENTS WITH RHEUMATOID ARTHRITIS: AN IN VITRO STUDY
title_fullStr	EFFECTS OF STATINS UPON ANTIGEN-SPECIFIC LYMPHOCYTE ACTIVATION IN PATIENTS WITH RHEUMATOID ARTHRITIS: AN IN VITRO STUDY
title_full_unstemmed	EFFECTS OF STATINS UPON ANTIGEN-SPECIFIC LYMPHOCYTE ACTIVATION IN PATIENTS WITH RHEUMATOID ARTHRITIS: AN IN VITRO STUDY
title_sort	effects of statins upon antigen-specific lymphocyte activation in patients with rheumatoid arthritis: an in vitro study
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/2afaecdd107346b6b6318210a5e95027
work_keys_str_mv	AT ivshirinsky effectsofstatinsuponantigenspecificlymphocyteactivationinpatientswithrheumatoidarthritisaninvitrostudy AT vsshirinsky effectsofstatinsuponantigenspecificlymphocyteactivationinpatientswithrheumatoidarthritisaninvitrostudy
_version_	1718422532221042688

EFFECTS OF STATINS UPON ANTIGEN-SPECIFIC LYMPHOCYTE ACTIVATION IN PATIENTS WITH RHEUMATOID ARTHRITIS: AN IN VITRO STUDY

Ejemplares similares