A thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate

Abstract Acetoin (3-hydroxy-2-butanone) is an important bio-based platform chemical with wide applications. In vitro enzyme catalysed synthesis exhibits great feasibility in the production of chemicals with high purity. In the present work, a synthetic pathway involving a two-step continuous reactio...

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Autores principales:	Xiaojing Jia, Ying Liu, Yejun Han
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b063dd66e784cc2b8fc18b51a41ebbe
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spelling	oai:doaj.org-article:2b063dd66e784cc2b8fc18b51a41ebbe2021-12-02T11:40:30ZA thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate10.1038/s41598-017-04684-82045-2322https://doaj.org/article/2b063dd66e784cc2b8fc18b51a41ebbe2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04684-8https://doaj.org/toc/2045-2322Abstract Acetoin (3-hydroxy-2-butanone) is an important bio-based platform chemical with wide applications. In vitro enzyme catalysed synthesis exhibits great feasibility in the production of chemicals with high purity. In the present work, a synthetic pathway involving a two-step continuous reaction was constructed in vitro for acetoin production from pyruvate at improved temperature. Thermostable candidates, acetolactate synthase (coAHASL1 and coAHASL2 from Caldicellulosiruptor owensensis OL) and α-acetolactate decarboxylase (bsALDC from Bacillus subtilis IPE5-4) were cloned, heterologously expressed, and characterized. All the enzymes showed maximum activities at 65–70 °C and pH of 6.5. Enzyme kinetics analysis showed that coAHASL1 had a higher activity but lower affinity against pyruvate than that of coAHASL2. In addition, the activities of coAHASL1 and bsALDC were promoted by Mn2+ and NADPH. The cascade enzymatic reaction was optimized by using coAHASL1 and bsALDC based on their kinetic properties. Under optimal conditions, a maximum concentration of 3.36 ± 0.26 mM acetoin was produced from 10 mM pyruvate after reaction for 24 h at 65 °C. The productivity of acetoin was 0.14 mM h−1, and the yield was 67.80% compared with the theoretical value. The results confirmed the feasibility of synthesis of acetoin from pyruvate with a cell-free enzyme catalysed system at improved temperature.Xiaojing JiaYing LiuYejun HanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaojing Jia Ying Liu Yejun Han A thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate
description	Abstract Acetoin (3-hydroxy-2-butanone) is an important bio-based platform chemical with wide applications. In vitro enzyme catalysed synthesis exhibits great feasibility in the production of chemicals with high purity. In the present work, a synthetic pathway involving a two-step continuous reaction was constructed in vitro for acetoin production from pyruvate at improved temperature. Thermostable candidates, acetolactate synthase (coAHASL1 and coAHASL2 from Caldicellulosiruptor owensensis OL) and α-acetolactate decarboxylase (bsALDC from Bacillus subtilis IPE5-4) were cloned, heterologously expressed, and characterized. All the enzymes showed maximum activities at 65–70 °C and pH of 6.5. Enzyme kinetics analysis showed that coAHASL1 had a higher activity but lower affinity against pyruvate than that of coAHASL2. In addition, the activities of coAHASL1 and bsALDC were promoted by Mn2+ and NADPH. The cascade enzymatic reaction was optimized by using coAHASL1 and bsALDC based on their kinetic properties. Under optimal conditions, a maximum concentration of 3.36 ± 0.26 mM acetoin was produced from 10 mM pyruvate after reaction for 24 h at 65 °C. The productivity of acetoin was 0.14 mM h−1, and the yield was 67.80% compared with the theoretical value. The results confirmed the feasibility of synthesis of acetoin from pyruvate with a cell-free enzyme catalysed system at improved temperature.
format	article
author	Xiaojing Jia Ying Liu Yejun Han
author_facet	Xiaojing Jia Ying Liu Yejun Han
author_sort	Xiaojing Jia
title	A thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate
title_short	A thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate
title_full	A thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate
title_fullStr	A thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate
title_full_unstemmed	A thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate
title_sort	thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2b063dd66e784cc2b8fc18b51a41ebbe
work_keys_str_mv	AT xiaojingjia athermophiliccellfreecascadeenzymaticreactionforacetoinsynthesisfrompyruvate AT yingliu athermophiliccellfreecascadeenzymaticreactionforacetoinsynthesisfrompyruvate AT yejunhan athermophiliccellfreecascadeenzymaticreactionforacetoinsynthesisfrompyruvate AT xiaojingjia thermophiliccellfreecascadeenzymaticreactionforacetoinsynthesisfrompyruvate AT yingliu thermophiliccellfreecascadeenzymaticreactionforacetoinsynthesisfrompyruvate AT yejunhan thermophiliccellfreecascadeenzymaticreactionforacetoinsynthesisfrompyruvate
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A thermophilic cell-free cascade enzymatic reaction for acetoin synthesis from pyruvate

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