Comparative analysis of gene expression level by quantitative real-time PCR has limited application in objects with different morphology.

qRT-PCR is a generally acknowledged method for gene expression analysis due to its precision and reproducibility. However, it is well known that the accuracy of qRT-PCR data varies greatly depending on the experimental design and data analysis. Recently, a set of guidelines has been proposed that ai...

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Autores principales:	Natalia V Demidenko, Aleksey A Penin
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b087ac5091f40879687cb36006d1c25
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spelling	oai:doaj.org-article:2b087ac5091f40879687cb36006d1c252021-11-18T07:16:52ZComparative analysis of gene expression level by quantitative real-time PCR has limited application in objects with different morphology.1932-620310.1371/journal.pone.0038161https://doaj.org/article/2b087ac5091f40879687cb36006d1c252012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22666472/?tool=EBIhttps://doaj.org/toc/1932-6203qRT-PCR is a generally acknowledged method for gene expression analysis due to its precision and reproducibility. However, it is well known that the accuracy of qRT-PCR data varies greatly depending on the experimental design and data analysis. Recently, a set of guidelines has been proposed that aims to improve the reliability of qRT-PCR. However, there are additional factors that have not been taken into consideration in these guidelines that can seriously affect the data obtained using this method. In this study, we report the influence that object morphology can have on qRT-PCR data. We have used a number of Arabidopsis thaliana mutants with altered floral morphology as models for this study. These mutants have been well characterised (including in terms of gene expression levels and patterns) by other techniques. This allows us to compare the results from the qRT-PCR with the results inferred from other methods. We demonstrate that the comparison of gene expression levels in objects that differ greatly in their morphology can lead to erroneous results.Natalia V DemidenkoAleksey A PeninPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e38161 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Natalia V Demidenko Aleksey A Penin Comparative analysis of gene expression level by quantitative real-time PCR has limited application in objects with different morphology.
description	qRT-PCR is a generally acknowledged method for gene expression analysis due to its precision and reproducibility. However, it is well known that the accuracy of qRT-PCR data varies greatly depending on the experimental design and data analysis. Recently, a set of guidelines has been proposed that aims to improve the reliability of qRT-PCR. However, there are additional factors that have not been taken into consideration in these guidelines that can seriously affect the data obtained using this method. In this study, we report the influence that object morphology can have on qRT-PCR data. We have used a number of Arabidopsis thaliana mutants with altered floral morphology as models for this study. These mutants have been well characterised (including in terms of gene expression levels and patterns) by other techniques. This allows us to compare the results from the qRT-PCR with the results inferred from other methods. We demonstrate that the comparison of gene expression levels in objects that differ greatly in their morphology can lead to erroneous results.
format	article
author	Natalia V Demidenko Aleksey A Penin
author_facet	Natalia V Demidenko Aleksey A Penin
author_sort	Natalia V Demidenko
title	Comparative analysis of gene expression level by quantitative real-time PCR has limited application in objects with different morphology.
title_short	Comparative analysis of gene expression level by quantitative real-time PCR has limited application in objects with different morphology.
title_full	Comparative analysis of gene expression level by quantitative real-time PCR has limited application in objects with different morphology.
title_fullStr	Comparative analysis of gene expression level by quantitative real-time PCR has limited application in objects with different morphology.
title_full_unstemmed	Comparative analysis of gene expression level by quantitative real-time PCR has limited application in objects with different morphology.
title_sort	comparative analysis of gene expression level by quantitative real-time pcr has limited application in objects with different morphology.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/2b087ac5091f40879687cb36006d1c25
work_keys_str_mv	AT nataliavdemidenko comparativeanalysisofgeneexpressionlevelbyquantitativerealtimepcrhaslimitedapplicationinobjectswithdifferentmorphology AT alekseyapenin comparativeanalysisofgeneexpressionlevelbyquantitativerealtimepcrhaslimitedapplicationinobjectswithdifferentmorphology
_version_	1718423652890836992

Comparative analysis of gene expression level by quantitative real-time PCR has limited application in objects with different morphology.

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