Skeletal muscle metabolic responses to physical activity are muscle type specific in a rat model of chronic kidney disease

Abstract Chronic kidney disease (CKD) leads to musculoskeletal impairments that are impacted by muscle metabolism. We tested the hypothesis that 10-weeks of voluntary wheel running can improve skeletal muscle mitochondria activity and function in a rat model of CKD. Groups included (n = 12–14/group)...

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Autores principales:	Keith G. Avin, Meghan C. Hughes, Neal X. Chen, Shruthi Srinivasan, Kalisha D. O’Neill, Andrew P. Evan, Robert L. Bacallao, Michael L. Schulte, Ranjani N. Moorthi, Debora L. Gisch, Christopher G. R. Perry, Sharon M. Moe, Thomas M. O’Connell
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b201539047144c29d1abd2252d93f36
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spelling	oai:doaj.org-article:2b201539047144c29d1abd2252d93f362021-12-02T14:49:25ZSkeletal muscle metabolic responses to physical activity are muscle type specific in a rat model of chronic kidney disease10.1038/s41598-021-89120-82045-2322https://doaj.org/article/2b201539047144c29d1abd2252d93f362021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89120-8https://doaj.org/toc/2045-2322Abstract Chronic kidney disease (CKD) leads to musculoskeletal impairments that are impacted by muscle metabolism. We tested the hypothesis that 10-weeks of voluntary wheel running can improve skeletal muscle mitochondria activity and function in a rat model of CKD. Groups included (n = 12–14/group): (1) normal littermates (NL); (2) CKD, and; (3) CKD-10 weeks of voluntary wheel running (CKD-W). At 35-weeks old the following assays were performed in the soleus and extensor digitorum longus (EDL): targeted metabolomics, mitochondrial respiration, and protein expression. Amino acid-related compounds were reduced in CKD muscle and not restored by physical activity. Mitochondrial respiration in the CKD soleus was increased compared to NL, but not impacted by physical activity. The EDL respiration was not different between NL and CKD, but increased in CKD-wheel rats compared to CKD and NL groups. Our results demonstrate that the soleus may be more susceptible to CKD-induced changes of mitochondrial complex content and respiration, while in the EDL, these alterations were in response the physiological load induced by mild physical activity. Future studies should focus on therapies to improve mitochondrial function in both types of muscle to determine if such treatments can improve the ability to adapt to physical activity in CKD.Keith G. AvinMeghan C. HughesNeal X. ChenShruthi SrinivasanKalisha D. O’NeillAndrew P. EvanRobert L. BacallaoMichael L. SchulteRanjani N. MoorthiDebora L. GischChristopher G. R. PerrySharon M. MoeThomas M. O’ConnellNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keith G. Avin Meghan C. Hughes Neal X. Chen Shruthi Srinivasan Kalisha D. O’Neill Andrew P. Evan Robert L. Bacallao Michael L. Schulte Ranjani N. Moorthi Debora L. Gisch Christopher G. R. Perry Sharon M. Moe Thomas M. O’Connell Skeletal muscle metabolic responses to physical activity are muscle type specific in a rat model of chronic kidney disease
description	Abstract Chronic kidney disease (CKD) leads to musculoskeletal impairments that are impacted by muscle metabolism. We tested the hypothesis that 10-weeks of voluntary wheel running can improve skeletal muscle mitochondria activity and function in a rat model of CKD. Groups included (n = 12–14/group): (1) normal littermates (NL); (2) CKD, and; (3) CKD-10 weeks of voluntary wheel running (CKD-W). At 35-weeks old the following assays were performed in the soleus and extensor digitorum longus (EDL): targeted metabolomics, mitochondrial respiration, and protein expression. Amino acid-related compounds were reduced in CKD muscle and not restored by physical activity. Mitochondrial respiration in the CKD soleus was increased compared to NL, but not impacted by physical activity. The EDL respiration was not different between NL and CKD, but increased in CKD-wheel rats compared to CKD and NL groups. Our results demonstrate that the soleus may be more susceptible to CKD-induced changes of mitochondrial complex content and respiration, while in the EDL, these alterations were in response the physiological load induced by mild physical activity. Future studies should focus on therapies to improve mitochondrial function in both types of muscle to determine if such treatments can improve the ability to adapt to physical activity in CKD.
format	article
author	Keith G. Avin Meghan C. Hughes Neal X. Chen Shruthi Srinivasan Kalisha D. O’Neill Andrew P. Evan Robert L. Bacallao Michael L. Schulte Ranjani N. Moorthi Debora L. Gisch Christopher G. R. Perry Sharon M. Moe Thomas M. O’Connell
author_facet	Keith G. Avin Meghan C. Hughes Neal X. Chen Shruthi Srinivasan Kalisha D. O’Neill Andrew P. Evan Robert L. Bacallao Michael L. Schulte Ranjani N. Moorthi Debora L. Gisch Christopher G. R. Perry Sharon M. Moe Thomas M. O’Connell
author_sort	Keith G. Avin
title	Skeletal muscle metabolic responses to physical activity are muscle type specific in a rat model of chronic kidney disease
title_short	Skeletal muscle metabolic responses to physical activity are muscle type specific in a rat model of chronic kidney disease
title_full	Skeletal muscle metabolic responses to physical activity are muscle type specific in a rat model of chronic kidney disease
title_fullStr	Skeletal muscle metabolic responses to physical activity are muscle type specific in a rat model of chronic kidney disease
title_full_unstemmed	Skeletal muscle metabolic responses to physical activity are muscle type specific in a rat model of chronic kidney disease
title_sort	skeletal muscle metabolic responses to physical activity are muscle type specific in a rat model of chronic kidney disease
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2b201539047144c29d1abd2252d93f36
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Skeletal muscle metabolic responses to physical activity are muscle type specific in a rat model of chronic kidney disease

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