Screening wild and cultivated cucurbits against root knot nematode to exploit as rootstocks for grafting in cucumber

Yield of mono-cropped cucumber (Cucumis sativus L.) is reduced by root knot nematode (Meloidogyne incognita Kofoid and White). Use of resistant rootstocks in grafting may overcome the problem. Cucurbitaceous species were screened against root knot nematode to evaluate their use as rootstocks in graf...

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Autores principales:	C Thangamani, L Pugalendhi, V Punithaveni
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2018
Materias:	meloidogyne incognita root knot nematode index phenols peroxidase ppo Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/2b167f3667f944f2ae61623647129b3b
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spelling	oai:doaj.org-article:2b167f3667f944f2ae61623647129b3b2021-12-02T12:07:32ZScreening wild and cultivated cucurbits against root knot nematode to exploit as rootstocks for grafting in cucumber0973-354X2582-4899https://doaj.org/article/2b167f3667f944f2ae61623647129b3b2018-06-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/28https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899Yield of mono-cropped cucumber (Cucumis sativus L.) is reduced by root knot nematode (Meloidogyne incognita Kofoid and White). Use of resistant rootstocks in grafting may overcome the problem. Cucurbitaceous species were screened against root knot nematode to evaluate their use as rootstocks in grafting. Inoculation was with nematodes @ 2 J2·g-1 (J2 = second stage juvenile) of soil in pot culture at the 1 to 2 true leaf stage, 45 days after inoculation, plants were uprooted and observations made to calculate Root Knot Nematode Index (RKI). Cucurbita moschata, Cucumis metuliferus, Citrullus colocynthis and Cucumis callosus were resistant having a RKI-2. Cucurbita ficifolia, Cucurbita maxima, Cucumis melo sub sp. agrestis were moderately resistant with a RKI-3. Total phenols content in roots indicates plant resistance to M. incognita. Cucumis metuliferus had the highest mean total phenols content (16.98 mg·g- 1 of root) followed by Citrullus colocynthis (16.08 mg·g-1 of root) and Cucurbita moschata (15.37mg·g-1 of root). Resistant rootstocks possessed higher peroxidase and PPO activity than susceptible ones. Cucumis metuliferus had the highest value of peroxidase and PPO activity (3.83 OD·min-1·g-1 of root and 3.67 OD·min-1·g-1 of root) followed by Citrullus colocynthis (3.26 and 3.63 OD·min-1·g-1 of root), Cucumis callosus (3.02 and 2.98 OD·min-1·g-1 of root) and Cucurbita moschata (2.93 and 2.94 OD·min-1·g-1 of root). Cucumber scions, ‘Green Long’ and ‘NS 408’ had lower peroxidase and PPO activity of 0.64 and 1.42 OD·min-1·g-1 and 0.57 and 1.31 OD·min- 1·g-1 of root, respectively. Resistant and moderately resistant cucurbitaceous species may be used for further studies possibly leading to improved yield.C ThangamaniL PugalendhiV PunithaveniSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticlemeloidogyne incognitaroot knot nematode indexphenolsperoxidaseppoPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 13, Iss 1, Pp 32-41 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	meloidogyne incognita root knot nematode index phenols peroxidase ppo Plant culture SB1-1110
spellingShingle	meloidogyne incognita root knot nematode index phenols peroxidase ppo Plant culture SB1-1110 C Thangamani L Pugalendhi V Punithaveni Screening wild and cultivated cucurbits against root knot nematode to exploit as rootstocks for grafting in cucumber
description	Yield of mono-cropped cucumber (Cucumis sativus L.) is reduced by root knot nematode (Meloidogyne incognita Kofoid and White). Use of resistant rootstocks in grafting may overcome the problem. Cucurbitaceous species were screened against root knot nematode to evaluate their use as rootstocks in grafting. Inoculation was with nematodes @ 2 J2·g-1 (J2 = second stage juvenile) of soil in pot culture at the 1 to 2 true leaf stage, 45 days after inoculation, plants were uprooted and observations made to calculate Root Knot Nematode Index (RKI). Cucurbita moschata, Cucumis metuliferus, Citrullus colocynthis and Cucumis callosus were resistant having a RKI-2. Cucurbita ficifolia, Cucurbita maxima, Cucumis melo sub sp. agrestis were moderately resistant with a RKI-3. Total phenols content in roots indicates plant resistance to M. incognita. Cucumis metuliferus had the highest mean total phenols content (16.98 mg·g- 1 of root) followed by Citrullus colocynthis (16.08 mg·g-1 of root) and Cucurbita moschata (15.37mg·g-1 of root). Resistant rootstocks possessed higher peroxidase and PPO activity than susceptible ones. Cucumis metuliferus had the highest value of peroxidase and PPO activity (3.83 OD·min-1·g-1 of root and 3.67 OD·min-1·g-1 of root) followed by Citrullus colocynthis (3.26 and 3.63 OD·min-1·g-1 of root), Cucumis callosus (3.02 and 2.98 OD·min-1·g-1 of root) and Cucurbita moschata (2.93 and 2.94 OD·min-1·g-1 of root). Cucumber scions, ‘Green Long’ and ‘NS 408’ had lower peroxidase and PPO activity of 0.64 and 1.42 OD·min-1·g-1 and 0.57 and 1.31 OD·min- 1·g-1 of root, respectively. Resistant and moderately resistant cucurbitaceous species may be used for further studies possibly leading to improved yield.
format	article
author	C Thangamani L Pugalendhi V Punithaveni
author_facet	C Thangamani L Pugalendhi V Punithaveni
author_sort	C Thangamani
title	Screening wild and cultivated cucurbits against root knot nematode to exploit as rootstocks for grafting in cucumber
title_short	Screening wild and cultivated cucurbits against root knot nematode to exploit as rootstocks for grafting in cucumber
title_full	Screening wild and cultivated cucurbits against root knot nematode to exploit as rootstocks for grafting in cucumber
title_fullStr	Screening wild and cultivated cucurbits against root knot nematode to exploit as rootstocks for grafting in cucumber
title_full_unstemmed	Screening wild and cultivated cucurbits against root knot nematode to exploit as rootstocks for grafting in cucumber
title_sort	screening wild and cultivated cucurbits against root knot nematode to exploit as rootstocks for grafting in cucumber
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2018
url	https://doaj.org/article/2b167f3667f944f2ae61623647129b3b
work_keys_str_mv	AT cthangamani screeningwildandcultivatedcucurbitsagainstrootknotnematodetoexploitasrootstocksforgraftingincucumber AT lpugalendhi screeningwildandcultivatedcucurbitsagainstrootknotnematodetoexploitasrootstocksforgraftingincucumber AT vpunithaveni screeningwildandcultivatedcucurbitsagainstrootknotnematodetoexploitasrootstocksforgraftingincucumber
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Screening wild and cultivated cucurbits against root knot nematode to exploit as rootstocks for grafting in cucumber

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