MiRNA-29c-3p Promotes Intestinal Inflammation via Targeting Leukemia Inhibitory Factor in Ulcerative Colitis

Jian Guo,1,2 Ruiya Zhang,3 Yiqing Zhao,3 Junping Wang3 1Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, 030006, People’s Republic of China; 2Department of General Surgery, Shanxi Provincial People’s Hospital, The...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Guo J, Zhang R, Zhao Y, Wang J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	ulcerative colitis microrna-29c-3p leukemia inhibitory factor inflammation apoptosis Pathology RB1-214 Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/2b11399139144b9cac05410289da4723
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2b11399139144b9cac05410289da4723
record_format	dspace
spelling	oai:doaj.org-article:2b11399139144b9cac05410289da47232021-12-02T15:55:56ZMiRNA-29c-3p Promotes Intestinal Inflammation via Targeting Leukemia Inhibitory Factor in Ulcerative Colitis1178-7031https://doaj.org/article/2b11399139144b9cac05410289da47232021-05-01T00:00:00Zhttps://www.dovepress.com/mirna-29c-3p-promotes-intestinal-inflammation-via-targeting-leukemia-i-peer-reviewed-fulltext-article-JIRhttps://doaj.org/toc/1178-7031Jian Guo,1,2 Ruiya Zhang,3 Yiqing Zhao,3 Junping Wang3 1Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, 030006, People’s Republic of China; 2Department of General Surgery, Shanxi Provincial People’s Hospital, The Affiliated People’s Hospital of Shanxi Medical University, Taiyuan, 030012, People’s Republic of China; 3Department of Gastroenterology, Shanxi Provincial People’s Hospital, The Affiliated People’s Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030012, People’s Republic of ChinaCorrespondence: Junping WangDepartment of Gastroenterology, Shanxi Provincial People’s Hospital, The Affiliated People’s Hospital of Shanxi Medical University, 29 Shuangtasi Road, Taiyuan, Shanxi, 030012, People’s Republic of ChinaTel + 351-4960130Email wermik527@21cn.comBackground: Dysregulation of micro-RNAs (miRNAs) is profoundly linked to inflammatory bowel diseases (IBD), but little is known about the specific biological functions of miRNAs in IBD. This study sought to elucidate the effect and the underlying target of miR-29c-3p in ulcerative colitis (UC).Methods: The levels of miR-29c-3p and leukemia inhibitory factor (LIF) were measured in inflamed lesions of UC patients and dextran sulfate sodium (DSS)-induced colitis mice by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. MiR-29c-3p was predicted to target LIF by bioinformatics software, which was verified via luciferase reporter assay and transfection of miR-29c-3p mimics or inhibitor. The role of miR-29c-3p/LIF axis in intestinal inflammation was explored in experimental colitis mice and Caco-2 cells.Results: MiR-29c-3p was markedly downregulated while LIF was upregulated in colon tissues of UC patients and DSS-challenged colitis mice as well as in primary intestinal epithelial cells (IECs) and LPS-treated Caco-2 cells. MiR-29c-3p inhibited LIF expression at the transcriptional level via binding to LIF 3ʹ-untranslated region (UTR) in Caco-2 cells. Targeting miR-29c-3p/LIF axis regulated inflammatory cytokines production, cell proliferation and apoptosis. Overexpression of miR-29c-3p aggravated mice experimental colitis via suppressing LIF.Conclusion: Our findings demonstrate that the upregulation of miR-29c-3p promotes gut inflammation and the expression of pro-inflammatory mediators via suppressing LIF, thereby modulating the pathogenesis of UC.Keywords: ulcerative colitis, microRNA-29c-3p, leukemia inhibitory factor, inflammation, apoptosisGuo JZhang RZhao YWang JDove Medical Pressarticleulcerative colitismicrorna-29c-3pleukemia inhibitory factorinflammationapoptosisPathologyRB1-214Therapeutics. PharmacologyRM1-950ENJournal of Inflammation Research, Vol Volume 14, Pp 2031-2043 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ulcerative colitis microrna-29c-3p leukemia inhibitory factor inflammation apoptosis Pathology RB1-214 Therapeutics. Pharmacology RM1-950
spellingShingle	ulcerative colitis microrna-29c-3p leukemia inhibitory factor inflammation apoptosis Pathology RB1-214 Therapeutics. Pharmacology RM1-950 Guo J Zhang R Zhao Y Wang J MiRNA-29c-3p Promotes Intestinal Inflammation via Targeting Leukemia Inhibitory Factor in Ulcerative Colitis
description	Jian Guo,1,2 Ruiya Zhang,3 Yiqing Zhao,3 Junping Wang3 1Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, 030006, People’s Republic of China; 2Department of General Surgery, Shanxi Provincial People’s Hospital, The Affiliated People’s Hospital of Shanxi Medical University, Taiyuan, 030012, People’s Republic of China; 3Department of Gastroenterology, Shanxi Provincial People’s Hospital, The Affiliated People’s Hospital of Shanxi Medical University, Taiyuan, Shanxi, 030012, People’s Republic of ChinaCorrespondence: Junping WangDepartment of Gastroenterology, Shanxi Provincial People’s Hospital, The Affiliated People’s Hospital of Shanxi Medical University, 29 Shuangtasi Road, Taiyuan, Shanxi, 030012, People’s Republic of ChinaTel + 351-4960130Email wermik527@21cn.comBackground: Dysregulation of micro-RNAs (miRNAs) is profoundly linked to inflammatory bowel diseases (IBD), but little is known about the specific biological functions of miRNAs in IBD. This study sought to elucidate the effect and the underlying target of miR-29c-3p in ulcerative colitis (UC).Methods: The levels of miR-29c-3p and leukemia inhibitory factor (LIF) were measured in inflamed lesions of UC patients and dextran sulfate sodium (DSS)-induced colitis mice by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. MiR-29c-3p was predicted to target LIF by bioinformatics software, which was verified via luciferase reporter assay and transfection of miR-29c-3p mimics or inhibitor. The role of miR-29c-3p/LIF axis in intestinal inflammation was explored in experimental colitis mice and Caco-2 cells.Results: MiR-29c-3p was markedly downregulated while LIF was upregulated in colon tissues of UC patients and DSS-challenged colitis mice as well as in primary intestinal epithelial cells (IECs) and LPS-treated Caco-2 cells. MiR-29c-3p inhibited LIF expression at the transcriptional level via binding to LIF 3ʹ-untranslated region (UTR) in Caco-2 cells. Targeting miR-29c-3p/LIF axis regulated inflammatory cytokines production, cell proliferation and apoptosis. Overexpression of miR-29c-3p aggravated mice experimental colitis via suppressing LIF.Conclusion: Our findings demonstrate that the upregulation of miR-29c-3p promotes gut inflammation and the expression of pro-inflammatory mediators via suppressing LIF, thereby modulating the pathogenesis of UC.Keywords: ulcerative colitis, microRNA-29c-3p, leukemia inhibitory factor, inflammation, apoptosis
format	article
author	Guo J Zhang R Zhao Y Wang J
author_facet	Guo J Zhang R Zhao Y Wang J
author_sort	Guo J
title	MiRNA-29c-3p Promotes Intestinal Inflammation via Targeting Leukemia Inhibitory Factor in Ulcerative Colitis
title_short	MiRNA-29c-3p Promotes Intestinal Inflammation via Targeting Leukemia Inhibitory Factor in Ulcerative Colitis
title_full	MiRNA-29c-3p Promotes Intestinal Inflammation via Targeting Leukemia Inhibitory Factor in Ulcerative Colitis
title_fullStr	MiRNA-29c-3p Promotes Intestinal Inflammation via Targeting Leukemia Inhibitory Factor in Ulcerative Colitis
title_full_unstemmed	MiRNA-29c-3p Promotes Intestinal Inflammation via Targeting Leukemia Inhibitory Factor in Ulcerative Colitis
title_sort	mirna-29c-3p promotes intestinal inflammation via targeting leukemia inhibitory factor in ulcerative colitis
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/2b11399139144b9cac05410289da4723
work_keys_str_mv	AT guoj mirna29c3ppromotesintestinalinflammationviatargetingleukemiainhibitoryfactorinulcerativecolitis AT zhangr mirna29c3ppromotesintestinalinflammationviatargetingleukemiainhibitoryfactorinulcerativecolitis AT zhaoy mirna29c3ppromotesintestinalinflammationviatargetingleukemiainhibitoryfactorinulcerativecolitis AT wangj mirna29c3ppromotesintestinalinflammationviatargetingleukemiainhibitoryfactorinulcerativecolitis
_version_	1718385381409292288

MiRNA-29c-3p Promotes Intestinal Inflammation via Targeting Leukemia Inhibitory Factor in Ulcerative Colitis

Ejemplares similares