Echogenic, Ultrasound-Sensitive Chitosan Nanodroplets for Spatiotemporally Controlled DKK-2 Gene Delivery to Prostate Cancer Cells

Xinxin Liu, Dandan Shi, Lu Guo, Xiaoying Zhou, Mengmeng Shang, Xiao Sun, Dong Meng, Yading Zhao, Jie Li Department of Ultrasound, Qilu Hospital of Shandong University, Jinan, People’s Republic of ChinaCorrespondence: Jie LiDepartment of Ultrasound, Qilu Hospital of Shandong University, Wen...

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Autores principales:	Liu X, Shi D, Guo L, Zhou X, Shang M, Sun X, Meng D, Zhao Y, Li J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	nanodroplets chitosan dkk-2 ultrasonic gene transfer prostate cancer Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2b1ae9af6f6741d8832c01f02431db30
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spelling	oai:doaj.org-article:2b1ae9af6f6741d8832c01f02431db302021-12-02T15:59:41ZEchogenic, Ultrasound-Sensitive Chitosan Nanodroplets for Spatiotemporally Controlled DKK-2 Gene Delivery to Prostate Cancer Cells1178-2013https://doaj.org/article/2b1ae9af6f6741d8832c01f02431db302021-01-01T00:00:00Zhttps://www.dovepress.com/echogenic-ultrasound-sensitive-chitosan-nanodroplets-for-spatiotempora-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xinxin Liu, Dandan Shi, Lu Guo, Xiaoying Zhou, Mengmeng Shang, Xiao Sun, Dong Meng, Yading Zhao, Jie Li Department of Ultrasound, Qilu Hospital of Shandong University, Jinan, People’s Republic of ChinaCorrespondence: Jie LiDepartment of Ultrasound, Qilu Hospital of Shandong University, Wenhua Western Road, Jinan 107#, People’s Republic of ChinaTel/Fax +86-531-82166101Email jieli301@163.comPurpose: To synthesize echogenic chitosan/perﬂuorohexane nanodroplets (CNDs) for DKK-2 gene delivering in a spatiotemporally controlled manner in vitro.Methods: The characteristics, contrast-enhanced ultrasound imaging, DNA binding and DNase protection capacity, DKK-2 gene transfection and effects on LNCaP cells of these CNDs were investigated.Results: The obtained CNDs showed positive surface charges and could attract the genetic cargo with negative surface charges to form nanocomplexes. Agarose gel electrophoresis confirmed binding of the CNDs and pDNA. DKK-2 pDNA-loaded CNDs, in combination with ultrasound, ruptured and released DKK-2 pDNA, entering LNCaP cells through nano-scale pores in the cell membrane, which further reduced the proliferation of LNCaP cells.Conclusion: These stable and safe CNDs may be a promising choice to achieve efficient ultrasound-mediated gene delivery to specific tissues in a spatiotemporally controlled manner.Keywords: nanodroplets, chitosan, DKK-2, ultrasonic gene transfer, prostate cancerLiu XShi DGuo LZhou XShang MSun XMeng DZhao YLi JDove Medical Pressarticlenanodropletschitosandkk-2ultrasonic gene transferprostate cancerMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 421-432 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanodroplets chitosan dkk-2 ultrasonic gene transfer prostate cancer Medicine (General) R5-920
spellingShingle	nanodroplets chitosan dkk-2 ultrasonic gene transfer prostate cancer Medicine (General) R5-920 Liu X Shi D Guo L Zhou X Shang M Sun X Meng D Zhao Y Li J Echogenic, Ultrasound-Sensitive Chitosan Nanodroplets for Spatiotemporally Controlled DKK-2 Gene Delivery to Prostate Cancer Cells
description	Xinxin Liu, Dandan Shi, Lu Guo, Xiaoying Zhou, Mengmeng Shang, Xiao Sun, Dong Meng, Yading Zhao, Jie Li Department of Ultrasound, Qilu Hospital of Shandong University, Jinan, People’s Republic of ChinaCorrespondence: Jie LiDepartment of Ultrasound, Qilu Hospital of Shandong University, Wenhua Western Road, Jinan 107#, People’s Republic of ChinaTel/Fax +86-531-82166101Email jieli301@163.comPurpose: To synthesize echogenic chitosan/perﬂuorohexane nanodroplets (CNDs) for DKK-2 gene delivering in a spatiotemporally controlled manner in vitro.Methods: The characteristics, contrast-enhanced ultrasound imaging, DNA binding and DNase protection capacity, DKK-2 gene transfection and effects on LNCaP cells of these CNDs were investigated.Results: The obtained CNDs showed positive surface charges and could attract the genetic cargo with negative surface charges to form nanocomplexes. Agarose gel electrophoresis confirmed binding of the CNDs and pDNA. DKK-2 pDNA-loaded CNDs, in combination with ultrasound, ruptured and released DKK-2 pDNA, entering LNCaP cells through nano-scale pores in the cell membrane, which further reduced the proliferation of LNCaP cells.Conclusion: These stable and safe CNDs may be a promising choice to achieve efficient ultrasound-mediated gene delivery to specific tissues in a spatiotemporally controlled manner.Keywords: nanodroplets, chitosan, DKK-2, ultrasonic gene transfer, prostate cancer
format	article
author	Liu X Shi D Guo L Zhou X Shang M Sun X Meng D Zhao Y Li J
author_facet	Liu X Shi D Guo L Zhou X Shang M Sun X Meng D Zhao Y Li J
author_sort	Liu X
title	Echogenic, Ultrasound-Sensitive Chitosan Nanodroplets for Spatiotemporally Controlled DKK-2 Gene Delivery to Prostate Cancer Cells
title_short	Echogenic, Ultrasound-Sensitive Chitosan Nanodroplets for Spatiotemporally Controlled DKK-2 Gene Delivery to Prostate Cancer Cells
title_full	Echogenic, Ultrasound-Sensitive Chitosan Nanodroplets for Spatiotemporally Controlled DKK-2 Gene Delivery to Prostate Cancer Cells
title_fullStr	Echogenic, Ultrasound-Sensitive Chitosan Nanodroplets for Spatiotemporally Controlled DKK-2 Gene Delivery to Prostate Cancer Cells
title_full_unstemmed	Echogenic, Ultrasound-Sensitive Chitosan Nanodroplets for Spatiotemporally Controlled DKK-2 Gene Delivery to Prostate Cancer Cells
title_sort	echogenic, ultrasound-sensitive chitosan nanodroplets for spatiotemporally controlled dkk-2 gene delivery to prostate cancer cells
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/2b1ae9af6f6741d8832c01f02431db30
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Echogenic, Ultrasound-Sensitive Chitosan Nanodroplets for Spatiotemporally Controlled DKK-2 Gene Delivery to Prostate Cancer Cells

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