Stigmasterol Exerts Neuro-Protective Effect Against Ischemic/Reperfusion Injury Through Reduction Of Oxidative Stress And Inactivation Of Autophagy

Jiadong Sun, Xuemei Li, Junling Liu, Xin Pan, Qianqian Zhao Department of Neurology, Affiliated Hospital of Weifang Medical University, Weifang City, Shandong Province 262100, People’s Republic of ChinaCorrespondence: Jiadong SunDepartment of Neurology, Affiliated Hospital of Weifang Medic...

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Autores principales:	Sun J, Li X, Liu J, Pan X, Zhao Q
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Stigmasterol ischemia/reperfusion injury oxidative stress autophagy AMPK/mTOR and JNK pathways Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/2b1a2933b48843c1bb9dc04a3ef36167
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spelling	oai:doaj.org-article:2b1a2933b48843c1bb9dc04a3ef361672021-12-02T05:20:16ZStigmasterol Exerts Neuro-Protective Effect Against Ischemic/Reperfusion Injury Through Reduction Of Oxidative Stress And Inactivation Of Autophagy1178-2021https://doaj.org/article/2b1a2933b48843c1bb9dc04a3ef361672019-10-01T00:00:00Zhttps://www.dovepress.com/stigmasterol-exerts-neuro-protective-effect-against-ischemicreperfusio-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Jiadong Sun, Xuemei Li, Junling Liu, Xin Pan, Qianqian Zhao Department of Neurology, Affiliated Hospital of Weifang Medical University, Weifang City, Shandong Province 262100, People’s Republic of ChinaCorrespondence: Jiadong SunDepartment of Neurology, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Weifang City, Shandong Province 262100, People’s Republic of ChinaTel +86 536 3081268Email wsmztrusnx5@163.comPurpose: Stroke remains the primary cause of pain, suffering, and death in patients. One of the major thrusts in stroke therapy is to find an effective prevention strategy. Objectives of this study are to testify the neuro-protection effect of stigmasterol in ischemic/reperfusion injury model.Methods: The dosage-dependent effects (20, 40, and 80 mg/kg) of stigmasterol on physiological behaviors and oxidative stress biomarkers were investigated. Expression and phosphorylation of beclin1, microtubule-associated protein 1 light chain 3 (LC3), adenosine monophosphate-activated protein kinase (AMPK), mTOR, and N-terminal kinase (JNK) were detected.Results: The results showed that stigmasterol was able to effectively reduce neurological deficits and infarct damage induced by the ischemic/reperfusion injury, improve histopathology changes, and restore the levels of the endogenous antioxidant defense system in a dose–response mode. Stigmasterol effectively depressed the expression level of beclin1, and the conversion of LC3 I to LC3 II, while promoted the phosphorylation of mTOR, and remarkably inhibited the phosphorylation of AMPK and JNK, as well as the expression of JNK induced by 24 hrs of reperfusion.Conclusion: These findings reveal that stigmasterol has neuro-protective effect against the ischemic/reperfusion injury, possibly associated with reduction of oxidative stress and inactivation of autophagy via AMPK/mTOR and JNK pathways.Keywords: stigmasterol, ischemia/reperfusion injury, oxidative stress, autophagy, AMPK pathway, mTOR pathway, JNK pathway  Sun JLi XLiu JPan XZhao QDove Medical PressarticleStigmasterolischemia/reperfusion injuryoxidative stressautophagyAMPK/mTOR and JNK pathwaysNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 15, Pp 2991-3001 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Stigmasterol ischemia/reperfusion injury oxidative stress autophagy AMPK/mTOR and JNK pathways Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429
spellingShingle	Stigmasterol ischemia/reperfusion injury oxidative stress autophagy AMPK/mTOR and JNK pathways Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Neurology. Diseases of the nervous system RC346-429 Sun J Li X Liu J Pan X Zhao Q Stigmasterol Exerts Neuro-Protective Effect Against Ischemic/Reperfusion Injury Through Reduction Of Oxidative Stress And Inactivation Of Autophagy
description	Jiadong Sun, Xuemei Li, Junling Liu, Xin Pan, Qianqian Zhao Department of Neurology, Affiliated Hospital of Weifang Medical University, Weifang City, Shandong Province 262100, People’s Republic of ChinaCorrespondence: Jiadong SunDepartment of Neurology, Affiliated Hospital of Weifang Medical University, No. 2428 Yuhe Road, Weifang City, Shandong Province 262100, People’s Republic of ChinaTel +86 536 3081268Email wsmztrusnx5@163.comPurpose: Stroke remains the primary cause of pain, suffering, and death in patients. One of the major thrusts in stroke therapy is to find an effective prevention strategy. Objectives of this study are to testify the neuro-protection effect of stigmasterol in ischemic/reperfusion injury model.Methods: The dosage-dependent effects (20, 40, and 80 mg/kg) of stigmasterol on physiological behaviors and oxidative stress biomarkers were investigated. Expression and phosphorylation of beclin1, microtubule-associated protein 1 light chain 3 (LC3), adenosine monophosphate-activated protein kinase (AMPK), mTOR, and N-terminal kinase (JNK) were detected.Results: The results showed that stigmasterol was able to effectively reduce neurological deficits and infarct damage induced by the ischemic/reperfusion injury, improve histopathology changes, and restore the levels of the endogenous antioxidant defense system in a dose–response mode. Stigmasterol effectively depressed the expression level of beclin1, and the conversion of LC3 I to LC3 II, while promoted the phosphorylation of mTOR, and remarkably inhibited the phosphorylation of AMPK and JNK, as well as the expression of JNK induced by 24 hrs of reperfusion.Conclusion: These findings reveal that stigmasterol has neuro-protective effect against the ischemic/reperfusion injury, possibly associated with reduction of oxidative stress and inactivation of autophagy via AMPK/mTOR and JNK pathways.Keywords: stigmasterol, ischemia/reperfusion injury, oxidative stress, autophagy, AMPK pathway, mTOR pathway, JNK pathway
format	article
author	Sun J Li X Liu J Pan X Zhao Q
author_facet	Sun J Li X Liu J Pan X Zhao Q
author_sort	Sun J
title	Stigmasterol Exerts Neuro-Protective Effect Against Ischemic/Reperfusion Injury Through Reduction Of Oxidative Stress And Inactivation Of Autophagy
title_short	Stigmasterol Exerts Neuro-Protective Effect Against Ischemic/Reperfusion Injury Through Reduction Of Oxidative Stress And Inactivation Of Autophagy
title_full	Stigmasterol Exerts Neuro-Protective Effect Against Ischemic/Reperfusion Injury Through Reduction Of Oxidative Stress And Inactivation Of Autophagy
title_fullStr	Stigmasterol Exerts Neuro-Protective Effect Against Ischemic/Reperfusion Injury Through Reduction Of Oxidative Stress And Inactivation Of Autophagy
title_full_unstemmed	Stigmasterol Exerts Neuro-Protective Effect Against Ischemic/Reperfusion Injury Through Reduction Of Oxidative Stress And Inactivation Of Autophagy
title_sort	stigmasterol exerts neuro-protective effect against ischemic/reperfusion injury through reduction of oxidative stress and inactivation of autophagy
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/2b1a2933b48843c1bb9dc04a3ef36167
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_version_	1718400459900715008

Stigmasterol Exerts Neuro-Protective Effect Against Ischemic/Reperfusion Injury Through Reduction Of Oxidative Stress And Inactivation Of Autophagy

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