Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer

Abstract In worldwide conditions of increasingly antibiotic-resistant hospital infections, it is important to research alternative therapies. Bdellovibrio bacteriovorus bacteria naturally prey on Gram-negative pathogens, including antibiotic-resistant strains and so B. bacteriovorus have been propos...

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Autores principales:	Michelle Baker, David Negus, Dhaarini Raghunathan, Paul Radford, Chris Moore, Gemma Clark, Mathew Diggle, Jess Tyson, Jamie Twycross, R. Elizabeth Sockett
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2b270bd58fcc40439564a681909ad145
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spelling	oai:doaj.org-article:2b270bd58fcc40439564a681909ad1452021-12-02T16:07:45ZMeasuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer10.1038/s41598-017-08060-42045-2322https://doaj.org/article/2b270bd58fcc40439564a681909ad1452017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08060-4https://doaj.org/toc/2045-2322Abstract In worldwide conditions of increasingly antibiotic-resistant hospital infections, it is important to research alternative therapies. Bdellovibrio bacteriovorus bacteria naturally prey on Gram-negative pathogens, including antibiotic-resistant strains and so B. bacteriovorus have been proposed as “living antibiotics” to combat antimicrobially-resistant pathogens. Predator-prey interactions are complex and can be altered by environmental components. To be effective B. bacteriovorus predation needs to work in human body fluids such as serum where predation dynamics may differ to that studied in laboratory media. Here we combine mathematical modelling and lab experimentation to investigate the predation of an important carbapenem-resistant human pathogen, Klebsiella pneumoniae, by B. bacteriovorus in human serum versus buffer. We show experimentally that B. bacteriovorus is able to reduce prey numbers in each environment, on different timescales. Our mathematical model captures the underlying dynamics of the experimentation, including an initial predation-delay at the predator-prey-serum interface. Our research shows differences between predation in buffer and serum and highlights both the potential and limitations of B. bacteriovorus acting therapeutically against K. pneumoniae in serum, informing future research into the medicinal behaviours and dosing of this living antibacterial.Michelle BakerDavid NegusDhaarini RaghunathanPaul RadfordChris MooreGemma ClarkMathew DiggleJess TysonJamie TwycrossR. Elizabeth SockettNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-18 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Michelle Baker David Negus Dhaarini Raghunathan Paul Radford Chris Moore Gemma Clark Mathew Diggle Jess Tyson Jamie Twycross R. Elizabeth Sockett Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer
description	Abstract In worldwide conditions of increasingly antibiotic-resistant hospital infections, it is important to research alternative therapies. Bdellovibrio bacteriovorus bacteria naturally prey on Gram-negative pathogens, including antibiotic-resistant strains and so B. bacteriovorus have been proposed as “living antibiotics” to combat antimicrobially-resistant pathogens. Predator-prey interactions are complex and can be altered by environmental components. To be effective B. bacteriovorus predation needs to work in human body fluids such as serum where predation dynamics may differ to that studied in laboratory media. Here we combine mathematical modelling and lab experimentation to investigate the predation of an important carbapenem-resistant human pathogen, Klebsiella pneumoniae, by B. bacteriovorus in human serum versus buffer. We show experimentally that B. bacteriovorus is able to reduce prey numbers in each environment, on different timescales. Our mathematical model captures the underlying dynamics of the experimentation, including an initial predation-delay at the predator-prey-serum interface. Our research shows differences between predation in buffer and serum and highlights both the potential and limitations of B. bacteriovorus acting therapeutically against K. pneumoniae in serum, informing future research into the medicinal behaviours and dosing of this living antibacterial.
format	article
author	Michelle Baker David Negus Dhaarini Raghunathan Paul Radford Chris Moore Gemma Clark Mathew Diggle Jess Tyson Jamie Twycross R. Elizabeth Sockett
author_facet	Michelle Baker David Negus Dhaarini Raghunathan Paul Radford Chris Moore Gemma Clark Mathew Diggle Jess Tyson Jamie Twycross R. Elizabeth Sockett
author_sort	Michelle Baker
title	Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer
title_short	Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer
title_full	Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer
title_fullStr	Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer
title_full_unstemmed	Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer
title_sort	measuring and modelling the response of klebsiella pneumoniae kpc prey to bdellovibrio bacteriovorus predation, in human serum and defined buffer
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2b270bd58fcc40439564a681909ad145
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Measuring and modelling the response of Klebsiella pneumoniae KPC prey to Bdellovibrio bacteriovorus predation, in human serum and defined buffer

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